Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors
Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain. The ponds were used for continuous, all-year-round microalg...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
MDPI
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/14021 |
| _version_ | 1789406488113971200 |
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| author | Masojídek, Jiri Gómez Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sánchez Zurano, Ana Silva Benavides, Ana Margarita Barceló Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe López Figueroa, Félix Acién Fernández, Francisco Gabriel |
| author_facet | Masojídek, Jiri Gómez Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sánchez Zurano, Ana Silva Benavides, Ana Margarita Barceló Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe López Figueroa, Félix Acién Fernández, Francisco Gabriel |
| author_sort | Masojídek, Jiri |
| collection | DSpace |
| description | Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO2 nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62–0.71), and relative electron transport rate rETR between 200 and 450 μmol e− m−2 s−1 at midday, which resulted in biomass productivity of about 15–25 g DW m−2 day−1. The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-14021 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-140212023-04-12T19:35:04Z Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors Masojídek, Jiri Gómez Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sánchez Zurano, Ana Silva Benavides, Ana Margarita Barceló Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe López Figueroa, Félix Acién Fernández, Francisco Gabriel biomass biostimulanting activity chlorophyll fluorescence microalga Micractinium oxygen production photosynthesis raceway pond large-scale bioreactor wastewater Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO2 nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62–0.71), and relative electron transport rate rETR between 200 and 450 μmol e− m−2 s−1 at midday, which resulted in biomass productivity of about 15–25 g DW m−2 day−1. The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes. 2022-10-05T17:52:47Z 2022-10-05T17:52:47Z 2022-09-22 info:eu-repo/semantics/article 2079-7737 http://hdl.handle.net/10835/14021 10.3390/biology11101380 en https://www.mdpi.com/2079-7737/11/10/1380 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | biomass biostimulanting activity chlorophyll fluorescence microalga Micractinium oxygen production photosynthesis raceway pond large-scale bioreactor wastewater Masojídek, Jiri Gómez Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sánchez Zurano, Ana Silva Benavides, Ana Margarita Barceló Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe López Figueroa, Félix Acién Fernández, Francisco Gabriel Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
| title | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
| title_full | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
| title_fullStr | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
| title_full_unstemmed | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
| title_short | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
| title_sort | photosynthesis monitoring in microalgae cultures grown on municipal wastewater as a nutrient source in large-scale outdoor bioreactors |
| topic | biomass biostimulanting activity chlorophyll fluorescence microalga Micractinium oxygen production photosynthesis raceway pond large-scale bioreactor wastewater |
| url | http://hdl.handle.net/10835/14021 |
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