Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity
Lipids are one of the primary metabolites of microalgae and cyanobacteria, which enrich their utility in the pharmaceutical, feed, cosmetic, and chemistry sectors. This work describes the isolation, structural elucidation, and the antibiotic and antibiofilm activities of diverse lipids produced by d...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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MDPI
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/13108 |
| _version_ | 1789406529431011328 |
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| author | Cepas, Virginio Gutiérrez-Del-Río, Ignacio López, Yuly Redondo-Blanco, Saúl Gabasa, Yaiza Iglesias Valdés-Solís, María José González Soengas, Raquel María Fernández-Lorenzo, Andrés López-Ibáñez, Sara Villar, Claudio J. Martins, Clara B. Ferreira, Joana D. Assunção, Mariana F. G. Santos, Lília M. A. Morais, João Castelo-Branco, Raquel Reis, Mariana A. Vasconcelos, Vitor López Ortiz, Fernando Lombó, Felipe Soto, Sara M. |
| author_facet | Cepas, Virginio Gutiérrez-Del-Río, Ignacio López, Yuly Redondo-Blanco, Saúl Gabasa, Yaiza Iglesias Valdés-Solís, María José González Soengas, Raquel María Fernández-Lorenzo, Andrés López-Ibáñez, Sara Villar, Claudio J. Martins, Clara B. Ferreira, Joana D. Assunção, Mariana F. G. Santos, Lília M. A. Morais, João Castelo-Branco, Raquel Reis, Mariana A. Vasconcelos, Vitor López Ortiz, Fernando Lombó, Felipe Soto, Sara M. |
| author_sort | Cepas, Virginio |
| collection | DSpace |
| description | Lipids are one of the primary metabolites of microalgae and cyanobacteria, which enrich their utility in the pharmaceutical, feed, cosmetic, and chemistry sectors. This work describes the isolation, structural elucidation, and the antibiotic and antibiofilm activities of diverse lipids produced by different microalgae and cyanobacteria strains from two European collections (ACOI and LEGE-CC). Three microalgae strains and one cyanobacteria strain were selected for their antibacterial and/or antibiofilm activity after the screening of about 600 strains carried out under the NoMorFilm European project. The total organic extracts were firstly fractionated using solid phase extraction methods, and the minimum inhibitory concentration and minimal biofilm inhibitory concentration against an array of human pathogens were determined. The isolation was carried out by bioassay-guided HPLC-DAD purification, and the structure of the isolated molecules responsible for the observed activities was determined by HPLC-HRESIMS and NMR methods. Sulfoquinovosyldiacylglycerol, monogalactosylmonoacylglycerol, sulfoquinovosylmonoacylglycerol, α-linolenic acid, hexadeca-4,7,10,13-tetraenoic acid (HDTA), palmitoleic acid, and lysophosphatidylcholine were found among the different active sub-fractions selected. In conclusion, cyanobacteria and microalgae produce a great variety of lipids with antibiotic and antibiofilm activity against the most important pathogens causing severe infections in humans. The use of these lipids in clinical treatments alone or in combination with antibiotics may provide an alternative to the current treatments. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-13108 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2021 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-131082023-04-12T19:47:26Z Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity Cepas, Virginio Gutiérrez-Del-Río, Ignacio López, Yuly Redondo-Blanco, Saúl Gabasa, Yaiza Iglesias Valdés-Solís, María José González Soengas, Raquel María Fernández-Lorenzo, Andrés López-Ibáñez, Sara Villar, Claudio J. Martins, Clara B. Ferreira, Joana D. Assunção, Mariana F. G. Santos, Lília M. A. Morais, João Castelo-Branco, Raquel Reis, Mariana A. Vasconcelos, Vitor López Ortiz, Fernando Lombó, Felipe Soto, Sara M. microalgae cyanobacteria free fatty acids glycoglycerolipids phospholipids antimicrobial antibiofilm human pathogens Lipids are one of the primary metabolites of microalgae and cyanobacteria, which enrich their utility in the pharmaceutical, feed, cosmetic, and chemistry sectors. This work describes the isolation, structural elucidation, and the antibiotic and antibiofilm activities of diverse lipids produced by different microalgae and cyanobacteria strains from two European collections (ACOI and LEGE-CC). Three microalgae strains and one cyanobacteria strain were selected for their antibacterial and/or antibiofilm activity after the screening of about 600 strains carried out under the NoMorFilm European project. The total organic extracts were firstly fractionated using solid phase extraction methods, and the minimum inhibitory concentration and minimal biofilm inhibitory concentration against an array of human pathogens were determined. The isolation was carried out by bioassay-guided HPLC-DAD purification, and the structure of the isolated molecules responsible for the observed activities was determined by HPLC-HRESIMS and NMR methods. Sulfoquinovosyldiacylglycerol, monogalactosylmonoacylglycerol, sulfoquinovosylmonoacylglycerol, α-linolenic acid, hexadeca-4,7,10,13-tetraenoic acid (HDTA), palmitoleic acid, and lysophosphatidylcholine were found among the different active sub-fractions selected. In conclusion, cyanobacteria and microalgae produce a great variety of lipids with antibiotic and antibiofilm activity against the most important pathogens causing severe infections in humans. The use of these lipids in clinical treatments alone or in combination with antibiotics may provide an alternative to the current treatments. 2021-12-16T18:38:13Z 2021-12-16T18:38:13Z 2021-11-27 info:eu-repo/semantics/article 1660-3397 http://hdl.handle.net/10835/13108 10.3390/md19120675 en https://www.mdpi.com/1660-3397/19/12/675 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | microalgae cyanobacteria free fatty acids glycoglycerolipids phospholipids antimicrobial antibiofilm human pathogens Cepas, Virginio Gutiérrez-Del-Río, Ignacio López, Yuly Redondo-Blanco, Saúl Gabasa, Yaiza Iglesias Valdés-Solís, María José González Soengas, Raquel María Fernández-Lorenzo, Andrés López-Ibáñez, Sara Villar, Claudio J. Martins, Clara B. Ferreira, Joana D. Assunção, Mariana F. G. Santos, Lília M. A. Morais, João Castelo-Branco, Raquel Reis, Mariana A. Vasconcelos, Vitor López Ortiz, Fernando Lombó, Felipe Soto, Sara M. Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity |
| title | Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity |
| title_full | Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity |
| title_fullStr | Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity |
| title_full_unstemmed | Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity |
| title_short | Microalgae and Cyanobacteria Strains as Producers of Lipids with Antibacterial and Antibiofilm Activity |
| title_sort | microalgae and cyanobacteria strains as producers of lipids with antibacterial and antibiofilm activity |
| topic | microalgae cyanobacteria free fatty acids glycoglycerolipids phospholipids antimicrobial antibiofilm human pathogens |
| url | http://hdl.handle.net/10835/13108 |
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