Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy
Pulsed Electric Field (PEF) pre-treatment, applied on fresh microalgae Auxenochlorella protothecoides, induces spontaneous release of a substantial water fraction and enables subsequent lipid extraction using ethanol-hexane blends. In this study, fresh microalgae suspensions were treated with PEF...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
2022
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| Online Access: | http://hdl.handle.net/10835/13621 |
| _version_ | 1789406272739606528 |
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| author | Silve, Aude Chua, Boon Kian Papachristou, Ioannis Kubisch, Christin Nazarova, Natalja Wüstner, Rüdiger Leber, Klaus Frey, Wolfgang |
| author_facet | Silve, Aude Chua, Boon Kian Papachristou, Ioannis Kubisch, Christin Nazarova, Natalja Wüstner, Rüdiger Leber, Klaus Frey, Wolfgang |
| author_sort | Silve, Aude |
| collection | DSpace |
| description | Pulsed Electric Field (PEF) pre-treatment, applied on fresh microalgae Auxenochlorella
protothecoides, induces spontaneous release of a substantial water fraction and enables
subsequent lipid extraction using ethanol-hexane blends. In this study, fresh microalgae
suspensions were treated with PEF and incubated under inert conditions. Incubation promotes the
release of ions and carbohydrates and increases the yields of subsequent lipid extraction thus
enabling a considerable reduction of PEF-treatment energy. With a 20 hour incubation period at
25°C, almost total lipid extraction is achieved with a specific PEF-treatment energy of only
0.25 MJ/kg DW . Incubation on ice remains beneficial but less efficient than at 25°C. Additionally,
incubating microalgae cells in suspension at 100 g DW /L or in a dense paste, was almost equally
efficient. Correlation between the different results suggests that spontaneous release of ions and
carbohydrates facilitates more successful lipid extraction. A direct causality between the two
phenomena remains to be demonstrated. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-13621 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2022 |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-136212023-04-12T19:34:04Z Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy Silve, Aude Chua, Boon Kian Papachristou, Ioannis Kubisch, Christin Nazarova, Natalja Wüstner, Rüdiger Leber, Klaus Frey, Wolfgang Pulsed Electric Field (PEF) pre-treatment, applied on fresh microalgae Auxenochlorella protothecoides, induces spontaneous release of a substantial water fraction and enables subsequent lipid extraction using ethanol-hexane blends. In this study, fresh microalgae suspensions were treated with PEF and incubated under inert conditions. Incubation promotes the release of ions and carbohydrates and increases the yields of subsequent lipid extraction thus enabling a considerable reduction of PEF-treatment energy. With a 20 hour incubation period at 25°C, almost total lipid extraction is achieved with a specific PEF-treatment energy of only 0.25 MJ/kg DW . Incubation on ice remains beneficial but less efficient than at 25°C. Additionally, incubating microalgae cells in suspension at 100 g DW /L or in a dense paste, was almost equally efficient. Correlation between the different results suggests that spontaneous release of ions and carbohydrates facilitates more successful lipid extraction. A direct causality between the two phenomena remains to be demonstrated. 2022-04-19T11:26:34Z 2022-04-19T11:26:34Z 2020-11-01 info:eu-repo/semantics/article http://hdl.handle.net/10835/13621 en http://eu-repo/grantAgreement/ES/MINECO/2016SABANA/ES/Sustainable%20Algae%20Biorefinery%20for%20Agriculture%20aNd%20Aquaculture/SABANA/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| spellingShingle | Silve, Aude Chua, Boon Kian Papachristou, Ioannis Kubisch, Christin Nazarova, Natalja Wüstner, Rüdiger Leber, Klaus Frey, Wolfgang Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy |
| title | Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy |
| title_full | Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy |
| title_fullStr | Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy |
| title_full_unstemmed | Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy |
| title_short | Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy |
| title_sort | incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy |
| url | http://hdl.handle.net/10835/13621 |
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