Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells
Bacterial cyclic glucans are glucose polymers that concentrate within the periplasm of alpha-proteobacteria. These molecules are necessary to maintain the homeostasis of the cell envelope by contributing to the osmolarity of Gram negative bacteria. Here, we demonstrate that Brucella beta 1,2 cyclic...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Public Library of Science
2013
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| Online Access: | https://hdl.handle.net/10171/29415 |
| _version_ | 1793400053784641536 |
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| author | Martirosyan, A. (Anna) Perez-Gutierrez, C. (Camino) Banchereau, R. (Romain) Dutartre, H. (Hélène) Lecine, P. (Patrick) Dullaers, M. (Melissa) Mello, M. (Marielle) Pinto, S. (Suzana) Muller, A. (Alexandre) Leserman, L. (Lee) Levy, Y. (Yves) Zurawski, G. (Gerard) Zurawski, S. (Sandy) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Klechesvky, E. (Eynav) Banchereau, J. (Jacques) Oh, S. (SangKon) Gorvel, J.P. (Jean Pierre) |
| author_facet | Martirosyan, A. (Anna) Perez-Gutierrez, C. (Camino) Banchereau, R. (Romain) Dutartre, H. (Hélène) Lecine, P. (Patrick) Dullaers, M. (Melissa) Mello, M. (Marielle) Pinto, S. (Suzana) Muller, A. (Alexandre) Leserman, L. (Lee) Levy, Y. (Yves) Zurawski, G. (Gerard) Zurawski, S. (Sandy) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Klechesvky, E. (Eynav) Banchereau, J. (Jacques) Oh, S. (SangKon) Gorvel, J.P. (Jean Pierre) |
| author_sort | Martirosyan, A. (Anna) |
| collection | DSpace |
| description | Bacterial cyclic glucans are glucose polymers that concentrate within the periplasm of alpha-proteobacteria. These molecules are necessary to maintain the homeostasis of the cell envelope by contributing to the osmolarity of Gram negative bacteria. Here, we demonstrate that Brucella beta 1,2 cyclic glucans are potent activators of human and mouse dendritic cells. Dendritic cells activation by Brucella beta 1,2 cyclic glucans requires TLR4, MyD88 and TRIF, but not CD14. The Brucella cyclic glucans showed neither toxicity nor immunogenicity compared to LPS and triggered antigen-specific CD8(+) T cell responses in vivo. These cyclic glucans also enhanced antigen-specific CD4(+) and CD8(+) T cell responses including cross-presentation by different human DC subsets. Brucella beta 1,2 cyclic glucans increased the memory CD4(+) T cell responses of blood mononuclear cells exposed to recombinant fusion proteins composed of anti-CD40 antibody and antigens from both hepatitis C virus and Mycobacterium tuberculosis. Thus cyclic glucans represent a new class of adjuvants, which might contribute to the development of effective antimicrobial therapies. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29415 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-294152020-03-04T03:50:07Z Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells Martirosyan, A. (Anna) Perez-Gutierrez, C. (Camino) Banchereau, R. (Romain) Dutartre, H. (Hélène) Lecine, P. (Patrick) Dullaers, M. (Melissa) Mello, M. (Marielle) Pinto, S. (Suzana) Muller, A. (Alexandre) Leserman, L. (Lee) Levy, Y. (Yves) Zurawski, G. (Gerard) Zurawski, S. (Sandy) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Klechesvky, E. (Eynav) Banchereau, J. (Jacques) Oh, S. (SangKon) Gorvel, J.P. (Jean Pierre) Intracellular replication Virulence factor Infection Receptor Polysaccharides Lipopolysaccharides Beta-1,2-glucan Maturation Mechanism Abortus Bacterial cyclic glucans are glucose polymers that concentrate within the periplasm of alpha-proteobacteria. These molecules are necessary to maintain the homeostasis of the cell envelope by contributing to the osmolarity of Gram negative bacteria. Here, we demonstrate that Brucella beta 1,2 cyclic glucans are potent activators of human and mouse dendritic cells. Dendritic cells activation by Brucella beta 1,2 cyclic glucans requires TLR4, MyD88 and TRIF, but not CD14. The Brucella cyclic glucans showed neither toxicity nor immunogenicity compared to LPS and triggered antigen-specific CD8(+) T cell responses in vivo. These cyclic glucans also enhanced antigen-specific CD4(+) and CD8(+) T cell responses including cross-presentation by different human DC subsets. Brucella beta 1,2 cyclic glucans increased the memory CD4(+) T cell responses of blood mononuclear cells exposed to recombinant fusion proteins composed of anti-CD40 antibody and antigens from both hepatitis C virus and Mycobacterium tuberculosis. Thus cyclic glucans represent a new class of adjuvants, which might contribute to the development of effective antimicrobial therapies. 2013-06-24T11:24:19Z 2013-06-24T11:24:19Z 2012 info:eu-repo/semantics/article https://hdl.handle.net/10171/29415 eng info:eu-repo/grantAgreement/NIH/FP7/5U19AI057234-04 info:eu-repo/semantics/openAccess application/pdf Public Library of Science |
| spellingShingle | Intracellular replication Virulence factor Infection Receptor Polysaccharides Lipopolysaccharides Beta-1,2-glucan Maturation Mechanism Abortus Martirosyan, A. (Anna) Perez-Gutierrez, C. (Camino) Banchereau, R. (Romain) Dutartre, H. (Hélène) Lecine, P. (Patrick) Dullaers, M. (Melissa) Mello, M. (Marielle) Pinto, S. (Suzana) Muller, A. (Alexandre) Leserman, L. (Lee) Levy, Y. (Yves) Zurawski, G. (Gerard) Zurawski, S. (Sandy) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Klechesvky, E. (Eynav) Banchereau, J. (Jacques) Oh, S. (SangKon) Gorvel, J.P. (Jean Pierre) Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells |
| title | Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells |
| title_full | Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells |
| title_fullStr | Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells |
| title_full_unstemmed | Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells |
| title_short | Brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells |
| title_sort | brucella beta 1,2 cyclic glucan is an activator of human and mouse dendritic cells |
| topic | Intracellular replication Virulence factor Infection Receptor Polysaccharides Lipopolysaccharides Beta-1,2-glucan Maturation Mechanism Abortus |
| url | https://hdl.handle.net/10171/29415 |
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