Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis
Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most s...
| Main Authors: | , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society for Microbiology
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/29516 |
| _version_ | 1793400009459236864 |
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| author | Marin, C.M. (C. M.) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Diaz, R. (Ramón) Blasco, J.M. (J. M.) |
| author_facet | Marin, C.M. (C. M.) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Diaz, R. (Ramón) Blasco, J.M. (J. M.) |
| author_sort | Marin, C.M. (C. M.) |
| collection | DSpace |
| description | Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most specific tests were AGID-NH and competitive ELISA. We show that RB followed by AGID-NH is a simple and effective system for diagnosing sheep brucellosis. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29516 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-295162020-03-04T00:23:16Z Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis Marin, C.M. (C. M.) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Diaz, R. (Ramón) Blasco, J.M. (J. M.) Brucella melitensis Brucellosis diagnosis Enzyme-Linked immunosorbent assay methods Haptens analysis Sheep diseases diagnosis Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most specific tests were AGID-NH and competitive ELISA. We show that RB followed by AGID-NH is a simple and effective system for diagnosing sheep brucellosis. 2013-07-19T08:39:39Z 2013-07-19T08:39:39Z 1999 info:eu-repo/semantics/article https://hdl.handle.net/10171/29516 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Brucella melitensis Brucellosis diagnosis Enzyme-Linked immunosorbent assay methods Haptens analysis Sheep diseases diagnosis Marin, C.M. (C. M.) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Diaz, R. (Ramón) Blasco, J.M. (J. M.) Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis |
| title | Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis |
| title_full | Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis |
| title_fullStr | Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis |
| title_full_unstemmed | Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis |
| title_short | Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis |
| title_sort | performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis |
| topic | Brucella melitensis Brucellosis diagnosis Enzyme-Linked immunosorbent assay methods Haptens analysis Sheep diseases diagnosis |
| url | https://hdl.handle.net/10171/29516 |
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