Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors
The aim of this work was to produce docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) enriched acylglycerols by alcoholysis of tuna and sardine oils, respectively, using isobutanol and 1-butanol as acyl-acceptors. The alcoholysis reactions were catalyzed by lipases Lipozyme® TL IM from Thermomyc...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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2024
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| Online Access: | https://doi.org/10.1016/j.bej.2014.08.010 http://hdl.handle.net/10835/15037 |
| _version_ | 1789406536264581120 |
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| author | Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Castillo López, Beatriz Robles Medina, Alfonso Navarro López, Elvira |
| author_facet | Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Castillo López, Beatriz Robles Medina, Alfonso Navarro López, Elvira |
| author_sort | Martín Valverde, Lorena |
| collection | DSpace |
| description | The aim of this work was to produce docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) enriched acylglycerols by alcoholysis of tuna and sardine oils, respectively, using isobutanol and 1-butanol as acyl-acceptors. The alcoholysis reactions were catalyzed by lipases Lipozyme® TL IM from Thermomyces lanuginosus and lipase QLG® from Alcaligenes sp., because these lipases have shown selectivity towards DHA and EPA, respectively. Studies were made to determine the influence of reaction time, alcohol/oil molar ratio, lipase amount and temperature. In the optimized conditions for the alcoholysis of tuna and sardine oils catalyzed by Lipozyme TL IM and lipase QLG, respectively, the DHA and EPA contents were trebled (from 22 to 69% for DHA, and from 19 to 61% for EPA). The stability of both lipases was also determined. Although Lipozyme TL IM is much more stable in isobutanol than in ethanol, with the former the conversion attained after four reaction cycles was about 40% of the initial conversion. In similar conditions, the conversion obtained with lipase QLG was about 88% of the initial conversion. In addition, the separation of DHA enriched acylglycerols and isobutyl esters from an alcoholysis reaction was studied by liquid–liquid fractionation using the ethanol–water–hexane biphasic system. The DHA enriched acylglycerols obtained were 97.6% pure (64.4% DHA). |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15037 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-150372024-01-10T08:46:39Z Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Castillo López, Beatriz Robles Medina, Alfonso Navarro López, Elvira Docosahexaenoic acid (DHA) Eicosapentaenoic acid (EPA) Lipase Biokinetic Enzyme deactivation Liquid-liquid extraction The aim of this work was to produce docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) enriched acylglycerols by alcoholysis of tuna and sardine oils, respectively, using isobutanol and 1-butanol as acyl-acceptors. The alcoholysis reactions were catalyzed by lipases Lipozyme® TL IM from Thermomyces lanuginosus and lipase QLG® from Alcaligenes sp., because these lipases have shown selectivity towards DHA and EPA, respectively. Studies were made to determine the influence of reaction time, alcohol/oil molar ratio, lipase amount and temperature. In the optimized conditions for the alcoholysis of tuna and sardine oils catalyzed by Lipozyme TL IM and lipase QLG, respectively, the DHA and EPA contents were trebled (from 22 to 69% for DHA, and from 19 to 61% for EPA). The stability of both lipases was also determined. Although Lipozyme TL IM is much more stable in isobutanol than in ethanol, with the former the conversion attained after four reaction cycles was about 40% of the initial conversion. In similar conditions, the conversion obtained with lipase QLG was about 88% of the initial conversion. In addition, the separation of DHA enriched acylglycerols and isobutyl esters from an alcoholysis reaction was studied by liquid–liquid fractionation using the ethanol–water–hexane biphasic system. The DHA enriched acylglycerols obtained were 97.6% pure (64.4% DHA). 2024-01-10T08:46:39Z 2024-01-10T08:46:39Z 2014-08-14 info:eu-repo/semantics/article https://doi.org/10.1016/j.bej.2014.08.010 1369-703X http://hdl.handle.net/10835/15037 en info:eu-repo/semantics/openAccess |
| spellingShingle | Docosahexaenoic acid (DHA) Eicosapentaenoic acid (EPA) Lipase Biokinetic Enzyme deactivation Liquid-liquid extraction Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Castillo López, Beatriz Robles Medina, Alfonso Navarro López, Elvira Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors |
| title | Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors |
| title_full | Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors |
| title_fullStr | Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors |
| title_full_unstemmed | Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors |
| title_short | Concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors |
| title_sort | concentration of docosahexaenoic and eicosapentaenoic acids by enzymatic alcoholysis with different acyl-acceptors |
| topic | Docosahexaenoic acid (DHA) Eicosapentaenoic acid (EPA) Lipase Biokinetic Enzyme deactivation Liquid-liquid extraction |
| url | https://doi.org/10.1016/j.bej.2014.08.010 http://hdl.handle.net/10835/15037 |
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