Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds

Extraction of carotenoids and fatty acids from microalgae is a technological bottleneck in processing. An improved extraction process was developed to scale the production of these bioproducts from Nannochloropsis gaditana. Different cell disruption methods were evaluated in terms of carotenoids...

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Main Authors: Sales, Rafael, Cerón-García, María del Carmen, Navaro-López, Elvira, González-López, Cynthia Victoria, Yumi Tsuzuki, Mônica, Acién Fernández, Francisco Gabriel, Alarcón López, Francisco Javier, Molina-Grima, Emilio
Format: info:eu-repo/semantics/article
Language:English
Published: 2022
Online Access:http://hdl.handle.net/10835/13617
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author Sales, Rafael
Cerón-García, María del Carmen
Navaro-López, Elvira
González-López, Cynthia Victoria
Yumi Tsuzuki, Mônica
Acién Fernández, Francisco Gabriel
Alarcón López, Francisco Javier
Molina-Grima, Emilio
author_facet Sales, Rafael
Cerón-García, María del Carmen
Navaro-López, Elvira
González-López, Cynthia Victoria
Yumi Tsuzuki, Mônica
Acién Fernández, Francisco Gabriel
Alarcón López, Francisco Javier
Molina-Grima, Emilio
author_sort Sales, Rafael
collection DSpace
description Extraction of carotenoids and fatty acids from microalgae is a technological bottleneck in processing. An improved extraction process was developed to scale the production of these bioproducts from Nannochloropsis gaditana. Different cell disruption methods were evaluated in terms of carotenoids release. Ethanol was substituted by isopropyl alcohol in a three-component solution of water:isopropyl alcohol:hexane (WIH), in which the extracts were separated by solution partitioning. This resulted in higher carotenoid and fatty acid recovery yields if compared to the standard method. The extraction method was replicated on a pilot scale obtaining similar carotenoid recovery yields, higher than those of the standard method. Although fatty acid recovery was lower than that of the small-scale tests, yields above 85% were obtained. This demonstrated that the method was scalable for the extraction of high- value products from microalgae up to 10 L reactor volume. The use of isopropyl alcohol, which is cheaper than ethanol, and the separation of the solution phases by partitioning (avoiding drying) could contribute to reduce operation costs of downstream processing.
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spelling oai:repositorio.ual.es:10835-136172023-10-11T11:12:46Z Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds Sales, Rafael Cerón-García, María del Carmen Navaro-López, Elvira González-López, Cynthia Victoria Yumi Tsuzuki, Mônica Acién Fernández, Francisco Gabriel Alarcón López, Francisco Javier Molina-Grima, Emilio Extraction of carotenoids and fatty acids from microalgae is a technological bottleneck in processing. An improved extraction process was developed to scale the production of these bioproducts from Nannochloropsis gaditana. Different cell disruption methods were evaluated in terms of carotenoids release. Ethanol was substituted by isopropyl alcohol in a three-component solution of water:isopropyl alcohol:hexane (WIH), in which the extracts were separated by solution partitioning. This resulted in higher carotenoid and fatty acid recovery yields if compared to the standard method. The extraction method was replicated on a pilot scale obtaining similar carotenoid recovery yields, higher than those of the standard method. Although fatty acid recovery was lower than that of the small-scale tests, yields above 85% were obtained. This demonstrated that the method was scalable for the extraction of high- value products from microalgae up to 10 L reactor volume. The use of isopropyl alcohol, which is cheaper than ethanol, and the separation of the solution phases by partitioning (avoiding drying) could contribute to reduce operation costs of downstream processing. 2022-04-19T11:09:12Z 2022-04-19T11:09:12Z 2020-03-06 info:eu-repo/semantics/article http://hdl.handle.net/10835/13617 en http://eu-repo/grantAgreement/ES/MINECO/2016SABANA/ES/Sustainable%20Algae%20Biorefinery%20for%20Agriculture%20aNd%20Aquaculture/SABANA/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess
spellingShingle Sales, Rafael
Cerón-García, María del Carmen
Navaro-López, Elvira
González-López, Cynthia Victoria
Yumi Tsuzuki, Mônica
Acién Fernández, Francisco Gabriel
Alarcón López, Francisco Javier
Molina-Grima, Emilio
Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds
title Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds
title_full Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds
title_fullStr Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds
title_full_unstemmed Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds
title_short Processing Nannochloropsis gaditana biomass for the extraction of high value biocompounds
title_sort processing nannochloropsis gaditana biomass for the extraction of high value biocompounds
url http://hdl.handle.net/10835/13617
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