Revisiting the succession of microbial populations throughout composting: A matter of thermotolerance
Composting has been traditionally considered a process in which a succession of mesophilic and thermophilic microbial populations occurs due to temperature changes. In order to deepen in this model, 1380 bacterial and fungal strains (the entire culturable microbiota isolated from a composting proc...
| Principais autores: | , , , , , , |
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| Formato: | info:eu-repo/semantics/article |
| Idioma: | English |
| Publicado em: |
2024
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| Assuntos: | |
| Acesso em linha: | http://hdl.handle.net/10835/15025 https://doi.org/10.1016/j.scitotenv.2021.145587 |
| Resumo: | Composting has been traditionally considered a process in which a succession of mesophilic and thermophilic
microbial populations occurs due to temperature changes. In order to deepen in this model, 1380 bacterial and
fungal strains (the entire culturable microbiota isolated from a composting process) were investigated for their
ability to grow across a wide range of temperatures (20 to 60 °C). First, qualitative tests were performed to establish a thermal profile for each strain. Then, quantitative tests allowed ascertaining the extent of growth for each
strain at each of the tested temperatures. The identity of the isolates enabled to position them taxonomically and
permitted tracking the strains throughout the process. Results showed that 90% of the isolates were classified as
thermotolerant (they grew at all tested temperatures). Only 9% and 1% of the studied strains showed to be strictly
mesophilic or thermophilic, respectively. Firmicutes exhibited the greatest thermal plasticity, followed by
Actinobacteria and Ascomycota. Most of the Proteobacteria and all Basidiomycota strains were also able to grow
at all the assayed temperatures. Thermotolerance was clearly demonstrated among the composting microbiota,
suggesting that the idea of the succession of mesophilic and thermophilic populations throughout the process
might need a reassessment. |
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