Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect
The current work describes the capability of casein-chitosan microparticles to encapsulate Lactobacillus plantarum (CECT 220 and WCFS1 strains) and evaluates their ability to target the distal areas of the gut and to stimulate the immune system. Microparticles were prepared by complex coacervation...
| Main Authors: | , , , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Elsevier
2023
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| Online Access: | https://hdl.handle.net/10171/66144 |
| _version_ | 1793400183901388800 |
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| author | Peñalva, R. (Rebeca) López, A. (Andrés) Gamazo, C. (Carlos) Gonzalez-Navarro, C.J. (Carlos Javier) González-Ferrero, C. (Carolina) Virto-Resano, R. (Raquel) Brotons-Canto, A. (Ana) Vitas, A.I. (Ana Isabel) Collantes, M. (María) Peñuelas-Sanchez, I. (Ivan) Irache, J.M. (Juan Manuel) |
| author_facet | Peñalva, R. (Rebeca) López, A. (Andrés) Gamazo, C. (Carlos) Gonzalez-Navarro, C.J. (Carlos Javier) González-Ferrero, C. (Carolina) Virto-Resano, R. (Raquel) Brotons-Canto, A. (Ana) Vitas, A.I. (Ana Isabel) Collantes, M. (María) Peñuelas-Sanchez, I. (Ivan) Irache, J.M. (Juan Manuel) |
| author_sort | Peñalva, R. (Rebeca) |
| collection | DSpace |
| description | The current work describes the capability of casein-chitosan microparticles to encapsulate Lactobacillus plantarum
(CECT 220 and WCFS1 strains) and evaluates their ability to target the distal areas of the gut and to stimulate the
immune system. Microparticles were prepared by complex coacervation, between sodium caseinate and chitosan
in an aqueous suspension of the bacteria, and dried by spray-drying. In order to increase the survival rate of the
loaded bacteria, microparticles were cross-linked with one of the following cross-linkers: tripolyphosphate,
calcium salts or vanillin.
Overall, microparticles displayed a mean size of about 7.5 μm with a bacteria loading of about 11 Log CFU/g,
when cross-linked with vanillin (MP-LP-V). For conventional microparticles, the payload was 10.12 Log CFU/g.
The storage stability study at 25 ◦C/60% RH, MP-LP-V offered the highest degree of protection without signif-
icant modification of the payload in 260 days. Compared with control (aqueous suspension of bacteria), MP-LP-V
also displayed a significantly higher degree of protection against probiotic inactivation in simulated gastric and
intestinal fluids. In vivo results evidenced that microparticles, orally administered to rats, were able to reach the
distal ileum and colon in about 4 h post-administration. Additionally, the effect of the daily administration of
107 CFU/mouse of MP-LP-V, for 3 weeks, induced an immunomodulatory effect characterized by an important
enhancement of Th1 and Th17 responses. In conclusion, these microparticles seem to be a promising strategy for
increasing survival and efficacy of probiotics, allowing the formulation of cost-effective and more stable and
effective probiotic-based nutraceuticals. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-66144 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-661442024-01-29T13:15:33Z Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect Peñalva, R. (Rebeca) López, A. (Andrés) Gamazo, C. (Carlos) Gonzalez-Navarro, C.J. (Carlos Javier) González-Ferrero, C. (Carolina) Virto-Resano, R. (Raquel) Brotons-Canto, A. (Ana) Vitas, A.I. (Ana Isabel) Collantes, M. (María) Peñuelas-Sanchez, I. (Ivan) Irache, J.M. (Juan Manuel) Microparticles Casein Probiotics Lactobacillus plantarum Biodistribution Immunomodulation The current work describes the capability of casein-chitosan microparticles to encapsulate Lactobacillus plantarum (CECT 220 and WCFS1 strains) and evaluates their ability to target the distal areas of the gut and to stimulate the immune system. Microparticles were prepared by complex coacervation, between sodium caseinate and chitosan in an aqueous suspension of the bacteria, and dried by spray-drying. In order to increase the survival rate of the loaded bacteria, microparticles were cross-linked with one of the following cross-linkers: tripolyphosphate, calcium salts or vanillin. Overall, microparticles displayed a mean size of about 7.5 μm with a bacteria loading of about 11 Log CFU/g, when cross-linked with vanillin (MP-LP-V). For conventional microparticles, the payload was 10.12 Log CFU/g. The storage stability study at 25 ◦C/60% RH, MP-LP-V offered the highest degree of protection without signif- icant modification of the payload in 260 days. Compared with control (aqueous suspension of bacteria), MP-LP-V also displayed a significantly higher degree of protection against probiotic inactivation in simulated gastric and intestinal fluids. In vivo results evidenced that microparticles, orally administered to rats, were able to reach the distal ileum and colon in about 4 h post-administration. Additionally, the effect of the daily administration of 107 CFU/mouse of MP-LP-V, for 3 weeks, induced an immunomodulatory effect characterized by an important enhancement of Th1 and Th17 responses. In conclusion, these microparticles seem to be a promising strategy for increasing survival and efficacy of probiotics, allowing the formulation of cost-effective and more stable and effective probiotic-based nutraceuticals. 2023-05-03T08:02:38Z 2023-05-03T08:02:38Z 2023 info:eu-repo/semantics/article https://hdl.handle.net/10171/66144 eng info:eu-repo/semantics/openAccess application/pdf Elsevier |
| spellingShingle | Microparticles Casein Probiotics Lactobacillus plantarum Biodistribution Immunomodulation Peñalva, R. (Rebeca) López, A. (Andrés) Gamazo, C. (Carlos) Gonzalez-Navarro, C.J. (Carlos Javier) González-Ferrero, C. (Carolina) Virto-Resano, R. (Raquel) Brotons-Canto, A. (Ana) Vitas, A.I. (Ana Isabel) Collantes, M. (María) Peñuelas-Sanchez, I. (Ivan) Irache, J.M. (Juan Manuel) Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect |
| title | Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect |
| title_full | Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect |
| title_fullStr | Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect |
| title_full_unstemmed | Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect |
| title_short | Encapsulation of Lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect |
| title_sort | encapsulation of lactobacillus plantarum in casein-chitosan microparticles facilitates the arrival to the colon and develops an immunomodulatory effect |
| topic | Microparticles Casein Probiotics Lactobacillus plantarum Biodistribution Immunomodulation |
| url | https://hdl.handle.net/10171/66144 |
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