Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content
Fatty acid methyl esters (FAMEs, biodiesel) were produced from Nannochloropsis gaditana wet biomass (12% saponifiable lipids, SLs) by extraction of SLs and lipase catalyzed transesterification. Lipids were extracted by ethanol (96%)-hexane, and 31% pure SLs were obtained with 85% yield. When the lip...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/15030 |
| _version_ | 1789406318401945600 |
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| author | Navarro López, Elvira Robles Medina, Alfonso González Moreno, Pedro Antonio Jiménez Callejón, María José Esteban Cerdán, Luis Martín Valverde, Lorena Castillo López, Beatriz Molina Grima, Emilio |
| author_facet | Navarro López, Elvira Robles Medina, Alfonso González Moreno, Pedro Antonio Jiménez Callejón, María José Esteban Cerdán, Luis Martín Valverde, Lorena Castillo López, Beatriz Molina Grima, Emilio |
| author_sort | Navarro López, Elvira |
| collection | DSpace |
| description | Fatty acid methyl esters (FAMEs, biodiesel) were produced from Nannochloropsis gaditana wet biomass (12% saponifiable lipids, SLs) by extraction of SLs and lipase catalyzed transesterification. Lipids were extracted by ethanol (96%)-hexane, and 31% pure SLs were obtained with 85% yield. When the lipids were degummed, SL purity increased to 95%. Novozym 435 was selected from four lipases tested. Both the lipidic composition and the use of t-butanol instead of hexane increased the reaction velocity and the conversion, since both decreased due to the adsorption of polar lipids on the lipase immobilization support. The best FAME yield (94.7%) was attained at a reaction time of 48 h and using 10 mL of t-butanol/g SL, 0.225 g N435/ g SL, 11:1 methanol/SL molar ratio and adding the methanol in three steps. In these conditions the FAME conversion decreased by 9.8% after three reaction cycles catalyzed by the same lipase batch. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15030 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-150302024-01-10T08:38:07Z Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content Navarro López, Elvira Robles Medina, Alfonso González Moreno, Pedro Antonio Jiménez Callejón, María José Esteban Cerdán, Luis Martín Valverde, Lorena Castillo López, Beatriz Molina Grima, Emilio Biodiesel Microalga Lipase Transesterification Polar lipid Fatty acid methyl esters (FAMEs, biodiesel) were produced from Nannochloropsis gaditana wet biomass (12% saponifiable lipids, SLs) by extraction of SLs and lipase catalyzed transesterification. Lipids were extracted by ethanol (96%)-hexane, and 31% pure SLs were obtained with 85% yield. When the lipids were degummed, SL purity increased to 95%. Novozym 435 was selected from four lipases tested. Both the lipidic composition and the use of t-butanol instead of hexane increased the reaction velocity and the conversion, since both decreased due to the adsorption of polar lipids on the lipase immobilization support. The best FAME yield (94.7%) was attained at a reaction time of 48 h and using 10 mL of t-butanol/g SL, 0.225 g N435/ g SL, 11:1 methanol/SL molar ratio and adding the methanol in three steps. In these conditions the FAME conversion decreased by 9.8% after three reaction cycles catalyzed by the same lipase batch. 2024-01-10T08:38:07Z 2024-01-10T08:38:07Z 2015-07-01 info:eu-repo/semantics/article 09608524 http://hdl.handle.net/10835/15030 en info:eu-repo/semantics/openAccess |
| spellingShingle | Biodiesel Microalga Lipase Transesterification Polar lipid Navarro López, Elvira Robles Medina, Alfonso González Moreno, Pedro Antonio Jiménez Callejón, María José Esteban Cerdán, Luis Martín Valverde, Lorena Castillo López, Beatriz Molina Grima, Emilio Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content |
| title | Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content |
| title_full | Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content |
| title_fullStr | Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content |
| title_full_unstemmed | Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content |
| title_short | Enzymatic production of biodiesel from nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content |
| title_sort | enzymatic production of biodiesel from nannochloropsis gaditana lipids: influence of operational variables and polar lipid content |
| topic | Biodiesel Microalga Lipase Transesterification Polar lipid |
| url | http://hdl.handle.net/10835/15030 |
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