Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain
Mobile genetic elements play an important role in the dissemination of antibiotic resistant bacteria among human and environmental sources. Therefore, the aim of this study was to determine the occurrence and patterns of integrons and insertion sequences of extended-spectrum β-lactamase (ESBL)-pr...
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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MDPI AG
2022
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| Online Access: | https://hdl.handle.net/10171/64391 |
| _version_ | 1793400044171296768 |
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| author | Pérez-Etayo, L. (Lara) Berzosa-Suñer, M. (Melibea) González, D. (David) Vitas, A.I. (Ana Isabel) |
| author_facet | Pérez-Etayo, L. (Lara) Berzosa-Suñer, M. (Melibea) González, D. (David) Vitas, A.I. (Ana Isabel) |
| author_sort | Pérez-Etayo, L. (Lara) |
| collection | DSpace |
| description | Mobile genetic elements play an important role in the dissemination of antibiotic resistant
bacteria among human and environmental sources. Therefore, the aim of this study was to determine
the occurrence and patterns of integrons and insertion sequences of extended-spectrum β-lactamase
(ESBL)-producing Escherichia coli isolated from different sources in Navarra, northern Spain. A total
of 150 isolates coming from food products, farms and feeds, aquatic environments, and humans
(healthy people and hospital inpatients), were analyzed. PCRs were applied for the study of class
1, 2, and 3 integrons (intI1, intI2, and intI3), as well as for the determination of insertion sequences
(IS26, ISEcp1, ISCR1, and IS903). Results show the wide presence and dissemination of intI1 (92%),
while intI3 was not detected. It is remarkable, the prevalence of intI2 among food isolates, as well
as the co-existence of class 1 and class 2 (8% of isolates). The majority of isolates have two or
three IS elements, with the most common being IS26 (99.4%). The genetic pattern IS26–ISEcp1
(related with the pathogen clone ST131) was present in the 22% of isolates (including human isolates).
In addition, the combination ISEcp1–IS26–IS903–ISCR1 was detected in 11 isolates being, to our
knowledge, the first study that describes this genetic complex. Due to the wide variability observed,
no relationship was determined among these mobile genetic elements and β-lactam resistance.
More investigations regarding the genetic composition of these elements are needed to understand
the role of multiple types of integrons and insertion sequences on the dissemination of antimicrobial
resistance genes among different environments. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-64391 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2022 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-643912022-10-07T01:07:01Z Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain Pérez-Etayo, L. (Lara) Berzosa-Suñer, M. (Melibea) González, D. (David) Vitas, A.I. (Ana Isabel) ESBL-producing E. coli β-lactamase genes Antimicrobial resistance Integrons Insertion sequences Mobile genetic elements play an important role in the dissemination of antibiotic resistant bacteria among human and environmental sources. Therefore, the aim of this study was to determine the occurrence and patterns of integrons and insertion sequences of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from different sources in Navarra, northern Spain. A total of 150 isolates coming from food products, farms and feeds, aquatic environments, and humans (healthy people and hospital inpatients), were analyzed. PCRs were applied for the study of class 1, 2, and 3 integrons (intI1, intI2, and intI3), as well as for the determination of insertion sequences (IS26, ISEcp1, ISCR1, and IS903). Results show the wide presence and dissemination of intI1 (92%), while intI3 was not detected. It is remarkable, the prevalence of intI2 among food isolates, as well as the co-existence of class 1 and class 2 (8% of isolates). The majority of isolates have two or three IS elements, with the most common being IS26 (99.4%). The genetic pattern IS26–ISEcp1 (related with the pathogen clone ST131) was present in the 22% of isolates (including human isolates). In addition, the combination ISEcp1–IS26–IS903–ISCR1 was detected in 11 isolates being, to our knowledge, the first study that describes this genetic complex. Due to the wide variability observed, no relationship was determined among these mobile genetic elements and β-lactam resistance. More investigations regarding the genetic composition of these elements are needed to understand the role of multiple types of integrons and insertion sequences on the dissemination of antimicrobial resistance genes among different environments. 2022-10-06T08:15:09Z 2022-10-06T08:15:09Z 2018 info:eu-repo/semantics/article https://hdl.handle.net/10171/64391 eng info:eu-repo/semantics/openAccess application/pdf MDPI AG |
| spellingShingle | ESBL-producing E. coli β-lactamase genes Antimicrobial resistance Integrons Insertion sequences Pérez-Etayo, L. (Lara) Berzosa-Suñer, M. (Melibea) González, D. (David) Vitas, A.I. (Ana Isabel) Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain |
| title | Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain |
| title_full | Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain |
| title_fullStr | Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain |
| title_full_unstemmed | Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain |
| title_short | Prevalence of integrons and insertion sequences in ESBL-producing E-coli isolated from different sources in Navarra, Spain |
| title_sort | prevalence of integrons and insertion sequences in esbl-producing e-coli isolated from different sources in navarra, spain |
| topic | ESBL-producing E. coli β-lactamase genes Antimicrobial resistance Integrons Insertion sequences |
| url | https://hdl.handle.net/10171/64391 |
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