Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR
Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temp...
| Main Authors: | , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | eng |
| Published: |
American Society for Microbiology
2013
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/29637 |
| _version_ | 1793399987657244672 |
|---|---|
| author | Romero, C. (Conchi) Lopez-Goñi, I. (Ignacio) |
| author_facet | Romero, C. (Conchi) Lopez-Goñi, I. (Ignacio) |
| author_sort | Romero, C. (Conchi) |
| collection | DSpace |
| description | Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29637 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-296372020-03-03T20:47:38Z Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR Romero, C. (Conchi) Lopez-Goñi, I. (Ignacio) Blood RNA Biotechnology and applied microbiology Microbiology Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk. 2013-08-30T11:19:01Z 2013-08-30T11:19:01Z 1999 info:eu-repo/semantics/article https://hdl.handle.net/10171/29637 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Blood RNA Biotechnology and applied microbiology Microbiology Romero, C. (Conchi) Lopez-Goñi, I. (Ignacio) Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR |
| title | Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR |
| title_full | Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR |
| title_fullStr | Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR |
| title_full_unstemmed | Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR |
| title_short | Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR |
| title_sort | improved method for purification of bacterial dna from bovine milk for detection of brucella spp. by pcr |
| topic | Blood RNA Biotechnology and applied microbiology Microbiology |
| url | https://hdl.handle.net/10171/29637 |
| work_keys_str_mv | AT romerocconchi improvedmethodforpurificationofbacterialdnafrombovinemilkfordetectionofbrucellasppbypcr AT lopezgoniiignacio improvedmethodforpurificationofbacterialdnafrombovinemilkfordetectionofbrucellasppbypcr |