Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore
Brucella abortus grown in low-iron medium or in the presence of iron chelators [ethylenediamine-di(o-hydroxyphenylacetic acid) and 2,2-dipyridyl] showed reduced cell yields and released a material positive in chemical and biological assays for catechols. This material was purified from culture fluid...
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society for Microbiology
2013
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| Online Access: | https://hdl.handle.net/10171/29508 |
| _version_ | 1793400054868869120 |
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| author | Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Neilands, J.B. (J. B.) |
| author_facet | Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Neilands, J.B. (J. B.) |
| author_sort | Lopez-Goñi, I. (Ignacio) |
| collection | DSpace |
| description | Brucella abortus grown in low-iron medium or in the presence of iron chelators [ethylenediamine-di(o-hydroxyphenylacetic acid) and 2,2-dipyridyl] showed reduced cell yields and released a material positive in chemical and biological assays for catechols. This material was purified from culture fluids of B. abortus 2308 by chromatography on agarose-iminodiacetic acid-Fe3+ and identified as 2,3-dihydroxybenzoic acid (2,3-DHBA) by thin-layer chromatography, paper electrophoresis, and UV-visible nuclear magnetic resonance and mass spectroscopy. No other major catechols were observed at different stages of growth, and 2,3-DHBA was also produced upon iron limitation by representative strains of B. abortus biotypes 1, 5, 6, and 9. Both synthetic 2,3-DHBA and the natural catechol relieved the growth inhibition of B. abortus 2308 by ethylenediamine-di(o-hydroxyphenylacetic acid), and 2,3-DHBA promoted 55Fe uptake by B. abortus 2308 by an energy-dependent mechanism. Two other monocatechols tested, 2,3-dihydroxybenzoyl-Ser and 2,3-dihydroxybenzoyl-Gly, also promoted 55Fe uptake. More complex catechol siderophores (agrobactin and enterobactin), hydroxamate siderophores (aerobactin, ferrichrome, and deferriferrioxamine mesylate [Desferal]), and an EDTA-related siderophore (rhizobactin) failed to mediate 55Fe uptake. B. abortus cells grown in low-iron medium or in medium with iron had similar rates of iron uptake when supplied with 55Fe-2,3-DHBA, and the release of 2,3-DHBA under iron starvation was not associated with the expression of new outer membrane proteins. These results suggest an uptake system in which only the synthesis of the siderophore is regulated by the iron available for growth. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29508 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-295082020-03-03T20:43:21Z Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Neilands, J.B. (J. B.) Brucella abortus metabolism Hydroxybenzoates metabolism Siderophores physiology Brucella abortus grown in low-iron medium or in the presence of iron chelators [ethylenediamine-di(o-hydroxyphenylacetic acid) and 2,2-dipyridyl] showed reduced cell yields and released a material positive in chemical and biological assays for catechols. This material was purified from culture fluids of B. abortus 2308 by chromatography on agarose-iminodiacetic acid-Fe3+ and identified as 2,3-dihydroxybenzoic acid (2,3-DHBA) by thin-layer chromatography, paper electrophoresis, and UV-visible nuclear magnetic resonance and mass spectroscopy. No other major catechols were observed at different stages of growth, and 2,3-DHBA was also produced upon iron limitation by representative strains of B. abortus biotypes 1, 5, 6, and 9. Both synthetic 2,3-DHBA and the natural catechol relieved the growth inhibition of B. abortus 2308 by ethylenediamine-di(o-hydroxyphenylacetic acid), and 2,3-DHBA promoted 55Fe uptake by B. abortus 2308 by an energy-dependent mechanism. Two other monocatechols tested, 2,3-dihydroxybenzoyl-Ser and 2,3-dihydroxybenzoyl-Gly, also promoted 55Fe uptake. More complex catechol siderophores (agrobactin and enterobactin), hydroxamate siderophores (aerobactin, ferrichrome, and deferriferrioxamine mesylate [Desferal]), and an EDTA-related siderophore (rhizobactin) failed to mediate 55Fe uptake. B. abortus cells grown in low-iron medium or in medium with iron had similar rates of iron uptake when supplied with 55Fe-2,3-DHBA, and the release of 2,3-DHBA under iron starvation was not associated with the expression of new outer membrane proteins. These results suggest an uptake system in which only the synthesis of the siderophore is regulated by the iron available for growth. 2013-07-18T11:35:59Z 2013-07-18T11:35:59Z 1992 info:eu-repo/semantics/article https://hdl.handle.net/10171/29508 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Brucella abortus metabolism Hydroxybenzoates metabolism Siderophores physiology Lopez-Goñi, I. (Ignacio) Moriyon, I. (Ignacio) Neilands, J.B. (J. B.) Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore |
| title | Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore |
| title_full | Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore |
| title_fullStr | Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore |
| title_full_unstemmed | Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore |
| title_short | Identification of 2,3-dihydroxybenzoic acid as a Brucella abortus siderophore |
| title_sort | identification of 2,3-dihydroxybenzoic acid as a brucella abortus siderophore |
| topic | Brucella abortus metabolism Hydroxybenzoates metabolism Siderophores physiology |
| url | https://hdl.handle.net/10171/29508 |
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