The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides
The actions of polymyxin B, rabbit polymorphonuclear lysosome extracts, 14 polycationic peptides (including defensin NP-2, cecropin P1, lactoferricin B, and active peptides from cationic protein 18 and bactenecin), EDTA, and Tris on Brucella spp. were studied, with other gram-negative bacteria as co...
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society for Microbiology
2013
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| Online Access: | https://hdl.handle.net/10171/29518 |
| _version_ | 1793400103330906112 |
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| author | Martinez-de-Tejada, G. (Guillermo) Pizarro-Cerda, J. (Javier) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) |
| author_facet | Martinez-de-Tejada, G. (Guillermo) Pizarro-Cerda, J. (Javier) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) |
| author_sort | Martinez-de-Tejada, G. (Guillermo) |
| collection | DSpace |
| description | The actions of polymyxin B, rabbit polymorphonuclear lysosome extracts, 14 polycationic peptides (including defensin NP-2, cecropin P1, lactoferricin B, and active peptides from cationic protein 18 and bactenecin), EDTA, and Tris on Brucella spp. were studied, with other gram-negative bacteria as controls. Brucella spp. were comparatively resistant to all of the agents listed above and bound less polymyxin B, and their outer membranes (OMs) were neither morphologically altered nor permeabilized to lysozyme by polymyxin B concentrations, although both effects were observed for controls. EDTA and peptides increased or accelerated the partition of the hydrophobic probe N-phenyl-naphthylamine into Escherichia coli and Haemophilus influenzae OMs but had no effect on Brucella OMs. Since Brucella and H. influenzae OMs are permeable to hydrophobic compounds (G. Martínez de Tejada and I. Moriyón, J. Bacteriol. 175:5273-5275, 1993), the results show that such unusual permeability is not necessarily related to resistance to polycations. Although rough (R) B. abortus and B. ovis were more resistant than the controls were, there were qualitative and quantitative differences with smooth (S) brucellae; this may explain known host range and virulence differences. Brucella S-lipopolysaccharides (LPSs) had reduced affinities for polycations, and insertion of Brucella and Salmonella montevideo S-LPSs into the OM of a Brucella R-LPS mutant increased and decreased, respectively, its resistance to cationic peptides. The results show that the core lipid A of Brucella LPS plays a major role in polycation resistance and that O-chain density also contributes significantly. It is proposed that the features described above contribute to Brucella resistance to the oxygen-independent systems of phagocytes. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29518 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-295182020-03-03T23:46:48Z The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides Martinez-de-Tejada, G. (Guillermo) Pizarro-Cerda, J. (Javier) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) Brucella drug effects Defensins Proteins phamarcology Xenopus proteins The actions of polymyxin B, rabbit polymorphonuclear lysosome extracts, 14 polycationic peptides (including defensin NP-2, cecropin P1, lactoferricin B, and active peptides from cationic protein 18 and bactenecin), EDTA, and Tris on Brucella spp. were studied, with other gram-negative bacteria as controls. Brucella spp. were comparatively resistant to all of the agents listed above and bound less polymyxin B, and their outer membranes (OMs) were neither morphologically altered nor permeabilized to lysozyme by polymyxin B concentrations, although both effects were observed for controls. EDTA and peptides increased or accelerated the partition of the hydrophobic probe N-phenyl-naphthylamine into Escherichia coli and Haemophilus influenzae OMs but had no effect on Brucella OMs. Since Brucella and H. influenzae OMs are permeable to hydrophobic compounds (G. Martínez de Tejada and I. Moriyón, J. Bacteriol. 175:5273-5275, 1993), the results show that such unusual permeability is not necessarily related to resistance to polycations. Although rough (R) B. abortus and B. ovis were more resistant than the controls were, there were qualitative and quantitative differences with smooth (S) brucellae; this may explain known host range and virulence differences. Brucella S-lipopolysaccharides (LPSs) had reduced affinities for polycations, and insertion of Brucella and Salmonella montevideo S-LPSs into the OM of a Brucella R-LPS mutant increased and decreased, respectively, its resistance to cationic peptides. The results show that the core lipid A of Brucella LPS plays a major role in polycation resistance and that O-chain density also contributes significantly. It is proposed that the features described above contribute to Brucella resistance to the oxygen-independent systems of phagocytes. 2013-07-19T09:05:47Z 2013-07-19T09:05:47Z 1995 info:eu-repo/semantics/article https://hdl.handle.net/10171/29518 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Brucella drug effects Defensins Proteins phamarcology Xenopus proteins Martinez-de-Tejada, G. (Guillermo) Pizarro-Cerda, J. (Javier) Moreno, E. (Edgardo) Moriyon, I. (Ignacio) The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides |
| title | The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides |
| title_full | The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides |
| title_fullStr | The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides |
| title_full_unstemmed | The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides |
| title_short | The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides |
| title_sort | outer membranes of brucella spp. are resistant to bactericidal cationic peptides |
| topic | Brucella drug effects Defensins Proteins phamarcology Xenopus proteins |
| url | https://hdl.handle.net/10171/29518 |
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