Towards a non-living vaccine for shigellosis
Shigellosis is an acute invasive enteric infection caused by Shigella spp. with an incidence of 165 millions episodes leading to 1.1 millions deaths. Considering bacteria easily spread from person to person and the emergence of multidrug- resistant Shigella strains, WHO gave high priority for the de...
| Main Authors: | , , |
|---|---|
| Format: | info:eu-repo/semantics/doctoralThesis |
| Language: | eng spa |
| Published: |
Servicio de Publicaciones de la Universidad de Navarra
2015
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/38071 |
| _version_ | 1793400456884518912 |
|---|---|
| author | Camacho, A.I. (A.I.) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) |
| author_facet | Camacho, A.I. (A.I.) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) |
| author_sort | Camacho, A.I. (A.I.) |
| collection | DSpace |
| description | Shigellosis is an acute invasive enteric infection caused by Shigella spp. with an incidence of 165 millions episodes leading to 1.1 millions deaths. Considering bacteria easily spread from person to person and the emergence of multidrug- resistant Shigella strains, WHO gave high priority for the development of a shigellosis vaccine in 1991.
Unfortunately, none of the proposals have an acceptable success. On this context, our proposal is based of the employment of an acellular vaccine for mucosal administration, containing relevant antigens from Shigella. Moreover, in order to increase its efficacy, it was propose the use of nanoparticles as adjuvants. Considering pathogenesis of Shigella, we select outer membrane vesicles (OMVs) as the subcellular extract. Since they are part of the outer membrane of bacteria, OMV extraction seems to be a effective strategy for isolation of the most relevant antigens of bacteria.
Nevertheless, safety of this kind of subcellular vaccines is usually hampered with a lower inherent immunogenicity. Consequently, in order to obtain an adequate immune response and obtain a delivery system that allow mucosal administration, the antigenic extract from Shigella would be encapsulated in Gantrez® AN, a copolymer of ether and maleic anhydride (PMV/MA) with bioadhesive properties. |
| format | info:eu-repo/semantics/doctoralThesis |
| id | oai:dadun.unav.edu:10171-38071 |
| institution | Universidad de Navarra |
| language | eng spa |
| publishDate | 2015 |
| publisher | Servicio de Publicaciones de la Universidad de Navarra |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-380712020-03-03T13:44:33Z Towards a non-living vaccine for shigellosis Camacho, A.I. (A.I.) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) Vacunas Microbiología Materias Investigacion::Farmacia Shigellosis is an acute invasive enteric infection caused by Shigella spp. with an incidence of 165 millions episodes leading to 1.1 millions deaths. Considering bacteria easily spread from person to person and the emergence of multidrug- resistant Shigella strains, WHO gave high priority for the development of a shigellosis vaccine in 1991. Unfortunately, none of the proposals have an acceptable success. On this context, our proposal is based of the employment of an acellular vaccine for mucosal administration, containing relevant antigens from Shigella. Moreover, in order to increase its efficacy, it was propose the use of nanoparticles as adjuvants. Considering pathogenesis of Shigella, we select outer membrane vesicles (OMVs) as the subcellular extract. Since they are part of the outer membrane of bacteria, OMV extraction seems to be a effective strategy for isolation of the most relevant antigens of bacteria. Nevertheless, safety of this kind of subcellular vaccines is usually hampered with a lower inherent immunogenicity. Consequently, in order to obtain an adequate immune response and obtain a delivery system that allow mucosal administration, the antigenic extract from Shigella would be encapsulated in Gantrez® AN, a copolymer of ether and maleic anhydride (PMV/MA) with bioadhesive properties. 2015-04-17T10:35:11Z 2015-04-17T10:35:11Z 2015 2012-11-28 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/10171/38071 eng spa info:eu-repo/semantics/openAccess application/pdf Servicio de Publicaciones de la Universidad de Navarra |
| spellingShingle | Vacunas Microbiología Materias Investigacion::Farmacia Camacho, A.I. (A.I.) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) Towards a non-living vaccine for shigellosis |
| title | Towards a non-living vaccine for shigellosis |
| title_full | Towards a non-living vaccine for shigellosis |
| title_fullStr | Towards a non-living vaccine for shigellosis |
| title_full_unstemmed | Towards a non-living vaccine for shigellosis |
| title_short | Towards a non-living vaccine for shigellosis |
| title_sort | towards a non-living vaccine for shigellosis |
| topic | Vacunas Microbiología Materias Investigacion::Farmacia |
| url | https://hdl.handle.net/10171/38071 |
| work_keys_str_mv | AT camachoaiai towardsanonlivingvaccineforshigellosis AT irachejmjuanmanuel towardsanonlivingvaccineforshigellosis AT gamazoccarlos towardsanonlivingvaccineforshigellosis |