Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle
A study was performed to evaluate the previously described PCR (C. Romero, C. Gamazo, M. Pardo, and I. López-Goñi, J. Clin. Microbiol. 33:615-617, 1995) for the diagnosis of brucellosis in dairy cattle. Milk samples from 56 Brucella milk culture-positive cattle and from 37 cattle from Brucella-free...
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society for Microbiology
2013
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| Online Access: | https://hdl.handle.net/10171/29638 |
| _version_ | 1793400103812202496 |
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| author | Romero, C. (Conchi) Pardo, M. (Marisa) Grillo, M.J. (María Jesús) Diaz, R. (Ramón) Blasco, J.M. (J. M.) Lopez-Goñi, I. (Ignacio) |
| author_facet | Romero, C. (Conchi) Pardo, M. (Marisa) Grillo, M.J. (María Jesús) Diaz, R. (Ramón) Blasco, J.M. (J. M.) Lopez-Goñi, I. (Ignacio) |
| author_sort | Romero, C. (Conchi) |
| collection | DSpace |
| description | A study was performed to evaluate the previously described PCR (C. Romero, C. Gamazo, M. Pardo, and I. López-Goñi, J. Clin. Microbiol. 33:615-617, 1995) for the diagnosis of brucellosis in dairy cattle. Milk samples from 56 Brucella milk culture-positive cattle and from 37 cattle from Brucella-free herds were examined for Brucella DNA by PCR and for specific antibodies by an indirect enzyme-linked immunosorbent assay (ELISA). The specificities of both tests were 100% when testing the milk samples from Brucella-free cattle. The milk samples from 49 infected cattle were positive by PCR (87.5% sensitivity), and 55 were positive by ELISA (98.2% sensitivity). A PCR-positive sample was negative by ELISA, and 7 ELISA-positive samples were PCR negative, yielding an observed proportion of agreement of 0.91 for the two tests. Although the results suggest that ELISA is a better screening test than PCR, the combined sensitivity of the two assays was 100%, and their simultaneous application could be more useful than one test alone for a rapid screening of brucellosis in dairy cattle. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29638 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-296382020-03-03T22:31:57Z Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle Romero, C. (Conchi) Pardo, M. (Marisa) Grillo, M.J. (María Jesús) Diaz, R. (Ramón) Blasco, J.M. (J. M.) Lopez-Goñi, I. (Ignacio) Brucella genetics Brucella immunology Brucellosis, Bovine diagnosis Enzyme-Linked Immunosorbent Assay methods Milk microbiology Polymerase Chain Reaction methods Female A study was performed to evaluate the previously described PCR (C. Romero, C. Gamazo, M. Pardo, and I. López-Goñi, J. Clin. Microbiol. 33:615-617, 1995) for the diagnosis of brucellosis in dairy cattle. Milk samples from 56 Brucella milk culture-positive cattle and from 37 cattle from Brucella-free herds were examined for Brucella DNA by PCR and for specific antibodies by an indirect enzyme-linked immunosorbent assay (ELISA). The specificities of both tests were 100% when testing the milk samples from Brucella-free cattle. The milk samples from 49 infected cattle were positive by PCR (87.5% sensitivity), and 55 were positive by ELISA (98.2% sensitivity). A PCR-positive sample was negative by ELISA, and 7 ELISA-positive samples were PCR negative, yielding an observed proportion of agreement of 0.91 for the two tests. Although the results suggest that ELISA is a better screening test than PCR, the combined sensitivity of the two assays was 100%, and their simultaneous application could be more useful than one test alone for a rapid screening of brucellosis in dairy cattle. 2013-08-30T11:33:08Z 2013-08-30T11:33:08Z 1995 info:eu-repo/semantics/article https://hdl.handle.net/10171/29638 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Brucella genetics Brucella immunology Brucellosis, Bovine diagnosis Enzyme-Linked Immunosorbent Assay methods Milk microbiology Polymerase Chain Reaction methods Female Romero, C. (Conchi) Pardo, M. (Marisa) Grillo, M.J. (María Jesús) Diaz, R. (Ramón) Blasco, J.M. (J. M.) Lopez-Goñi, I. (Ignacio) Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle |
| title | Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle |
| title_full | Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle |
| title_fullStr | Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle |
| title_full_unstemmed | Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle |
| title_short | Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle |
| title_sort | evaluation of pcr and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle |
| topic | Brucella genetics Brucella immunology Brucellosis, Bovine diagnosis Enzyme-Linked Immunosorbent Assay methods Milk microbiology Polymerase Chain Reaction methods Female |
| url | https://hdl.handle.net/10171/29638 |
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