Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections
Urinary tract infections (UTI) are highly prevalent in nosocomial and community settings, and their diagnosis is costly and time-consuming. Screening methods represent an important advance towards the final UTI diagnosis, diminishing inappropriate treatment or clinical complications. Automated ana...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Elsevier
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/68856 |
| _version_ | 1793400026451410944 |
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| author | Iñigo, M. (Melania) Coello, A. (Andreu) Fernández-Rivas, G. (Gema) Carrasco, M. (María) Marcó, C. (Clara) Fernández, A. (Anabel) Casamajor, T. (Teresa) Ausina, V. (Vicente) |
| author_facet | Iñigo, M. (Melania) Coello, A. (Andreu) Fernández-Rivas, G. (Gema) Carrasco, M. (María) Marcó, C. (Clara) Fernández, A. (Anabel) Casamajor, T. (Teresa) Ausina, V. (Vicente) |
| author_sort | Iñigo, M. (Melania) |
| collection | DSpace |
| description | Urinary tract infections (UTI) are highly prevalent in nosocomial and community settings, and their diagnosis is
costly and time-consuming. Screening methods represent an important advance towards the final UTI diagnosis,
diminishing inappropriate treatment or clinical complications. Automated analyzers have been developed and
commercialized to screen and rule out negative urine samples. The aim of this study was to evaluate two of
these automated analyzers (SediMax, an automatic sediment analyzer and UF-1000i a flow cytometer) to predict
negative urine cultures. A total of 1934 urine samples were analyzed. A very strong correlation for white blood
cells (WBC) (rs: 0.928) and a strong correlation for bacteria (BAC) (rs: 0.693) were obtained. We also calculated
optimal cut-off points for both autoanalyzers: 18 WBC/μL and 97 BAC/μL for SediMax (sensitivity = 96.25%,
specificity = 63.04%, negative predictive value = 97.97%), and 40 WBC/μL and 460 BAC/μL for UF-1000i
(sensitivity = 98.13%, specificity = 79.16%, negative predictive value = 99.18%). The use of SediMax and UF-
1000i resulted in a 46.33% and 57.19% reduction of all samples cultured, respectively. In conclusion, both ana-
lyzers are good UTI screening tools in our setting. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-68856 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-688562024-02-12T06:07:40Z Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections Iñigo, M. (Melania) Coello, A. (Andreu) Fernández-Rivas, G. (Gema) Carrasco, M. (María) Marcó, C. (Clara) Fernández, A. (Anabel) Casamajor, T. (Teresa) Ausina, V. (Vicente) Materias Investigacion::Ciencias de la Salud Urinary tract infection Urinalysis Autoanalyzers Flow cytometry Automated urine sediment Urinary tract infections (UTI) are highly prevalent in nosocomial and community settings, and their diagnosis is costly and time-consuming. Screening methods represent an important advance towards the final UTI diagnosis, diminishing inappropriate treatment or clinical complications. Automated analyzers have been developed and commercialized to screen and rule out negative urine samples. The aim of this study was to evaluate two of these automated analyzers (SediMax, an automatic sediment analyzer and UF-1000i a flow cytometer) to predict negative urine cultures. A total of 1934 urine samples were analyzed. A very strong correlation for white blood cells (WBC) (rs: 0.928) and a strong correlation for bacteria (BAC) (rs: 0.693) were obtained. We also calculated optimal cut-off points for both autoanalyzers: 18 WBC/μL and 97 BAC/μL for SediMax (sensitivity = 96.25%, specificity = 63.04%, negative predictive value = 97.97%), and 40 WBC/μL and 460 BAC/μL for UF-1000i (sensitivity = 98.13%, specificity = 79.16%, negative predictive value = 99.18%). The use of SediMax and UF- 1000i resulted in a 46.33% and 57.19% reduction of all samples cultured, respectively. In conclusion, both ana- lyzers are good UTI screening tools in our setting. 2024-02-07T07:53:44Z 2024-02-07T07:53:44Z 2016 info:eu-repo/semantics/article https://hdl.handle.net/10171/68856 eng info:eu-repo/semantics/closedAccess application/pdf Elsevier |
| spellingShingle | Materias Investigacion::Ciencias de la Salud Urinary tract infection Urinalysis Autoanalyzers Flow cytometry Automated urine sediment Iñigo, M. (Melania) Coello, A. (Andreu) Fernández-Rivas, G. (Gema) Carrasco, M. (María) Marcó, C. (Clara) Fernández, A. (Anabel) Casamajor, T. (Teresa) Ausina, V. (Vicente) Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections |
| title | Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections |
| title_full | Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections |
| title_fullStr | Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections |
| title_full_unstemmed | Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections |
| title_short | Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections |
| title_sort | evaluation of the sedimax automated microscopy sediment analyzer and the sysmex uf-1000i flow cytometer as screening tools to rule out negative urinary tract infections |
| topic | Materias Investigacion::Ciencias de la Salud Urinary tract infection Urinalysis Autoanalyzers Flow cytometry Automated urine sediment |
| url | https://hdl.handle.net/10171/68856 |
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