Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats
Hapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (...
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society for Microbiology
2013
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| Online Access: | https://hdl.handle.net/10171/29443 |
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| author | Diaz-Aparicio, E. (E.) Aragon, V. (V.) Marin, C.M. (C. M.) Alonso-Urmeneta, B. (Begoña) Font, M. (M.) Moreno, E. (Edgardo) Perez-Ortiz, S. (S.) Blasco, J.M. (J. M.) Diaz, R. (Ramón) Moriyon, I. (Ignacio) |
| author_facet | Diaz-Aparicio, E. (E.) Aragon, V. (V.) Marin, C.M. (C. M.) Alonso-Urmeneta, B. (Begoña) Font, M. (M.) Moreno, E. (Edgardo) Perez-Ortiz, S. (S.) Blasco, J.M. (J. M.) Diaz, R. (Ramón) Moriyon, I. (Ignacio) |
| author_sort | Diaz-Aparicio, E. (E.) |
| collection | DSpace |
| description | Hapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (NH) of lipopolysaccharide core sugars. The polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (Brucella abortus biotype 1 [A serotype] and Brucella melitensis biotype 3 [AM serotype]), sheep (B. melitensis biotypes 1 [M serotype] and 3), and goats (B. melitensis biotype 1). The reverse radial immunodiffusion test with the NH from B. melitensis 16 M (serotype M) showed the highest sensitivity (89.6 to 97.3%), regardless of the host species and the serotype of the infecting Brucella sp. Y. enterocolitica O:9 NH (A serotype) was useful for diagnosing disease in cattle infected with B. abortus biotype 1, but not in cattle infected with B. melitensis biotype 3, sheep, or goats. The different results obtained with the serotype M and A polysaccharides and the sera from animals infected with M, A, and AM serotypes of Brucella spp. showed that in naturally infected animals, a large proportion of the antibodies are directed to or react with a previously defined common epitope(s) (J. T. Douglas and D. A. Palmer, J. Clin. Microbiol. 26:1353-1356, 1988) different from the A or M epitopes. By using the radial immunodiffusion test with B. melitensis 16M NH, it was possible to differentiate infected from vaccinated cattle, sheep, and goats with a sensitivity and specificity similar to that of the complement fixation test. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29443 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-294432020-03-03T22:53:36Z Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats Diaz-Aparicio, E. (E.) Aragon, V. (V.) Marin, C.M. (C. M.) Alonso-Urmeneta, B. (Begoña) Font, M. (M.) Moreno, E. (Edgardo) Perez-Ortiz, S. (S.) Blasco, J.M. (J. M.) Diaz, R. (Ramón) Moriyon, I. (Ignacio) Radial immunodiffusion test Linked immunosorbent-assay Agar-gel immunodifussion Monoclonal-antibodies Bovine brucellosis Enzyme-immunoassay Native hapten Abortus Lipopolysaccharides Melitensis Hapten polysaccharides of Brucella smooth M and A serotypes were prepared from Brucella sp. and Yersinia enterocolitica O:9 by previously described hydrolytic (O chain) or nonhydrolytic (native hapten [NH]) procedures. The purified polysaccharides differed only in the presence (O chain) or absence (NH) of lipopolysaccharide core sugars. The polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (Brucella abortus biotype 1 [A serotype] and Brucella melitensis biotype 3 [AM serotype]), sheep (B. melitensis biotypes 1 [M serotype] and 3), and goats (B. melitensis biotype 1). The reverse radial immunodiffusion test with the NH from B. melitensis 16 M (serotype M) showed the highest sensitivity (89.6 to 97.3%), regardless of the host species and the serotype of the infecting Brucella sp. Y. enterocolitica O:9 NH (A serotype) was useful for diagnosing disease in cattle infected with B. abortus biotype 1, but not in cattle infected with B. melitensis biotype 3, sheep, or goats. The different results obtained with the serotype M and A polysaccharides and the sera from animals infected with M, A, and AM serotypes of Brucella spp. showed that in naturally infected animals, a large proportion of the antibodies are directed to or react with a previously defined common epitope(s) (J. T. Douglas and D. A. Palmer, J. Clin. Microbiol. 26:1353-1356, 1988) different from the A or M epitopes. By using the radial immunodiffusion test with B. melitensis 16M NH, it was possible to differentiate infected from vaccinated cattle, sheep, and goats with a sensitivity and specificity similar to that of the complement fixation test. 2013-07-01T08:18:47Z 2013-07-01T08:18:47Z 1993 info:eu-repo/semantics/article https://hdl.handle.net/10171/29443 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Radial immunodiffusion test Linked immunosorbent-assay Agar-gel immunodifussion Monoclonal-antibodies Bovine brucellosis Enzyme-immunoassay Native hapten Abortus Lipopolysaccharides Melitensis Diaz-Aparicio, E. (E.) Aragon, V. (V.) Marin, C.M. (C. M.) Alonso-Urmeneta, B. (Begoña) Font, M. (M.) Moreno, E. (Edgardo) Perez-Ortiz, S. (S.) Blasco, J.M. (J. M.) Diaz, R. (Ramón) Moriyon, I. (Ignacio) Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title | Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_full | Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_fullStr | Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_full_unstemmed | Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_short | Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| title_sort | comparative analysis of brucella serotype a and m and yersinia enterocolitica o:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats |
| topic | Radial immunodiffusion test Linked immunosorbent-assay Agar-gel immunodifussion Monoclonal-antibodies Bovine brucellosis Enzyme-immunoassay Native hapten Abortus Lipopolysaccharides Melitensis |
| url | https://hdl.handle.net/10171/29443 |
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