Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved
α-lipoic acid (5-(1,2-dithiolan-3-yl)-pentanoic acid) is a natural occurring compound which possesses antioxidant properties. Moreover, anti-obesity properties in both rodents and humans have been attributed to α-lipoic acid being adipose tissue a target for this compound. In this context, the prese...
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| Format: | info:eu-repo/semantics/doctoralThesis |
| Language: | eng |
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2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/39790 |
| _version_ | 1793400479423660032 |
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| author | Fernandez-Galilea, M. (Marta) Perez-Matute, P. (Patricia) Moreno-Aliaga, M. J. (María Jesús) |
| author_facet | Fernandez-Galilea, M. (Marta) Perez-Matute, P. (Patricia) Moreno-Aliaga, M. J. (María Jesús) |
| author_sort | Fernandez-Galilea, M. (Marta) |
| collection | DSpace |
| description | α-lipoic acid (5-(1,2-dithiolan-3-yl)-pentanoic acid) is a natural occurring compound which possesses antioxidant properties. Moreover, anti-obesity properties in both rodents and humans have been attributed to α-lipoic acid being adipose tissue a target for this compound. In this context, the present study has demonstrated the ability of α-LA to modulate lipid metabolism in both murine and human white adipocytes. Thus, α-LA: 1) Inhibits fatty acids uptake (through CD36 downregulation); 2) Reduces fatty acids esterification into triglycerides (through DGAT1 reduction); 3) Decreases de novo lipogenesis (induced by inhibition of FAS, SCD1 and ACC mediated by AMPK activation); 4) Stimulates lipolysis (mainly mediated by the phosphorylation of HSL through cAMP-mediated activation of PKA, probably through the inhibition of AdPLA and PGE2; 5) Increases fatty acid oxidation machinery (CPT1 and ACOX) in white adipocytes; 6) Promotes mitochondrial biogenesis by the activation of PGC-1α mediated by SIRT1 and AMPK activation; and 7) Induces a brown-like remodelling in white subcutaneous adipocytes from overweight/obese subjects.
Taking together all of these facts suggest that α-LA remodels adipocyte metabolism towards oxidation rather than storage, and this could contribute to the anti-obesity properties of α-LA. |
| format | info:eu-repo/semantics/doctoralThesis |
| id | oai:dadun.unav.edu:10171-39790 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2016 |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-397902020-03-03T13:13:17Z Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved Fernandez-Galilea, M. (Marta) Perez-Matute, P. (Patricia) Moreno-Aliaga, M. J. (María Jesús) Adipocytes Lipid metabolism Obesity α-Lipoic acid Nutrientes Biología humana Metabolismo energético Biología celular α-lipoic acid (5-(1,2-dithiolan-3-yl)-pentanoic acid) is a natural occurring compound which possesses antioxidant properties. Moreover, anti-obesity properties in both rodents and humans have been attributed to α-lipoic acid being adipose tissue a target for this compound. In this context, the present study has demonstrated the ability of α-LA to modulate lipid metabolism in both murine and human white adipocytes. Thus, α-LA: 1) Inhibits fatty acids uptake (through CD36 downregulation); 2) Reduces fatty acids esterification into triglycerides (through DGAT1 reduction); 3) Decreases de novo lipogenesis (induced by inhibition of FAS, SCD1 and ACC mediated by AMPK activation); 4) Stimulates lipolysis (mainly mediated by the phosphorylation of HSL through cAMP-mediated activation of PKA, probably through the inhibition of AdPLA and PGE2; 5) Increases fatty acid oxidation machinery (CPT1 and ACOX) in white adipocytes; 6) Promotes mitochondrial biogenesis by the activation of PGC-1α mediated by SIRT1 and AMPK activation; and 7) Induces a brown-like remodelling in white subcutaneous adipocytes from overweight/obese subjects. Taking together all of these facts suggest that α-LA remodels adipocyte metabolism towards oxidation rather than storage, and this could contribute to the anti-obesity properties of α-LA. 2016-01-25T12:24:15Z 2016-01-25T12:24:15Z 2016 2013-12-18 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/10171/39790 eng info:eu-repo/semantics/openAccess application/pdf |
| spellingShingle | Adipocytes Lipid metabolism Obesity α-Lipoic acid Nutrientes Biología humana Metabolismo energético Biología celular Fernandez-Galilea, M. (Marta) Perez-Matute, P. (Patricia) Moreno-Aliaga, M. J. (María Jesús) Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved |
| title | Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved |
| title_full | Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved |
| title_fullStr | Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved |
| title_full_unstemmed | Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved |
| title_short | Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved |
| title_sort | effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved |
| topic | Adipocytes Lipid metabolism Obesity α-Lipoic acid Nutrientes Biología humana Metabolismo energético Biología celular |
| url | https://hdl.handle.net/10171/39790 |
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