Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification
The aim of this work was to obtain biodiesel (methyl esters) from the saponifiable lipids (SLs) fraction of the microalga Nannochloropsis gaditana, whose biomass dry weight contains 12.1 wt% of these lipids. SLs were extracted from the microalga as free fatty acids (FFAs) for subsequent transformati...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
2024
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/15043 |
| _version_ | 1789406536463810560 |
|---|---|
| author | Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio |
| author_facet | Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio |
| author_sort | Castillo López, Beatriz |
| collection | DSpace |
| description | The aim of this work was to obtain biodiesel (methyl esters) from the saponifiable lipids (SLs) fraction of the microalga Nannochloropsis gaditana, whose biomass dry weight contains 12.1 wt% of these lipids. SLs were extracted from the microalga as free fatty acids (FFAs) for subsequent transformation to methyl esters (biodiesel) by enzymatic esterification. Extraction as FFAs rather than as SLs allows them to be obtained with higher purity. Microalgal FFAs were obtained by direct saponification of lipids in the biomass and subsequent extraction-purification with hexane. Esterification of FFAs with methanol was catalysed by lipase Novozym 435 from Candida antarctica. Stability studies of this lipase in the operational conditions showed that the esterification degree (ED) attained with the same batch of lipase remained constant over six reaction cycles (36 h total reaction time). The optimal conditions attained for 4 g of FFAs were 25 ºC, 200 rpm, methanol/FFA molar ratio of 1.5:1, Novozym 435/FFA ratio of 0.025:1 w/w and 4 h reaction time. In these conditions the ED attained was 92.6%, producing a biodiesel with 83 wt% purity from microalgal FFAs. Several experimental scales were tested (from 4 to 40 g FFAs), and in all cases similar EDs were obtained. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15043 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-150432024-01-10T09:01:04Z Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio biodiesel microalga Nannochloropsis gaditana lipase Novozym 435 free fatty acid used vegetable oil The aim of this work was to obtain biodiesel (methyl esters) from the saponifiable lipids (SLs) fraction of the microalga Nannochloropsis gaditana, whose biomass dry weight contains 12.1 wt% of these lipids. SLs were extracted from the microalga as free fatty acids (FFAs) for subsequent transformation to methyl esters (biodiesel) by enzymatic esterification. Extraction as FFAs rather than as SLs allows them to be obtained with higher purity. Microalgal FFAs were obtained by direct saponification of lipids in the biomass and subsequent extraction-purification with hexane. Esterification of FFAs with methanol was catalysed by lipase Novozym 435 from Candida antarctica. Stability studies of this lipase in the operational conditions showed that the esterification degree (ED) attained with the same batch of lipase remained constant over six reaction cycles (36 h total reaction time). The optimal conditions attained for 4 g of FFAs were 25 ºC, 200 rpm, methanol/FFA molar ratio of 1.5:1, Novozym 435/FFA ratio of 0.025:1 w/w and 4 h reaction time. In these conditions the ED attained was 92.6%, producing a biodiesel with 83 wt% purity from microalgal FFAs. Several experimental scales were tested (from 4 to 40 g FFAs), and in all cases similar EDs were obtained. 2024-01-10T09:01:04Z 2024-01-10T09:01:04Z 2015-06-01 info:eu-repo/semantics/article 13891723 http://hdl.handle.net/10835/15043 en https://doi.org/10.1016/j.jbiosc.2014.11.002 info:eu-repo/semantics/openAccess |
| spellingShingle | biodiesel microalga Nannochloropsis gaditana lipase Novozym 435 free fatty acid used vegetable oil Castillo López, Beatriz Esteban Cerdán, Luis Robles Medina, Alfonso Navarro López, Elvira Martín Valverde, Lorena Hita Peña, Estrella González Moreno, Pedro Antonio Molina Grima, Emilio Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
| title | Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
| title_full | Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
| title_fullStr | Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
| title_full_unstemmed | Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
| title_short | Production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
| title_sort | production of biodiesel from vegetable oil and microalgae by fatty acid extraction and enzymatic esterification |
| topic | biodiesel microalga Nannochloropsis gaditana lipase Novozym 435 free fatty acid used vegetable oil |
| url | http://hdl.handle.net/10835/15043 |
| work_keys_str_mv | AT castillolopezbeatriz productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification AT estebancerdanluis productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification AT roblesmedinaalfonso productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification AT navarrolopezelvira productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification AT martinvalverdelorena productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification AT hitapenaestrella productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification AT gonzalezmorenopedroantonio productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification AT molinagrimaemilio productionofbiodieselfromvegetableoilandmicroalgaebyfattyacidextractionandenzymaticesterification |