Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation
The mechanisms that underlie the potent Th1-adjuvant capacity of poly(methyl vinyl ether-co-maleic anhydride) nanoparticles (NPs) were investigated. Traditionally, polymer NPs have been considered delivery systems that promote a closer interaction between antigen and antigen-presenting cells (APCs...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society of Microbiology
2011
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| Online Access: | https://hdl.handle.net/10171/17103 |
| _version_ | 1793400144524214272 |
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| author | Tamayo, I. (Ibai) Irache, J.M. (Juan Manuel) Mansilla, C. (Cristina) Ochoa-Repáraz, J. (Javier) Lasarte, J.J. (Juan José) Gamazo, C. (Carlos) |
| author_facet | Tamayo, I. (Ibai) Irache, J.M. (Juan Manuel) Mansilla, C. (Cristina) Ochoa-Repáraz, J. (Javier) Lasarte, J.J. (Juan José) Gamazo, C. (Carlos) |
| author_sort | Tamayo, I. (Ibai) |
| collection | DSpace |
| description | The mechanisms that underlie the potent Th1-adjuvant capacity of poly(methyl vinyl ether-co-maleic anhydride)
nanoparticles (NPs) were investigated. Traditionally, polymer NPs have been considered delivery
systems that promote a closer interaction between antigen and antigen-presenting cells (APCs). Our results
revealed that poly(anhydride) NPs also act as agonists of various Toll-like receptors (TLRs) (TLR2, -4, and -5),
triggering a Th1-profile cytokine release (gamma interferon [IFN- ], 478 pg/ml versus 39.6 pg/ml from
negative control; interleukin-12 [IL-12], 40 pg/ml versus 7.2 pg/ml from negative control) and, after incubation
with dendritic cells, inducing a 2.5- to 3.5-fold increase of CD54 and CD86 costimulatory molecule expression.
Furthermore, in vivo studies suggest that NPs actively elicit a CD8 T-cell response. Immunization with empty
NPs resulted in a significant delay in the mean survival date (from day 7 until day 23 postchallenge) and a
protection level of 30% after challenge against a lethal dose of Salmonella enterica serovar Enteritidis. Taken
together, our results provide a better understanding of how NPs act as active Th1 adjuvants in immunoprophylaxis
and immunotherapy through TLR exploitation. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-17103 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2011 |
| publisher | American Society of Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-171032020-03-03T19:54:25Z Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation Tamayo, I. (Ibai) Irache, J.M. (Juan Manuel) Mansilla, C. (Cristina) Ochoa-Repáraz, J. (Javier) Lasarte, J.J. (Juan José) Gamazo, C. (Carlos) Nanoparticles Polyanhydride Immunology The mechanisms that underlie the potent Th1-adjuvant capacity of poly(methyl vinyl ether-co-maleic anhydride) nanoparticles (NPs) were investigated. Traditionally, polymer NPs have been considered delivery systems that promote a closer interaction between antigen and antigen-presenting cells (APCs). Our results revealed that poly(anhydride) NPs also act as agonists of various Toll-like receptors (TLRs) (TLR2, -4, and -5), triggering a Th1-profile cytokine release (gamma interferon [IFN- ], 478 pg/ml versus 39.6 pg/ml from negative control; interleukin-12 [IL-12], 40 pg/ml versus 7.2 pg/ml from negative control) and, after incubation with dendritic cells, inducing a 2.5- to 3.5-fold increase of CD54 and CD86 costimulatory molecule expression. Furthermore, in vivo studies suggest that NPs actively elicit a CD8 T-cell response. Immunization with empty NPs resulted in a significant delay in the mean survival date (from day 7 until day 23 postchallenge) and a protection level of 30% after challenge against a lethal dose of Salmonella enterica serovar Enteritidis. Taken together, our results provide a better understanding of how NPs act as active Th1 adjuvants in immunoprophylaxis and immunotherapy through TLR exploitation. 2011-03-18T13:14:09Z 2011-03-18T13:14:09Z 2010 info:eu-repo/semantics/article https://hdl.handle.net/10171/17103 eng info:eu-repo/semantics/openAccess application/pdf American Society of Microbiology |
| spellingShingle | Nanoparticles Polyanhydride Immunology Tamayo, I. (Ibai) Irache, J.M. (Juan Manuel) Mansilla, C. (Cristina) Ochoa-Repáraz, J. (Javier) Lasarte, J.J. (Juan José) Gamazo, C. (Carlos) Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation |
| title | Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation |
| title_full | Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation |
| title_fullStr | Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation |
| title_full_unstemmed | Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation |
| title_short | Poly(Anhydride) Nanoparticles Act as Active Th1 Adjuvants through Toll-Like Receptor Exploitation |
| title_sort | poly(anhydride) nanoparticles act as active th1 adjuvants through toll-like receptor exploitation |
| topic | Nanoparticles Polyanhydride Immunology |
| url | https://hdl.handle.net/10171/17103 |
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