Characterization of a native polysaccharide hapten from Brucella melitensis

The 13C nuclear magnetic resonance spectrum of Brucella melitensis native polysaccharide hapten proved to be very similar to that generated by the O-specific chain (PS) isolated from B. melitensis lipopolysaccharide; that is, to a linear polymer in which the repeating unit is composed of five N-form...

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Bibliographic Details
Main Authors: Moreno, E. (Edgardo), Mayer, H. (Hubert), Moriyon, I. (Ignacio)
Format: info:eu-repo/semantics/article
Language:eng
Published: American Society for Microbiology 2013
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Online Access:https://hdl.handle.net/10171/29522
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Summary:The 13C nuclear magnetic resonance spectrum of Brucella melitensis native polysaccharide hapten proved to be very similar to that generated by the O-specific chain (PS) isolated from B. melitensis lipopolysaccharide; that is, to a linear polymer in which the repeating unit is composed of five N-formylperosaminyl residues, one of them being substituted at position C-3 and the other four at position C-2. The serological analysis suggests that the so-called A determinant is present solely in Brucella abortus PS, the M determinant is only in B. melitensis PS, and the extensive cross-reaction observed is due to a determinant shared by both polysaccharides.