Comet assay modifications for its application in food safety
Information on genotoxicity is of a key importance for the toxicological characterisation of different compounds. In this vein, and due to its various advantages, the comet assay is currently included in the genotoxicity testing strategy used in the food safety area. However, improvement points of p...
| Main Authors: | , , , |
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| Format: | info:eu-repo/semantics/doctoralThesis |
| Language: | eng |
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2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/56328 |
| _version_ | 1793400594427281408 |
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| author | Enciso-Gadea, J. M. (José Manuel) Lopez-de-Cerain, A. (Adela) Azqueta, A. (Amaya) Vettorazzi, A. (Ariane) |
| author_facet | Enciso-Gadea, J. M. (José Manuel) Lopez-de-Cerain, A. (Adela) Azqueta, A. (Amaya) Vettorazzi, A. (Ariane) |
| author_sort | Enciso-Gadea, J. M. (José Manuel) |
| collection | DSpace |
| description | Information on genotoxicity is of a key importance for the toxicological characterisation of different compounds. In this vein, and due to its various advantages, the comet assay is currently included in the genotoxicity testing strategy used in the food safety area. However, improvement points of particular interest have been identified. Thereby, the main objective of the present work was to evaluate some critical points of the comet assay, such as the time of lysis, in vitro, and the methodology used in the freezing/thawing procedures of tissue samples, their stability and the application of the Fpg-modified assay, in vivo. In addition, the in vivo comet assay was applied to frozen kidney samples obtained in a previous repeated-dose toxicity study of the food contaminant ochratoxin A. Finally, the knowledge derived from these objectives resulted in the development of standard operating procedures for both the in vitro and in vivo comet assays, which could be applied in good laboratory practice studies. |
| format | info:eu-repo/semantics/doctoralThesis |
| id | oai:dadun.unav.edu:10171-56328 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2019 |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-563282023-04-13T10:50:20Z Comet assay modifications for its application in food safety Enciso-Gadea, J. M. (José Manuel) Lopez-de-Cerain, A. (Adela) Azqueta, A. (Amaya) Vettorazzi, A. (Ariane) comet assay genotoxicity food safety ochratoxin A Materias Investigacion::Ciencias de la Salud::Nutrición y dietética Information on genotoxicity is of a key importance for the toxicological characterisation of different compounds. In this vein, and due to its various advantages, the comet assay is currently included in the genotoxicity testing strategy used in the food safety area. However, improvement points of particular interest have been identified. Thereby, the main objective of the present work was to evaluate some critical points of the comet assay, such as the time of lysis, in vitro, and the methodology used in the freezing/thawing procedures of tissue samples, their stability and the application of the Fpg-modified assay, in vivo. In addition, the in vivo comet assay was applied to frozen kidney samples obtained in a previous repeated-dose toxicity study of the food contaminant ochratoxin A. Finally, the knowledge derived from these objectives resulted in the development of standard operating procedures for both the in vitro and in vivo comet assays, which could be applied in good laboratory practice studies. 2019-01-16T15:28:37Z 2019-01-16T15:28:37Z 2019-01-16 2018-03-12 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/10171/56328 eng info:eu-repo/semantics/openAccess application/pdf |
| spellingShingle | comet assay genotoxicity food safety ochratoxin A Materias Investigacion::Ciencias de la Salud::Nutrición y dietética Enciso-Gadea, J. M. (José Manuel) Lopez-de-Cerain, A. (Adela) Azqueta, A. (Amaya) Vettorazzi, A. (Ariane) Comet assay modifications for its application in food safety |
| title | Comet assay modifications for its application in food safety |
| title_full | Comet assay modifications for its application in food safety |
| title_fullStr | Comet assay modifications for its application in food safety |
| title_full_unstemmed | Comet assay modifications for its application in food safety |
| title_short | Comet assay modifications for its application in food safety |
| title_sort | comet assay modifications for its application in food safety |
| topic | comet assay genotoxicity food safety ochratoxin A Materias Investigacion::Ciencias de la Salud::Nutrición y dietética |
| url | https://hdl.handle.net/10171/56328 |
| work_keys_str_mv | AT encisogadeajmjosemanuel cometassaymodificationsforitsapplicationinfoodsafety AT lopezdecerainaadela cometassaymodificationsforitsapplicationinfoodsafety AT azquetaaamaya cometassaymodificationsforitsapplicationinfoodsafety AT vettorazziaariane cometassaymodificationsforitsapplicationinfoodsafety |