Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures
The detection of Brucella bacteremia by subculture does not always correlate with a positive signal in the BACTEC NR730 nonradiometric system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.). The effect of the inoculum size, pH, sodium polyanetholesulfonate, carbon sources (i-erythritol...
| Main Authors: | , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | eng |
| Published: |
American Society for Microbiology
2013
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/29451 |
| _version_ | 1793400101934202880 |
|---|---|
| author | Gamazo, C. (Carlos) Vitas, A.I. (Ana Isabel) Lopez-Goñi, I. (Ignacio) Diaz, R. (Ramón) Moriyon, I. (Ignacio) |
| author_facet | Gamazo, C. (Carlos) Vitas, A.I. (Ana Isabel) Lopez-Goñi, I. (Ignacio) Diaz, R. (Ramón) Moriyon, I. (Ignacio) |
| author_sort | Gamazo, C. (Carlos) |
| collection | DSpace |
| description | The detection of Brucella bacteremia by subculture does not always correlate with a positive signal in the BACTEC NR730 nonradiometric system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.). The effect of the inoculum size, pH, sodium polyanetholesulfonate, carbon sources (i-erythritol, sodium pyruvate, monosodium glutamate, D-glucose, and L-alanine), and urea in the release of CO2 was evaluated by using the reference strain Brucella melitensis 16M. In standard NR6 vials with or without blood, inocula 5 to 10 times larger (at least 265 CFU per vial) than those usually found in the blood of patients with brucellosis were necessary to produce a positive growth value (GV) in 4 days or less, and similar results were obtained with vials supplemented with the substrates listed above. GVs were consistently lower in vials with sodium polyanetholesulfonate than in vials without this agent. Vials with no blood inoculated with 265 CFU per vial showed turbidity 1 day before GVs became positive, proving that the major limiting detection factor was the low level of release of CO2 and not an inadequate growth medium. In NR6 vials buffered to pH 6.2, GVs became positive faster and were higher than those in standard vials. NR6 vials at pH 6.2 with 0.3% sodium pyruvate yielded a positive GV in the first day of bacterial turbidity. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29451 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-294512020-03-03T20:45:08Z Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures Gamazo, C. (Carlos) Vitas, A.I. (Ana Isabel) Lopez-Goñi, I. (Ignacio) Diaz, R. (Ramón) Moriyon, I. (Ignacio) Bacteremia diagnosis Bacteriological techniques instrumentation Brucella melitensis isolation and purification Brucellosis diagnosis The detection of Brucella bacteremia by subculture does not always correlate with a positive signal in the BACTEC NR730 nonradiometric system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.). The effect of the inoculum size, pH, sodium polyanetholesulfonate, carbon sources (i-erythritol, sodium pyruvate, monosodium glutamate, D-glucose, and L-alanine), and urea in the release of CO2 was evaluated by using the reference strain Brucella melitensis 16M. In standard NR6 vials with or without blood, inocula 5 to 10 times larger (at least 265 CFU per vial) than those usually found in the blood of patients with brucellosis were necessary to produce a positive growth value (GV) in 4 days or less, and similar results were obtained with vials supplemented with the substrates listed above. GVs were consistently lower in vials with sodium polyanetholesulfonate than in vials without this agent. Vials with no blood inoculated with 265 CFU per vial showed turbidity 1 day before GVs became positive, proving that the major limiting detection factor was the low level of release of CO2 and not an inadequate growth medium. In NR6 vials buffered to pH 6.2, GVs became positive faster and were higher than those in standard vials. NR6 vials at pH 6.2 with 0.3% sodium pyruvate yielded a positive GV in the first day of bacterial turbidity. 2013-07-02T07:43:40Z 2013-07-02T07:43:40Z 1993 info:eu-repo/semantics/article https://hdl.handle.net/10171/29451 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Bacteremia diagnosis Bacteriological techniques instrumentation Brucella melitensis isolation and purification Brucellosis diagnosis Gamazo, C. (Carlos) Vitas, A.I. (Ana Isabel) Lopez-Goñi, I. (Ignacio) Diaz, R. (Ramón) Moriyon, I. (Ignacio) Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures |
| title | Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures |
| title_full | Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures |
| title_fullStr | Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures |
| title_full_unstemmed | Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures |
| title_short | Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures |
| title_sort | factors affecting detection of brucella melitensis by bactec nr730, a nonradiometric system for hemocultures |
| topic | Bacteremia diagnosis Bacteriological techniques instrumentation Brucella melitensis isolation and purification Brucellosis diagnosis |
| url | https://hdl.handle.net/10171/29451 |
| work_keys_str_mv | AT gamazoccarlos factorsaffectingdetectionofbrucellamelitensisbybactecnr730anonradiometricsystemforhemocultures AT vitasaianaisabel factorsaffectingdetectionofbrucellamelitensisbybactecnr730anonradiometricsystemforhemocultures AT lopezgoniiignacio factorsaffectingdetectionofbrucellamelitensisbybactecnr730anonradiometricsystemforhemocultures AT diazrramon factorsaffectingdetectionofbrucellamelitensisbybactecnr730anonradiometricsystemforhemocultures AT moriyoniignacio factorsaffectingdetectionofbrucellamelitensisbybactecnr730anonradiometricsystemforhemocultures |