The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction
The two main methods for partitioning crude methanolic extract from Amphidinium carterae biomass were compared. The objective was to obtain three enriched fractions containing amphidinols (APDs), carotenoids, and fatty acids. Since the most valuable bioproducts are APDs, their recovery was the princ...
| Main Authors: | , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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MDPI
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/13956 |
| _version_ | 1789406274287304704 |
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| author | López Rodríguez, Mercedes López Rosales, Lorenzo Diletta, Giullia Cerón García, María del Carmen Navarro López, Elvira Gallardo Rodríguez, Juan José Tristán, Ana Isabel Ralha Abreu, Ana Cristina García Camacho, Francisco |
| author_facet | López Rodríguez, Mercedes López Rosales, Lorenzo Diletta, Giullia Cerón García, María del Carmen Navarro López, Elvira Gallardo Rodríguez, Juan José Tristán, Ana Isabel Ralha Abreu, Ana Cristina García Camacho, Francisco |
| author_sort | López Rodríguez, Mercedes |
| collection | DSpace |
| description | The two main methods for partitioning crude methanolic extract from Amphidinium carterae biomass were compared. The objective was to obtain three enriched fractions containing amphidinols (APDs), carotenoids, and fatty acids. Since the most valuable bioproducts are APDs, their recovery was the principal goal. The first method consisted of a solid-phase extraction (SPE) in reverse phase that, for the first time, was optimized to fractionate organic methanolic extracts from Amphidinium carterae biomass using reverse-phase C18 as the adsorbent. The second method consisted of a two-step liquid-liquid extraction coupled with SPE and, alternatively, with solvent partitioning. The SPE method allowed the recovery of the biologically-active fraction (containing the APDs) by eluting with methanol (MeOH): water (H2O) (80:20 v/v). Alternatively, an APD purification strategy using solvent partitioning proved to be a better approach for providing APDs in a clear-cut way. When using n-butanol, APDs were obtained at a 70% concentration (w/w), whereas for the SPE method, the most concentrated fraction was only 18% (w/w). For the other fractions (carotenoids and fatty acids), a two-step liquid-liquid extraction (LLE) method coupled with the solvent partitioning method presented the best results. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-13956 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-139562023-04-12T19:34:10Z The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction López Rodríguez, Mercedes López Rosales, Lorenzo Diletta, Giullia Cerón García, María del Carmen Navarro López, Elvira Gallardo Rodríguez, Juan José Tristán, Ana Isabel Ralha Abreu, Ana Cristina García Camacho, Francisco dinoflagellate solid-phase extraction liquid-liquid extraction amphidinol fatty acids carotenoids The two main methods for partitioning crude methanolic extract from Amphidinium carterae biomass were compared. The objective was to obtain three enriched fractions containing amphidinols (APDs), carotenoids, and fatty acids. Since the most valuable bioproducts are APDs, their recovery was the principal goal. The first method consisted of a solid-phase extraction (SPE) in reverse phase that, for the first time, was optimized to fractionate organic methanolic extracts from Amphidinium carterae biomass using reverse-phase C18 as the adsorbent. The second method consisted of a two-step liquid-liquid extraction coupled with SPE and, alternatively, with solvent partitioning. The SPE method allowed the recovery of the biologically-active fraction (containing the APDs) by eluting with methanol (MeOH): water (H2O) (80:20 v/v). Alternatively, an APD purification strategy using solvent partitioning proved to be a better approach for providing APDs in a clear-cut way. When using n-butanol, APDs were obtained at a 70% concentration (w/w), whereas for the SPE method, the most concentrated fraction was only 18% (w/w). For the other fractions (carotenoids and fatty acids), a two-step liquid-liquid extraction (LLE) method coupled with the solvent partitioning method presented the best results. 2022-09-13T15:43:16Z 2022-09-13T15:43:16Z 2022-08-28 info:eu-repo/semantics/article 2072-6651 http://hdl.handle.net/10835/13956 10.3390/toxins14090593 en https://www.mdpi.com/2072-6651/14/9/593 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | dinoflagellate solid-phase extraction liquid-liquid extraction amphidinol fatty acids carotenoids López Rodríguez, Mercedes López Rosales, Lorenzo Diletta, Giullia Cerón García, María del Carmen Navarro López, Elvira Gallardo Rodríguez, Juan José Tristán, Ana Isabel Ralha Abreu, Ana Cristina García Camacho, Francisco The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction |
| title | The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction |
| title_full | The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction |
| title_fullStr | The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction |
| title_full_unstemmed | The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction |
| title_short | The Isolation of Specialty Compounds from Amphidinium carterae Biomass by Two-Step Solid-Phase and Liquid-Liquid Extraction |
| title_sort | isolation of specialty compounds from amphidinium carterae biomass by two-step solid-phase and liquid-liquid extraction |
| topic | dinoflagellate solid-phase extraction liquid-liquid extraction amphidinol fatty acids carotenoids |
| url | http://hdl.handle.net/10835/13956 |
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