Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles
Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of illness and death in neonatal and recently weaned pigs. The immune protection of the piglets derives from maternal colostrum, since this species does not receive maternal antibodies through the placenta. In the present study, ou...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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MDPI AG
2023
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| Online Access: | https://hdl.handle.net/10171/67202 |
| _version_ | 1793400403451183104 |
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| author | Matías, J. (Jose) Brotons-Canto, A. (Ana) Cenoz, S. (Santiago) Pérez, I. (Isidoro) Abdulkarim, M. (Muthanna) Gumbleton, M. (Mark) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) |
| author_facet | Matías, J. (Jose) Brotons-Canto, A. (Ana) Cenoz, S. (Santiago) Pérez, I. (Isidoro) Abdulkarim, M. (Muthanna) Gumbleton, M. (Mark) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) |
| author_sort | Matías, J. (Jose) |
| collection | DSpace |
| description | Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of illness and death in
neonatal and recently weaned pigs. The immune protection of the piglets derives from maternal
colostrum, since this species does not receive maternal antibodies through the placenta. In the
present study, outer membrane vesicles (OMVs) obtained from main ETEC strains involved in
piglet infection (F4 and F18 serotypes), encapsulated into zein nanoparticles coated with Gantrez®®
AN-mannosamine conjugate, were used to orally immunize mice and pregnant sows. Loaded
nanoparticles were homogeneous and spherical in a shape, with a size of 220–280 nm. The diffusion of
nanoparticles through porcine intestinal mucus barrier was assessed by a Multiple Particle Tracking
technique, showing that these particles were able to diffuse efficiently (1.3% diffusion coefficient),
validating their oral use. BALB/c mice were either orally immunized with free OMVs or encapsulated
into nanoparticles (100 µg OMVs/mouse). Results indicated that a single dose of loaded nanoparticles
was able to elicit higher levels of serum specific IgG1, IgG2a and IgA, as well as intestinal IgA, with
respect to the free antigens. In addition, nanoparticles induced an increase in levels of IL-2, IL-4 and
IFN-γ with respect to the administration of free OMVs. Orally immunized pregnant sows with the
same formulation elicited colostrum-, serum- (IgG, IgA or IgM) and fecal- (IgA) specific antibodies
and, what is most relevant, offspring suckling piglets presented specific IgG in serum. Further studies
are needed to determine the infection protective capacity of this new oral subunit vaccine |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-67202 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2023 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-672022023-09-04T05:12:00Z Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles Matías, J. (Jose) Brotons-Canto, A. (Ana) Cenoz, S. (Santiago) Pérez, I. (Isidoro) Abdulkarim, M. (Muthanna) Gumbleton, M. (Mark) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) Vaccine Outer membrane vesicles ETEC Escherichia coli Nanoparticles Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of illness and death in neonatal and recently weaned pigs. The immune protection of the piglets derives from maternal colostrum, since this species does not receive maternal antibodies through the placenta. In the present study, outer membrane vesicles (OMVs) obtained from main ETEC strains involved in piglet infection (F4 and F18 serotypes), encapsulated into zein nanoparticles coated with Gantrez®® AN-mannosamine conjugate, were used to orally immunize mice and pregnant sows. Loaded nanoparticles were homogeneous and spherical in a shape, with a size of 220–280 nm. The diffusion of nanoparticles through porcine intestinal mucus barrier was assessed by a Multiple Particle Tracking technique, showing that these particles were able to diffuse efficiently (1.3% diffusion coefficient), validating their oral use. BALB/c mice were either orally immunized with free OMVs or encapsulated into nanoparticles (100 µg OMVs/mouse). Results indicated that a single dose of loaded nanoparticles was able to elicit higher levels of serum specific IgG1, IgG2a and IgA, as well as intestinal IgA, with respect to the free antigens. In addition, nanoparticles induced an increase in levels of IL-2, IL-4 and IFN-γ with respect to the administration of free OMVs. Orally immunized pregnant sows with the same formulation elicited colostrum-, serum- (IgG, IgA or IgM) and fecal- (IgA) specific antibodies and, what is most relevant, offspring suckling piglets presented specific IgG in serum. Further studies are needed to determine the infection protective capacity of this new oral subunit vaccine 2023-08-31T08:48:30Z 2023-08-31T08:48:30Z 2020 info:eu-repo/semantics/article https://hdl.handle.net/10171/67202 eng info:eu-repo/grantAgreement/MINECO/Retos Colaboración: Proyectos de I+D+i/RTC-2014-2004-2/ES/Control sanitario integral de explotaciones porcinas info:eu-repo/semantics/openAccess application/pdf MDPI AG |
| spellingShingle | Vaccine Outer membrane vesicles ETEC Escherichia coli Nanoparticles Matías, J. (Jose) Brotons-Canto, A. (Ana) Cenoz, S. (Santiago) Pérez, I. (Isidoro) Abdulkarim, M. (Muthanna) Gumbleton, M. (Mark) Irache, J.M. (Juan Manuel) Gamazo, C. (Carlos) Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles |
| title | Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles |
| title_full | Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles |
| title_fullStr | Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles |
| title_full_unstemmed | Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles |
| title_short | Oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles |
| title_sort | oral immunogenicity in mice and sows of enterotoxigenic escherichia coli outer-membrane vesicles incorporated into zein-based nanoparticles |
| topic | Vaccine Outer membrane vesicles ETEC Escherichia coli Nanoparticles |
| url | https://hdl.handle.net/10171/67202 |
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