Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays
Despite the advance of knowledge about the factors and potential mechanisms triggering the ichthyotoxicity in microalgae, these remain unclear or are controversial for several species (e.g. Heterosigma). Neither typical toxicity tests carried out with cell extracts nor direct exposure to harmful...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
2023
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| Online Access: | http://hdl.handle.net/10835/14802 https://doi.org/10.1371/journal.pone.0201438 |
| _version_ | 1789406277521113088 |
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| author | Rivera, Alejandra Vega-Drake, Karina Aburto, Carla Cruzat, Fernando Ulloa, Viviana Caprile, Teresa Astuya-Villalón, Allisson Gallardo Rodríguez, Juan José |
| author_facet | Rivera, Alejandra Vega-Drake, Karina Aburto, Carla Cruzat, Fernando Ulloa, Viviana Caprile, Teresa Astuya-Villalón, Allisson Gallardo Rodríguez, Juan José |
| author_sort | Rivera, Alejandra |
| collection | DSpace |
| description | Despite the advance of knowledge about the factors and potential mechanisms triggering
the ichthyotoxicity in microalgae, these remain unclear or are controversial for several species
(e.g. Heterosigma). Neither typical toxicity tests carried out with cell extracts nor direct
exposure to harmful species were proved suitable to unravel the mechanism of harm.
Ichthyotoxic species show a complex harmful effect on fish, which is mediated through various
mechanisms depending on the species. In this work, we present a method to study sublethal
effects triggered by reactive oxygen species of a population of harmful algae in vivo
over a fish cell line. To that end, Transwell co-cultures in which causative and target species
are separated by a 0.4 μm pore membrane were carried out. This allowed the evaluation of
the effect of the released molecules by cells in a rapid and compact test. In our method, the
harmful effect was sensed through the transcriptional activation of sub-lethal marker
Hsp70b in the CHSE214 salmon cell line. The method was tested with the raphidophyte Heterosigma
akashiwo and Dunaliella tertiolecta (as negative control). It was shown that superoxide
intracellular content and its release are not linked in these species. The methodology
allowed proving that reactive oxygen species produced by H. akashiwo are able to induce
the transcriptional activation of sub-lethal marker Hsp70b. However, neither loss of viability
nor apoptosis was observed in CHSE214 salmon cell line except when exposed to direct
contact with the raphidophyte cells (or their extract). Consequently, ROS was not concluded
to be the main cause of ichthyotoxicity in H. akashiwo. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-14802 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2023 |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-148022023-12-13T13:44:57Z Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays Rivera, Alejandra Vega-Drake, Karina Aburto, Carla Cruzat, Fernando Ulloa, Viviana Caprile, Teresa Astuya-Villalón, Allisson Gallardo Rodríguez, Juan José Despite the advance of knowledge about the factors and potential mechanisms triggering the ichthyotoxicity in microalgae, these remain unclear or are controversial for several species (e.g. Heterosigma). Neither typical toxicity tests carried out with cell extracts nor direct exposure to harmful species were proved suitable to unravel the mechanism of harm. Ichthyotoxic species show a complex harmful effect on fish, which is mediated through various mechanisms depending on the species. In this work, we present a method to study sublethal effects triggered by reactive oxygen species of a population of harmful algae in vivo over a fish cell line. To that end, Transwell co-cultures in which causative and target species are separated by a 0.4 μm pore membrane were carried out. This allowed the evaluation of the effect of the released molecules by cells in a rapid and compact test. In our method, the harmful effect was sensed through the transcriptional activation of sub-lethal marker Hsp70b in the CHSE214 salmon cell line. The method was tested with the raphidophyte Heterosigma akashiwo and Dunaliella tertiolecta (as negative control). It was shown that superoxide intracellular content and its release are not linked in these species. The methodology allowed proving that reactive oxygen species produced by H. akashiwo are able to induce the transcriptional activation of sub-lethal marker Hsp70b. However, neither loss of viability nor apoptosis was observed in CHSE214 salmon cell line except when exposed to direct contact with the raphidophyte cells (or their extract). Consequently, ROS was not concluded to be the main cause of ichthyotoxicity in H. akashiwo. 2023-12-13T13:44:57Z 2023-12-13T13:44:57Z 2018-08-02 info:eu-repo/semantics/article http://hdl.handle.net/10835/14802 https://doi.org/10.1371/journal.pone.0201438 en Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| spellingShingle | Rivera, Alejandra Vega-Drake, Karina Aburto, Carla Cruzat, Fernando Ulloa, Viviana Caprile, Teresa Astuya-Villalón, Allisson Gallardo Rodríguez, Juan José Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays |
| title | Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays |
| title_full | Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays |
| title_fullStr | Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays |
| title_full_unstemmed | Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays |
| title_short | Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays |
| title_sort | study of the ichthyotoxic microalga heterosigma akashiwo by transcriptional activation of sublethal marker hsp70b in transwell co-culture assays |
| url | http://hdl.handle.net/10835/14802 https://doi.org/10.1371/journal.pone.0201438 |
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