Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective
The genome of a tumor cell presents thousands of genomic alterations including base-substitutions and somatic copy number alterations (SCNAs). SCNAs comprise amplifications and deletions of big chromosomal regions usually containing hundreds of genes. Some of these regions harbor well-studied cancer...
| Main Authors: | , |
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| Format: | info:eu-repo/semantics/doctoralThesis |
| Language: | eng |
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2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/45008 |
| _version_ | 1793400460255690752 |
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| author | Athie-Cuervo, A. (Alejandro) Huarte-Martínez, M. (Maite) |
| author_facet | Athie-Cuervo, A. (Alejandro) Huarte-Martínez, M. (Maite) |
| author_sort | Athie-Cuervo, A. (Alejandro) |
| collection | DSpace |
| description | The genome of a tumor cell presents thousands of genomic alterations including base-substitutions and somatic copy number alterations (SCNAs). SCNAs comprise amplifications and deletions of big chromosomal regions usually containing hundreds of genes. Some of these regions harbor well-studied cancer drivers; however many others do not contain a known driver. The analysis of SCNA focusing on the non-coding genome helped us pinpoint a list of copy number altered long noncoding RNAs (lncRNAs). In order to validate our findings we experimentally characterized functionally and mechanistically a lncRNA amplified in lung cancer which we named LUAD-amp-1. LUAD-amp-1 acts as an oncogenic lncRNA, and its expression is induced by the transcription factor NF-B upon TNF treatment. Moreover, LUAD-amp-1 is implicated in the inhibition of a set of NF-B regulated genes including TNF itself. LUAD-amp-1 molecular mechanism relies on its association with SART3, altering its localization and modulating the nuclear translocation of its associated protein USP4. |
| format | info:eu-repo/semantics/doctoralThesis |
| id | oai:dadun.unav.edu:10171-45008 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2017 |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-450082024-02-08T10:52:19Z Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective Athie-Cuervo, A. (Alejandro) Huarte-Martínez, M. (Maite) Materias Investigacion::Ciencias de la Salud::Oncología The genome of a tumor cell presents thousands of genomic alterations including base-substitutions and somatic copy number alterations (SCNAs). SCNAs comprise amplifications and deletions of big chromosomal regions usually containing hundreds of genes. Some of these regions harbor well-studied cancer drivers; however many others do not contain a known driver. The analysis of SCNA focusing on the non-coding genome helped us pinpoint a list of copy number altered long noncoding RNAs (lncRNAs). In order to validate our findings we experimentally characterized functionally and mechanistically a lncRNA amplified in lung cancer which we named LUAD-amp-1. LUAD-amp-1 acts as an oncogenic lncRNA, and its expression is induced by the transcription factor NF-B upon TNF treatment. Moreover, LUAD-amp-1 is implicated in the inhibition of a set of NF-B regulated genes including TNF itself. LUAD-amp-1 molecular mechanism relies on its association with SART3, altering its localization and modulating the nuclear translocation of its associated protein USP4. 2017-12-07T07:59:56Z 2017-12-07T07:59:56Z 2017-12-05 2017-09-28 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/10171/45008 eng info:eu-repo/semantics/openAccess application/pdf |
| spellingShingle | Materias Investigacion::Ciencias de la Salud::Oncología Athie-Cuervo, A. (Alejandro) Huarte-Martínez, M. (Maite) Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective |
| title | Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective |
| title_full | Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective |
| title_fullStr | Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective |
| title_full_unstemmed | Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective |
| title_short | Somatic Copy-Number Alterations across Human Cancers from LncRNA Perspective |
| title_sort | somatic copy-number alterations across human cancers from lncrna perspective |
| topic | Materias Investigacion::Ciencias de la Salud::Oncología |
| url | https://hdl.handle.net/10171/45008 |
| work_keys_str_mv | AT athiecuervoaalejandro somaticcopynumberalterationsacrosshumancancersfromlncrnaperspective AT huartemartinezmmaite somaticcopynumberalterationsacrosshumancancersfromlncrnaperspective |