Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry
A new polyvalent wide-scope analytical method, valid for both raw and processed (juices) fruits, combining target and non-target strategies, has been developed and validated to determine low concentrations of 260 pesticides, as well as many potential non-target substances and metabolites. The target...
| Main Authors: | , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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MDPI
2023
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| Online Access: | http://hdl.handle.net/10835/14235 |
| _version_ | 1789406339906142208 |
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| author | Granados Povedano, Mireya Dóminguez Pérez, Irene Egea González, Francisco Javier Garrido Frenich, Antonia Arrebola Liébanas, Francisco Javier |
| author_facet | Granados Povedano, Mireya Dóminguez Pérez, Irene Egea González, Francisco Javier Garrido Frenich, Antonia Arrebola Liébanas, Francisco Javier |
| author_sort | Granados Povedano, Mireya |
| collection | DSpace |
| description | A new polyvalent wide-scope analytical method, valid for both raw and processed (juices) fruits, combining target and non-target strategies, has been developed and validated to determine low concentrations of 260 pesticides, as well as many potential non-target substances and metabolites. The target approach has been validated according to SANTE Guide requirements. Trueness, precision, linearity, and robustness values were validated in raw fruit (apple) and juice (apple juice) as representative solid and liquid food commodities. Recoveries were between 70–120% and two ranges of linearity were observed: 0.5–20 μg kg−1 (0.5–20 μg L−1 apple juice) and 20–100 μg kg−1 (20–100 μg L−1 apple juice). The limits of quantification (LOQs) reached were lower than 0.2 μg kg−1 in apple (0.2 μg L−1 apple juice) in most cases. The developed method, based on QuEChERS extraction followed by gas chromatography-high resolution mass spectrometry (GC-HRMS), achieves part-per-trillions lower limits, which allowed the detection of 18 pesticides in commercial samples. The non-target approach is based on a retrospective analysis of suspect compounds, which has been optimized to detect up to 25 additional compounds, increasing the scope of the method. This made it possible to confirm the presence of two pesticide metabolites which were not considered in the target screening, phtamlimide and tetrahydrophthalimide. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-14235 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2023 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-142352023-04-12T19:46:58Z Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry Granados Povedano, Mireya Dóminguez Pérez, Irene Egea González, Francisco Javier Garrido Frenich, Antonia Arrebola Liébanas, Francisco Javier GC-Q-Exactive Orbitrap MS juices fruits ultra-trace analysis pesticide residues retrospective analysis A new polyvalent wide-scope analytical method, valid for both raw and processed (juices) fruits, combining target and non-target strategies, has been developed and validated to determine low concentrations of 260 pesticides, as well as many potential non-target substances and metabolites. The target approach has been validated according to SANTE Guide requirements. Trueness, precision, linearity, and robustness values were validated in raw fruit (apple) and juice (apple juice) as representative solid and liquid food commodities. Recoveries were between 70–120% and two ranges of linearity were observed: 0.5–20 μg kg−1 (0.5–20 μg L−1 apple juice) and 20–100 μg kg−1 (20–100 μg L−1 apple juice). The limits of quantification (LOQs) reached were lower than 0.2 μg kg−1 in apple (0.2 μg L−1 apple juice) in most cases. The developed method, based on QuEChERS extraction followed by gas chromatography-high resolution mass spectrometry (GC-HRMS), achieves part-per-trillions lower limits, which allowed the detection of 18 pesticides in commercial samples. The non-target approach is based on a retrospective analysis of suspect compounds, which has been optimized to detect up to 25 additional compounds, increasing the scope of the method. This made it possible to confirm the presence of two pesticide metabolites which were not considered in the target screening, phtamlimide and tetrahydrophthalimide. 2023-02-09T15:17:29Z 2023-02-09T15:17:29Z 2023-02-08 info:eu-repo/semantics/article 2304-8158 http://hdl.handle.net/10835/14235 10.3390/foods12040739 en https://www.mdpi.com/2304-8158/12/4/739 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | GC-Q-Exactive Orbitrap MS juices fruits ultra-trace analysis pesticide residues retrospective analysis Granados Povedano, Mireya Dóminguez Pérez, Irene Egea González, Francisco Javier Garrido Frenich, Antonia Arrebola Liébanas, Francisco Javier Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry |
| title | Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry |
| title_full | Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry |
| title_fullStr | Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry |
| title_full_unstemmed | Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry |
| title_short | Unified Method for Target and Non-Target Monitoring of Pesticide Residues in Fruits and Fruit Juices by Gas Chromatography-High Resolution Mass Spectrometry |
| title_sort | unified method for target and non-target monitoring of pesticide residues in fruits and fruit juices by gas chromatography-high resolution mass spectrometry |
| topic | GC-Q-Exactive Orbitrap MS juices fruits ultra-trace analysis pesticide residues retrospective analysis |
| url | http://hdl.handle.net/10835/14235 |
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