Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media
Nitrogen is an essential nutrient for all life forms and its availability is periodically limited in marine and freshwater ecosystems. To optimize the growth of the freshwater microalga Chlamydopodium fusiforme and to investigate the cell acclimation process to different amounts of nitrogen, we g...
| Main Authors: | , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
2022
|
| Online Access: | http://hdl.handle.net/10835/13622 |
| _version_ | 1789406697841754112 |
|---|---|
| author | Touloupakis, Eleftherios Tartari, Gabriele Chini Zittelli, Graziella Torzillo, Giuseppe |
| author_facet | Touloupakis, Eleftherios Tartari, Gabriele Chini Zittelli, Graziella Torzillo, Giuseppe |
| author_sort | Touloupakis, Eleftherios |
| collection | DSpace |
| description | Nitrogen is an essential nutrient for all life forms and its availability is periodically limited in
marine and freshwater ecosystems. To optimize the growth of the freshwater microalga
Chlamydopodium fusiforme and to investigate the cell acclimation process to different amounts
of nitrogen, we grew cells in Bristol and BG11, under fixed conditions of 10:14 light/dark cycle.
Cultures grown in BG11 exhibited higher biomass accumulation, reaching a cell dry weight of
1.94±0.1 g L
-1
, while in Bristol it reached 1.58±0.1 g L
-1
. In cells grown in BG11 chlorophyll
content increased continuously up to 79.6 mg L
-1 (9th day), while in Bristol was much lower
(29.5 mg L
-1
). This fact caused a strong difference in their chlorophyll optical cross-sections
and consequently in their photosynthetic performance (oxygen evolution and electron transport
rate). Carotenoid content followed a similar pattern. It was interesting to note that despite this
great difference in chlorophyll concentration, both photosynthesis rate and cell dry weight were
not affected proportionally. The chlorophyll a fluorescence measurements indicated a stable
effective quantum yield of photosystem II in both culture conditions. During the dark phase of
the cycle we observed in both cultures a remarkable reduction of both electron transfer and
photosynthesis rates, while maximum quantum yield of PSII remained stable (0.74±0.01). This
indicated that the reduction in both photosynthesis rate and ETRmax observed during the dark
phase were not due to damage of PSII reaction centers but rather to reduced flow of electron
subsequent to PSII. Recovery of the fully photosynthetic capacity required about one hour of
illumination. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-13622 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2022 |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-136222023-04-12T19:35:47Z Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media Touloupakis, Eleftherios Tartari, Gabriele Chini Zittelli, Graziella Torzillo, Giuseppe Nitrogen is an essential nutrient for all life forms and its availability is periodically limited in marine and freshwater ecosystems. To optimize the growth of the freshwater microalga Chlamydopodium fusiforme and to investigate the cell acclimation process to different amounts of nitrogen, we grew cells in Bristol and BG11, under fixed conditions of 10:14 light/dark cycle. Cultures grown in BG11 exhibited higher biomass accumulation, reaching a cell dry weight of 1.94±0.1 g L -1 , while in Bristol it reached 1.58±0.1 g L -1 . In cells grown in BG11 chlorophyll content increased continuously up to 79.6 mg L -1 (9th day), while in Bristol was much lower (29.5 mg L -1 ). This fact caused a strong difference in their chlorophyll optical cross-sections and consequently in their photosynthetic performance (oxygen evolution and electron transport rate). Carotenoid content followed a similar pattern. It was interesting to note that despite this great difference in chlorophyll concentration, both photosynthesis rate and cell dry weight were not affected proportionally. The chlorophyll a fluorescence measurements indicated a stable effective quantum yield of photosystem II in both culture conditions. During the dark phase of the cycle we observed in both cultures a remarkable reduction of both electron transfer and photosynthesis rates, while maximum quantum yield of PSII remained stable (0.74±0.01). This indicated that the reduction in both photosynthesis rate and ETRmax observed during the dark phase were not due to damage of PSII reaction centers but rather to reduced flow of electron subsequent to PSII. Recovery of the fully photosynthetic capacity required about one hour of illumination. 2022-04-19T11:26:49Z 2022-04-19T11:26:49Z 2020-03-29 info:eu-repo/semantics/article http://hdl.handle.net/10835/13622 en http://eu-repo/grantAgreement/ES/MINECO/2016SABANA/ES/Sustainable%20Algae%20Biorefinery%20for%20Agriculture%20aNd%20Aquaculture/SABANA/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| spellingShingle | Touloupakis, Eleftherios Tartari, Gabriele Chini Zittelli, Graziella Torzillo, Giuseppe Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media |
| title | Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media |
| title_full | Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media |
| title_fullStr | Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media |
| title_full_unstemmed | Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media |
| title_short | Growth and photosynthetic performance of Chlamydopodium fusiforme cells cultivated in BG and Bristol media |
| title_sort | growth and photosynthetic performance of chlamydopodium fusiforme cells cultivated in bg and bristol media |
| url | http://hdl.handle.net/10835/13622 |
| work_keys_str_mv | AT touloupakiseleftherios growthandphotosyntheticperformanceofchlamydopodiumfusiformecellscultivatedinbgandbristolmedia AT tartarigabriele growthandphotosyntheticperformanceofchlamydopodiumfusiformecellscultivatedinbgandbristolmedia AT chinizittelligraziella growthandphotosyntheticperformanceofchlamydopodiumfusiformecellscultivatedinbgandbristolmedia AT torzillogiuseppe growthandphotosyntheticperformanceofchlamydopodiumfusiformecellscultivatedinbgandbristolmedia |