The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus
Brucellosis is a worldwide extended zoonosis caused by Brucella spp. These gram-negative bacteria are not readily detected by innate immunity, a virulence-related property largely linked to their surface lipopolysaccharide (LPS). The role of the LPS lipid A and O-polysaccharide in virulence is well...
| Main Authors: | , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10171/56052 |
| _version_ | 1793400084192296960 |
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| author | Gil-Ramirez, Y. (Yolanda) Raquel Palacios-Chaves, L. (Leyre) Zuñiga-Ripa, A. (Amaia) Grillo, M.J. (María Jesús) Arce-Gorvel, V. (Vilma) Hanniffy, S. (Sean) Moriyon, I. (Ignacio) Iriarte, M. (Maite) |
| author_facet | Gil-Ramirez, Y. (Yolanda) Raquel Palacios-Chaves, L. (Leyre) Zuñiga-Ripa, A. (Amaia) Grillo, M.J. (María Jesús) Arce-Gorvel, V. (Vilma) Hanniffy, S. (Sean) Moriyon, I. (Ignacio) Iriarte, M. (Maite) |
| author_sort | Gil-Ramirez, Y. (Yolanda) |
| collection | DSpace |
| description | Brucellosis is a worldwide extended zoonosis caused by Brucella spp. These gram-negative bacteria are not readily detected by innate immunity, a virulence-related property largely linked to their surface lipopolysaccharide (LPS). The role of the LPS lipid A and O-polysaccharide in virulence is well known. Moreover, mutation of the glycosyltransferase gene wadC of Brucella abortus, although not affecting O-polysaccharide assembly onto the lipid-A core section causes a core oligosaccharide defect that increases recognition by innate immunity. Here, we report on a second gene (wadB) encoding a LPS core glycosyltransferase not involved in the assembly of the O-polysaccharide-linked core section. As compared to wild-type B. abortus, a wadB mutant was sensitive to bactericidal peptides and non-immune serum, and was attenuated in mice and dendritic cells. These observations show that as WadC, WadB is also involved in the assembly of a branch of Brucella LPS core and support the concept that this LPS section is a virulence-related structure. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-56052 |
| institution | Universidad de Navarra |
| language | English |
| publishDate | 2019 |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-560522022-11-28T11:34:00Z The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus Gil-Ramirez, Y. (Yolanda) Raquel Palacios-Chaves, L. (Leyre) Zuñiga-Ripa, A. (Amaia) Grillo, M.J. (María Jesús) Arce-Gorvel, V. (Vilma) Hanniffy, S. (Sean) Moriyon, I. (Ignacio) Iriarte, M. (Maite) Brucella Glycosyltransferase Lipopolysaccharide Virulence WadB WadC Brucellosis is a worldwide extended zoonosis caused by Brucella spp. These gram-negative bacteria are not readily detected by innate immunity, a virulence-related property largely linked to their surface lipopolysaccharide (LPS). The role of the LPS lipid A and O-polysaccharide in virulence is well known. Moreover, mutation of the glycosyltransferase gene wadC of Brucella abortus, although not affecting O-polysaccharide assembly onto the lipid-A core section causes a core oligosaccharide defect that increases recognition by innate immunity. Here, we report on a second gene (wadB) encoding a LPS core glycosyltransferase not involved in the assembly of the O-polysaccharide-linked core section. As compared to wild-type B. abortus, a wadB mutant was sensitive to bactericidal peptides and non-immune serum, and was attenuated in mice and dendritic cells. These observations show that as WadC, WadB is also involved in the assembly of a branch of Brucella LPS core and support the concept that this LPS section is a virulence-related structure. 2019-01-04T08:29:24Z 2019-01-04T08:29:24Z 2014 info:eu-repo/semantics/article https://hdl.handle.net/10171/56052 en info:eu-repo/semantics/openAccess application/pdf |
| spellingShingle | Brucella Glycosyltransferase Lipopolysaccharide Virulence WadB WadC Gil-Ramirez, Y. (Yolanda) Raquel Palacios-Chaves, L. (Leyre) Zuñiga-Ripa, A. (Amaia) Grillo, M.J. (María Jesús) Arce-Gorvel, V. (Vilma) Hanniffy, S. (Sean) Moriyon, I. (Ignacio) Iriarte, M. (Maite) The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus |
| title | The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus |
| title_full | The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus |
| title_fullStr | The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus |
| title_full_unstemmed | The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus |
| title_short | The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus |
| title_sort | identification of wadb, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of brucella abortus |
| topic | Brucella Glycosyltransferase Lipopolysaccharide Virulence WadB WadC |
| url | https://hdl.handle.net/10171/56052 |
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