Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based...
| Main Authors: | , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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MDPI
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/12789 |
| _version_ | 1789406334838374400 |
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| author | Vuletin Selak, Gabriela Baruca Arbeiter, Alenka Cuevas González, Julián Perica, Slavko Pujic, Petar Raboteg Božiković, Marina Bandelj, Dunja |
| author_facet | Vuletin Selak, Gabriela Baruca Arbeiter, Alenka Cuevas González, Julián Perica, Slavko Pujic, Petar Raboteg Božiković, Marina Bandelj, Dunja |
| author_sort | Vuletin Selak, Gabriela |
| collection | DSpace |
| description | The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-12789 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2021 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-127892023-04-12T18:53:15Z Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards Vuletin Selak, Gabriela Baruca Arbeiter, Alenka Cuevas González, Julián Perica, Slavko Pujic, Petar Raboteg Božiković, Marina Bandelj, Dunja Olea europaea L. cultivar ‘Oblica’ microsatellites seed paternity assignment cross-compatibility self-incompatibility The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings. 2021-11-15T12:16:06Z 2021-11-15T12:16:06Z 2021-10-31 info:eu-repo/semantics/article 2223-7747 http://hdl.handle.net/10835/12789 10.3390/plants10112356 en https://www.mdpi.com/2223-7747/10/11/2356 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | Olea europaea L. cultivar ‘Oblica’ microsatellites seed paternity assignment cross-compatibility self-incompatibility Vuletin Selak, Gabriela Baruca Arbeiter, Alenka Cuevas González, Julián Perica, Slavko Pujic, Petar Raboteg Božiković, Marina Bandelj, Dunja Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards |
| title | Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards |
| title_full | Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards |
| title_fullStr | Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards |
| title_full_unstemmed | Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards |
| title_short | Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards |
| title_sort | seed paternity analysis using ssr markers to assess successful pollen donors in mixed olive orchards |
| topic | Olea europaea L. cultivar ‘Oblica’ microsatellites seed paternity assignment cross-compatibility self-incompatibility |
| url | http://hdl.handle.net/10835/12789 |
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