Dissolving microneedles for intradermal vaccination against shigellosis
Intradermal (ID) immunization is of increasing interest due to the easy accessibility and excellent immunogenic properties of the skin. Among ID immunization methods, dissolving microneedles (MNs) have appeared as an alternative to traditional hypodermic immunization, offering many advantages, such...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10171/58473 |
| _version_ | 1793400393745563648 |
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| author | Gamazo, C. (Carlos) Donnelly, R. (Ryan) Irache, J.M. (Juan Manuel) Peñuelas-Sanchez, I. (Ivan) Quincoces, G. (Gemma) Erhard, Á. (Álvaro) Larrañeta, E. (Eneko) Pastor, Y. (Yadira) |
| author_facet | Gamazo, C. (Carlos) Donnelly, R. (Ryan) Irache, J.M. (Juan Manuel) Peñuelas-Sanchez, I. (Ivan) Quincoces, G. (Gemma) Erhard, Á. (Álvaro) Larrañeta, E. (Eneko) Pastor, Y. (Yadira) |
| author_sort | Gamazo, C. (Carlos) |
| collection | DSpace |
| description | Intradermal (ID) immunization is of increasing interest due to the easy accessibility and excellent immunogenic properties of the skin. Among ID immunization methods, dissolving microneedles (MNs) have appeared as an alternative to traditional hypodermic immunization, offering many advantages, such as being an easily administered method, with no need for health personnel, painless, and avoiding the use of needles and sharp wastage. In this study, an affordable and easy-to-produce MNs method was developed based on aqueous blends of 30% w/w poly (methyl vinyl ether-co-maleic anhydride). As an antigen model, a subunit vaccine candidate based on outer membrane vesicles from Shigella flexneri was used. Both unloaded and antigen-loaded MNs were synthetized and characterized. The MNs were successfully validated in an in vitro Parafilm M skin model and in a pig skin ex vivo model. Biodistribution studies were performed in BALB/c mice using 99mTcO4- radiolabeled samples. Results indicated that the vesicle vaccine was successfully released from the MNs and targeted gastrointestinal tract after 6 h post-administration. In vivo immunization and protection studies were performed in BALB/c mice. Mice were intradermally immunized through ear skin with one single dose of 200 g antigenic complex, eliciting the production of specific systemic IgG and mucosal IgA. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-58473 |
| institution | Universidad de Navarra |
| language | English |
| publishDate | 2019 |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-584732022-07-20T06:32:20Z Dissolving microneedles for intradermal vaccination against shigellosis Gamazo, C. (Carlos) Donnelly, R. (Ryan) Irache, J.M. (Juan Manuel) Peñuelas-Sanchez, I. (Ivan) Quincoces, G. (Gemma) Erhard, Á. (Álvaro) Larrañeta, E. (Eneko) Pastor, Y. (Yadira) Dissolving microneedles Intradermal Shigella Vaccine Outer membrane vesicles Intradermal (ID) immunization is of increasing interest due to the easy accessibility and excellent immunogenic properties of the skin. Among ID immunization methods, dissolving microneedles (MNs) have appeared as an alternative to traditional hypodermic immunization, offering many advantages, such as being an easily administered method, with no need for health personnel, painless, and avoiding the use of needles and sharp wastage. In this study, an affordable and easy-to-produce MNs method was developed based on aqueous blends of 30% w/w poly (methyl vinyl ether-co-maleic anhydride). As an antigen model, a subunit vaccine candidate based on outer membrane vesicles from Shigella flexneri was used. Both unloaded and antigen-loaded MNs were synthetized and characterized. The MNs were successfully validated in an in vitro Parafilm M skin model and in a pig skin ex vivo model. Biodistribution studies were performed in BALB/c mice using 99mTcO4- radiolabeled samples. Results indicated that the vesicle vaccine was successfully released from the MNs and targeted gastrointestinal tract after 6 h post-administration. In vivo immunization and protection studies were performed in BALB/c mice. Mice were intradermally immunized through ear skin with one single dose of 200 g antigenic complex, eliciting the production of specific systemic IgG and mucosal IgA. 2019-11-18T11:43:23Z 2019-11-18T11:43:23Z 2019 info:eu-repo/semantics/article https://hdl.handle.net/10171/58473 en info:eu-repo/grantAgreement/WT/Physiology in Health and Disease/094085 info:eu-repo/semantics/openAccess application/pdf |
| spellingShingle | Dissolving microneedles Intradermal Shigella Vaccine Outer membrane vesicles Gamazo, C. (Carlos) Donnelly, R. (Ryan) Irache, J.M. (Juan Manuel) Peñuelas-Sanchez, I. (Ivan) Quincoces, G. (Gemma) Erhard, Á. (Álvaro) Larrañeta, E. (Eneko) Pastor, Y. (Yadira) Dissolving microneedles for intradermal vaccination against shigellosis |
| title | Dissolving microneedles for intradermal vaccination against shigellosis |
| title_full | Dissolving microneedles for intradermal vaccination against shigellosis |
| title_fullStr | Dissolving microneedles for intradermal vaccination against shigellosis |
| title_full_unstemmed | Dissolving microneedles for intradermal vaccination against shigellosis |
| title_short | Dissolving microneedles for intradermal vaccination against shigellosis |
| title_sort | dissolving microneedles for intradermal vaccination against shigellosis |
| topic | Dissolving microneedles Intradermal Shigella Vaccine Outer membrane vesicles |
| url | https://hdl.handle.net/10171/58473 |
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