Generation of the Brucella melitensis ORFeome version 1.1.
The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecula...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Cold Spring Habor Laboratory Press
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/29445 |
| _version_ | 1793400032785858560 |
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| author | Dricot, A. (Amélie) Rual, J. J. (Jean François) Lamesch, P. (Philippe) Bertin, N. (Nicolas) Dupuy, D. (Denis) Hao, T. (Tong) Lambert, C. (Christophe) Hallez, R. (Régis) Delroisse, J.M. (Jean Marc) Vandenhaute, J. (Jean) Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Garcia-Lobo, J.M. (Juan María) Sangari, F.J. (Félix Javier) MacMillan, A.P. (Alastair P.) Cutler, S.J. (Sally J.) Whatmore, A.M. (Adrian M.) Bozak, S. (Stephanie) Sequerra, R. (Reynaldo) Doucettte-Stamm, L. (Lynn) Vidal, M. (Marc) Hill, D.E. (David E.) Letesson, J.J. (Jean Jacques) Bolle, X. (Xavier) de |
| author_facet | Dricot, A. (Amélie) Rual, J. J. (Jean François) Lamesch, P. (Philippe) Bertin, N. (Nicolas) Dupuy, D. (Denis) Hao, T. (Tong) Lambert, C. (Christophe) Hallez, R. (Régis) Delroisse, J.M. (Jean Marc) Vandenhaute, J. (Jean) Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Garcia-Lobo, J.M. (Juan María) Sangari, F.J. (Félix Javier) MacMillan, A.P. (Alastair P.) Cutler, S.J. (Sally J.) Whatmore, A.M. (Adrian M.) Bozak, S. (Stephanie) Sequerra, R. (Reynaldo) Doucettte-Stamm, L. (Lynn) Vidal, M. (Marc) Hill, D.E. (David E.) Letesson, J.J. (Jean Jacques) Bolle, X. (Xavier) de |
| author_sort | Dricot, A. (Amélie) |
| collection | DSpace |
| description | The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of 3091 Gateway Entry clones, each containing a defined ORF. This first version of the Brucella ORFeome (v1.1) provides the coding sequences in a user-friendly format amenable to high-throughput functional genomic and proteomic experiments, as the ORFs are conveniently transferable from the Entry clones to various Expression vectors by recombinational cloning. The cloning of the Brucella ORFeome v1.1 should help to provide a better understanding of the molecular mechanisms of virulence, including the identification of bacterial protein-protein interactions, but also interactions between bacterial effectors and their host's targets. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29445 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | Cold Spring Habor Laboratory Press |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-294452020-03-04T02:25:02Z Generation of the Brucella melitensis ORFeome version 1.1. Dricot, A. (Amélie) Rual, J. J. (Jean François) Lamesch, P. (Philippe) Bertin, N. (Nicolas) Dupuy, D. (Denis) Hao, T. (Tong) Lambert, C. (Christophe) Hallez, R. (Régis) Delroisse, J.M. (Jean Marc) Vandenhaute, J. (Jean) Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Garcia-Lobo, J.M. (Juan María) Sangari, F.J. (Félix Javier) MacMillan, A.P. (Alastair P.) Cutler, S.J. (Sally J.) Whatmore, A.M. (Adrian M.) Bozak, S. (Stephanie) Sequerra, R. (Reynaldo) Doucettte-Stamm, L. (Lynn) Vidal, M. (Marc) Hill, D.E. (David E.) Letesson, J.J. (Jean Jacques) Bolle, X. (Xavier) de Bacterial proteins genetics Brucella melitensis genetics Genome, bacterial Open reading frames physiology The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of 3091 Gateway Entry clones, each containing a defined ORF. This first version of the Brucella ORFeome (v1.1) provides the coding sequences in a user-friendly format amenable to high-throughput functional genomic and proteomic experiments, as the ORFs are conveniently transferable from the Entry clones to various Expression vectors by recombinational cloning. The cloning of the Brucella ORFeome v1.1 should help to provide a better understanding of the molecular mechanisms of virulence, including the identification of bacterial protein-protein interactions, but also interactions between bacterial effectors and their host's targets. 2013-07-01T10:14:05Z 2013-07-01T10:14:05Z 2004 info:eu-repo/semantics/article https://hdl.handle.net/10171/29445 eng info:eu-repo/semantics/openAccess application/pdf Cold Spring Habor Laboratory Press |
| spellingShingle | Bacterial proteins genetics Brucella melitensis genetics Genome, bacterial Open reading frames physiology Dricot, A. (Amélie) Rual, J. J. (Jean François) Lamesch, P. (Philippe) Bertin, N. (Nicolas) Dupuy, D. (Denis) Hao, T. (Tong) Lambert, C. (Christophe) Hallez, R. (Régis) Delroisse, J.M. (Jean Marc) Vandenhaute, J. (Jean) Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Garcia-Lobo, J.M. (Juan María) Sangari, F.J. (Félix Javier) MacMillan, A.P. (Alastair P.) Cutler, S.J. (Sally J.) Whatmore, A.M. (Adrian M.) Bozak, S. (Stephanie) Sequerra, R. (Reynaldo) Doucettte-Stamm, L. (Lynn) Vidal, M. (Marc) Hill, D.E. (David E.) Letesson, J.J. (Jean Jacques) Bolle, X. (Xavier) de Generation of the Brucella melitensis ORFeome version 1.1. |
| title | Generation of the Brucella melitensis ORFeome version 1.1. |
| title_full | Generation of the Brucella melitensis ORFeome version 1.1. |
| title_fullStr | Generation of the Brucella melitensis ORFeome version 1.1. |
| title_full_unstemmed | Generation of the Brucella melitensis ORFeome version 1.1. |
| title_short | Generation of the Brucella melitensis ORFeome version 1.1. |
| title_sort | generation of the brucella melitensis orfeome version 1.1. |
| topic | Bacterial proteins genetics Brucella melitensis genetics Genome, bacterial Open reading frames physiology |
| url | https://hdl.handle.net/10171/29445 |
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