Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato
Bisacylimidoselenocarbamate derivatives (BSC) are potent anticancer agents with a strong cytotoxic activity against different types of tumour cells. Based in phosphatidylserine exposure on the cell membranes we show that BSC treatment resulted in enhanced cell death in leukaemia CCRF-CEM cells. DNA...
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| Format: | info:eu-repo/semantics/doctoralThesis |
| Language: | spa |
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Universidad de Navarra
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/28202 |
| _version_ | 1793400498212044800 |
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| author | Lamberto, I. (Iranzu) Palop-Cubillo, J.A. (Juan Antonio) Encío, I. (Ignacio) |
| author_facet | Lamberto, I. (Iranzu) Palop-Cubillo, J.A. (Juan Antonio) Encío, I. (Ignacio) |
| author_sort | Lamberto, I. (Iranzu) |
| collection | DSpace |
| description | Bisacylimidoselenocarbamate derivatives (BSC) are potent anticancer agents with a strong cytotoxic activity against different types of tumour cells. Based in phosphatidylserine exposure on the cell membranes we show that BSC treatment resulted in enhanced cell death in leukaemia CCRF-CEM cells. DNA fragmentation detection in breast adenocarcinoma MCF-7 cells showed that BSC triggered cell death is concentration and time dependent. We also show that two of these compounds, BSC 3g and 3n, cause cell-cycle arrest in the late G2/M in MCF-7 cells. Consistent with this, a reduction in CDK1 and CDK2 expression with no change in cyclin A and B1 was observed in this cell line. Activation of caspase-2 was also detected. However, the involvement of the caspase-dependent pathway in the process of cell death induced by either BSC 3g or 3n is discarded since cell death could not be prevented by pretreatment with the pancaspase inhibitor z-VAD-fmk. Moreover, since reduced levels of p21CIP and Chk2 proteins but no change in p53 levels could be detected in MCF-7 cells after BSC 3g or 3n treatment our results suggest that BSC treated cells die from lethal mitosis. |
| format | info:eu-repo/semantics/doctoralThesis |
| id | oai:dadun.unav.edu:10171-28202 |
| institution | Universidad de Navarra |
| language | spa |
| publishDate | 2013 |
| publisher | Universidad de Navarra |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-282022021-10-20T10:41:43Z Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato Lamberto, I. (Iranzu) Palop-Cubillo, J.A. (Juan Antonio) Encío, I. (Ignacio) Materias Investigacion::Ciencias de la vida::Bioquímica Cultivo celular Biología molecular Química orgánica Bisacylimidoselenocarbamate derivatives (BSC) are potent anticancer agents with a strong cytotoxic activity against different types of tumour cells. Based in phosphatidylserine exposure on the cell membranes we show that BSC treatment resulted in enhanced cell death in leukaemia CCRF-CEM cells. DNA fragmentation detection in breast adenocarcinoma MCF-7 cells showed that BSC triggered cell death is concentration and time dependent. We also show that two of these compounds, BSC 3g and 3n, cause cell-cycle arrest in the late G2/M in MCF-7 cells. Consistent with this, a reduction in CDK1 and CDK2 expression with no change in cyclin A and B1 was observed in this cell line. Activation of caspase-2 was also detected. However, the involvement of the caspase-dependent pathway in the process of cell death induced by either BSC 3g or 3n is discarded since cell death could not be prevented by pretreatment with the pancaspase inhibitor z-VAD-fmk. Moreover, since reduced levels of p21CIP and Chk2 proteins but no change in p53 levels could be detected in MCF-7 cells after BSC 3g or 3n treatment our results suggest that BSC treated cells die from lethal mitosis. 2013-04-04T08:21:15Z 2013-04-04T08:21:15Z 2013-04-04 2012-10-24 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/10171/28202 spa info:eu-repo/semantics/openAccess application/pdf Universidad de Navarra |
| spellingShingle | Materias Investigacion::Ciencias de la vida::Bioquímica Cultivo celular Biología molecular Química orgánica Lamberto, I. (Iranzu) Palop-Cubillo, J.A. (Juan Antonio) Encío, I. (Ignacio) Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato |
| title | Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato |
| title_full | Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato |
| title_fullStr | Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato |
| title_full_unstemmed | Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato |
| title_short | Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato |
| title_sort | caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato |
| topic | Materias Investigacion::Ciencias de la vida::Bioquímica Cultivo celular Biología molecular Química orgánica |
| url | https://hdl.handle.net/10171/28202 |
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