Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test
The objective of this study was the in vitro differentiation of isolates of Salmonella enteritidis whose virulences differed in a chick model. A total of 14 strains of S. enteritidis were isolated from either the environment, dairy products, or infected patients. The isolates could be divided into t...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society for Microbiology
2013
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| Online Access: | https://hdl.handle.net/10171/29645 |
| _version_ | 1793400055349116928 |
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| author | Solano, C. (Cristina) Sesma, B. (Begoña) Alvarez, M. (Miguel) Humphrey, T.J. (T. J.) Thorns, C.J. (C. J.) Gamazo, C. (Carlos) |
| author_facet | Solano, C. (Cristina) Sesma, B. (Begoña) Alvarez, M. (Miguel) Humphrey, T.J. (T. J.) Thorns, C.J. (C. J.) Gamazo, C. (Carlos) |
| author_sort | Solano, C. (Cristina) |
| collection | DSpace |
| description | The objective of this study was the in vitro differentiation of isolates of Salmonella enteritidis whose virulences differed in a chick model. A total of 14 strains of S. enteritidis were isolated from either the environment, dairy products, or infected patients. The isolates could be divided into two groups on the basis of their virulence (50% lethal dose) in chickens infected intraperitoneally. When the strains were incubated in adherence test medium (Spanish patent 9700408), only the virulent strains produced aggregates and formed visible filaments attached to the glass tube. These results suggest, although for a limited number of strains, that aggregation in such a medium could be used as a diagnostic tool to discriminate virulent strains of S. enteritidis. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29645 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-296452020-03-04T00:45:59Z Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test Solano, C. (Cristina) Sesma, B. (Begoña) Alvarez, M. (Miguel) Humphrey, T.J. (T. J.) Thorns, C.J. (C. J.) Gamazo, C. (Carlos) Polyacrylamide gels Phage type-4 Expression Chickens Microbiology The objective of this study was the in vitro differentiation of isolates of Salmonella enteritidis whose virulences differed in a chick model. A total of 14 strains of S. enteritidis were isolated from either the environment, dairy products, or infected patients. The isolates could be divided into two groups on the basis of their virulence (50% lethal dose) in chickens infected intraperitoneally. When the strains were incubated in adherence test medium (Spanish patent 9700408), only the virulent strains produced aggregates and formed visible filaments attached to the glass tube. These results suggest, although for a limited number of strains, that aggregation in such a medium could be used as a diagnostic tool to discriminate virulent strains of S. enteritidis. 2013-09-02T10:22:42Z 2013-09-02T10:22:42Z 1998 info:eu-repo/semantics/article https://hdl.handle.net/10171/29645 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Polyacrylamide gels Phage type-4 Expression Chickens Microbiology Solano, C. (Cristina) Sesma, B. (Begoña) Alvarez, M. (Miguel) Humphrey, T.J. (T. J.) Thorns, C.J. (C. J.) Gamazo, C. (Carlos) Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test |
| title | Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test |
| title_full | Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test |
| title_fullStr | Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test |
| title_full_unstemmed | Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test |
| title_short | Discrimination of strains of Salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test |
| title_sort | discrimination of strains of salmonella enteritidis with differing levels of virulence by an in vitro glass adherence test |
| topic | Polyacrylamide gels Phage type-4 Expression Chickens Microbiology |
| url | https://hdl.handle.net/10171/29645 |
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