3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices
Microfluidic devices filled with hydrogels that mimic the extracellular matrix are powerful and versatile research tools to recapitulate the 3D tumor microenvironment and study cancer in vitro. In this work, H1299 NSCLC cell migration phenotype and dynamics are characterized in hydrogels that mimic...
| Main Authors: | , |
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| Format: | info:eu-repo/semantics/doctoralThesis |
| Language: | eng |
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Servicio de Publicaciones. Universidad de Navarra
2018
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| Online Access: | https://hdl.handle.net/10171/55768 |
| _version_ | 1793400612379951104 |
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| author | Anguiano-Salcedo, M.(María) Ortiz-de-Solorzano, C. (Carlos) |
| author_facet | Anguiano-Salcedo, M.(María) Ortiz-de-Solorzano, C. (Carlos) |
| author_sort | Anguiano-Salcedo, M.(María) |
| collection | DSpace |
| description | Microfluidic devices filled with hydrogels that mimic the extracellular matrix are powerful and versatile research tools to recapitulate the 3D tumor microenvironment and study cancer in vitro. In this work, H1299 NSCLC cell migration phenotype and dynamics are characterized in hydrogels that mimic different tumor microenvironments, from pure collagen matrices similar to connective tissue to mixed collagen-Matrigel matrices that approximate a disorganized basement membrane at the front of cancer invasion. The role of the microenvironment composition and mechanical properties, cell adhesion, MMP proteolytic activity and integrin surface expression in the migration behavior of H1299 lung cancer cells within these 3D matrices are explored and explained in light of what it is known about cell migration in 3D environments. Finally, the extracellular matrix remodeling caused by H1299 cells in the different microenvironments is quantified by measuring the ability of H1299 cells to degrade, align, and compact their surrounding matrix. |
| format | info:eu-repo/semantics/doctoralThesis |
| id | oai:dadun.unav.edu:10171-55768 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2018 |
| publisher | Servicio de Publicaciones. Universidad de Navarra |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-557682022-02-08T08:57:19Z 3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices Anguiano-Salcedo, M.(María) Ortiz-de-Solorzano, C. (Carlos) Microfluidic devices filled with hydrogels that mimic the extracellular matrix are powerful and versatile research tools to recapitulate the 3D tumor microenvironment and study cancer in vitro. In this work, H1299 NSCLC cell migration phenotype and dynamics are characterized in hydrogels that mimic different tumor microenvironments, from pure collagen matrices similar to connective tissue to mixed collagen-Matrigel matrices that approximate a disorganized basement membrane at the front of cancer invasion. The role of the microenvironment composition and mechanical properties, cell adhesion, MMP proteolytic activity and integrin surface expression in the migration behavior of H1299 lung cancer cells within these 3D matrices are explored and explained in light of what it is known about cell migration in 3D environments. Finally, the extracellular matrix remodeling caused by H1299 cells in the different microenvironments is quantified by measuring the ability of H1299 cells to degrade, align, and compact their surrounding matrix. 2018-11-12T11:13:39Z 2018-11-12T11:13:39Z 2018-10 2018-10-08 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/10171/55768 eng info:eu-repo/semantics/openAccess application/pdf Servicio de Publicaciones. Universidad de Navarra |
| spellingShingle | Anguiano-Salcedo, M.(María) Ortiz-de-Solorzano, C. (Carlos) 3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices |
| title | 3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices |
| title_full | 3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices |
| title_fullStr | 3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices |
| title_full_unstemmed | 3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices |
| title_short | 3D lung cancer cell migration and extracellular matrix remodeling within microfluidic devices |
| title_sort | 3d lung cancer cell migration and extracellular matrix remodeling within microfluidic devices |
| url | https://hdl.handle.net/10171/55768 |
| work_keys_str_mv | AT anguianosalcedommaria 3dlungcancercellmigrationandextracellularmatrixremodelingwithinmicrofluidicdevices AT ortizdesolorzanoccarlos 3dlungcancercellmigrationandextracellularmatrixremodelingwithinmicrofluidicdevices |