Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry
The specificity and sensitivity of indirect ELISA, based on the use of four different antigenic extracts obtained from a clinical isolate of Salmonella enteritidis, were compared with those obtained with the gm-flagellin based ELISA (IDEXX). A total of 116 serum samples from salmonellae free, natura...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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BioMed Central
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/29644 |
| _version_ | 1793400151447961600 |
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| author | Solano, C. (Cristina) Galindo, J. (Julia) Sesma, B. (Begoña) Alvarez, M. (Miguel) Solsona, M.J. (María J.) Gamazo, C. (Carlos) |
| author_facet | Solano, C. (Cristina) Galindo, J. (Julia) Sesma, B. (Begoña) Alvarez, M. (Miguel) Solsona, M.J. (María J.) Gamazo, C. (Carlos) |
| author_sort | Solano, C. (Cristina) |
| collection | DSpace |
| description | The specificity and sensitivity of indirect ELISA, based on the use of four different antigenic extracts obtained from a clinical isolate of Salmonella enteritidis, were compared with those obtained with the gm-flagellin based ELISA (IDEXX). A total of 116 serum samples from salmonellae free, naturally infected and vaccinated hens were studied. The results showed that the indirect ELISA, based on lipopolysaccharide (LPS), O-polysaccharide (PS) or membrane sediment (SD) antigens, enable the identification of a greater number of infected birds and discriminated field antibody responses from vaccinal ones better than the commercial IDEXX test. The indirect ELISA that used a O-polysaccharide rich fraction (PS) proved to be the most specific and sensitive test, suggesting that this indirect ELISA could be used to confirm IDEXX results, especially when the differentiation between vaccinated and infected poultry is required. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29644 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-296442020-03-03T22:16:51Z Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry Solano, C. (Cristina) Galindo, J. (Julia) Sesma, B. (Begoña) Alvarez, M. (Miguel) Solsona, M.J. (María J.) Gamazo, C. (Carlos) Salmonella Serological diagnosis Poultry Elisa Chicken flocks Laying hens Egg-yolk Antibodies Test Veterinary sciences The specificity and sensitivity of indirect ELISA, based on the use of four different antigenic extracts obtained from a clinical isolate of Salmonella enteritidis, were compared with those obtained with the gm-flagellin based ELISA (IDEXX). A total of 116 serum samples from salmonellae free, naturally infected and vaccinated hens were studied. The results showed that the indirect ELISA, based on lipopolysaccharide (LPS), O-polysaccharide (PS) or membrane sediment (SD) antigens, enable the identification of a greater number of infected birds and discriminated field antibody responses from vaccinal ones better than the commercial IDEXX test. The indirect ELISA that used a O-polysaccharide rich fraction (PS) proved to be the most specific and sensitive test, suggesting that this indirect ELISA could be used to confirm IDEXX results, especially when the differentiation between vaccinated and infected poultry is required. 2013-09-02T09:52:32Z 2013-09-02T09:52:32Z 2000 info:eu-repo/semantics/article https://hdl.handle.net/10171/29644 eng info:eu-repo/semantics/openAccess application/pdf BioMed Central |
| spellingShingle | Salmonella Serological diagnosis Poultry Elisa Chicken flocks Laying hens Egg-yolk Antibodies Test Veterinary sciences Solano, C. (Cristina) Galindo, J. (Julia) Sesma, B. (Begoña) Alvarez, M. (Miguel) Solsona, M.J. (María J.) Gamazo, C. (Carlos) Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry |
| title | Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry |
| title_full | Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry |
| title_fullStr | Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry |
| title_full_unstemmed | Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry |
| title_short | Enzyme-linked immunosorbent assay with a Salmonella enteritidis antigen for differentiating infected from vaccinated poultry |
| title_sort | enzyme-linked immunosorbent assay with a salmonella enteritidis antigen for differentiating infected from vaccinated poultry |
| topic | Salmonella Serological diagnosis Poultry Elisa Chicken flocks Laying hens Egg-yolk Antibodies Test Veterinary sciences |
| url | https://hdl.handle.net/10171/29644 |
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