Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium
The aim of this work was to produce docosahexaenoic acid (DHA)-enriched acylglycerols through the alcoholysis of tuna oil (24-26% DHA) using ethanol, dodecanol and isobutanol as the acyl-acceptors. The alcoholysis reaction was catalyzed using Thermomyces lanuginosus lipase (Lipozyme TL IM) and was c...
| Main Authors: | , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/15147 https://doi.org/10.1016/j.molcatb.2015.07.010 |
| _version_ | 1789406662120964096 |
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| author | Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Robles Medina, Alfonso |
| author_facet | Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Robles Medina, Alfonso |
| author_sort | Martín Valverde, Lorena |
| collection | DSpace |
| description | The aim of this work was to produce docosahexaenoic acid (DHA)-enriched acylglycerols through the alcoholysis of tuna oil (24-26% DHA) using ethanol, dodecanol and isobutanol as the acyl-acceptors. The alcoholysis reaction was catalyzed using Thermomyces lanuginosus lipase (Lipozyme TL IM) and was carried out both in solvent-free medium and using tert-butanol to increase lipase stability. Studies were carried out to determine the influence of the reaction time and the lipase/oil ratio. The tuna oil’s DHA content was trebled (from 26 to 78 wt%) using isobutanol as the acyl acceptor while carrying out the alcoholysis reactions in solvent-free medium. However, under these conditions, the lipase was partially deactivated given that the conversion rate decreased by around 80% after catalyzing six successive reactions using the same lipase batch. With tert-butanol as the reaction medium, the lipase was more stable but the resulting DHA concentrations were lower: 56% with isobutanol and dodecanol (59% with dodecanol in solvent-free medium) and 42.5% with ethanol (47.5 % without solvent). |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15147 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-151472024-01-16T08:48:21Z Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Robles Medina, Alfonso Docosahexaenoic acid (DHA) lipase enzyme deactivation acyl-acceptor tert-butanol The aim of this work was to produce docosahexaenoic acid (DHA)-enriched acylglycerols through the alcoholysis of tuna oil (24-26% DHA) using ethanol, dodecanol and isobutanol as the acyl-acceptors. The alcoholysis reaction was catalyzed using Thermomyces lanuginosus lipase (Lipozyme TL IM) and was carried out both in solvent-free medium and using tert-butanol to increase lipase stability. Studies were carried out to determine the influence of the reaction time and the lipase/oil ratio. The tuna oil’s DHA content was trebled (from 26 to 78 wt%) using isobutanol as the acyl acceptor while carrying out the alcoholysis reactions in solvent-free medium. However, under these conditions, the lipase was partially deactivated given that the conversion rate decreased by around 80% after catalyzing six successive reactions using the same lipase batch. With tert-butanol as the reaction medium, the lipase was more stable but the resulting DHA concentrations were lower: 56% with isobutanol and dodecanol (59% with dodecanol in solvent-free medium) and 42.5% with ethanol (47.5 % without solvent). 2024-01-16T08:48:21Z 2024-01-16T08:48:21Z 2015-07-16 info:eu-repo/semantics/article http://hdl.handle.net/10835/15147 https://doi.org/10.1016/j.molcatb.2015.07.010 en info:eu-repo/semantics/openAccess |
| spellingShingle | Docosahexaenoic acid (DHA) lipase enzyme deactivation acyl-acceptor tert-butanol Martín Valverde, Lorena González Moreno, Pedro Antonio Esteban Cerdán, Luis Navarro López, Elvira Robles Medina, Alfonso Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium |
| title | Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium |
| title_full | Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium |
| title_fullStr | Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium |
| title_full_unstemmed | Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium |
| title_short | Concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium |
| title_sort | concentration of docosahexaenoic acid by enzymatic alcoholysis with different acyl-acceptors, using tert-butanol as reaction medium |
| topic | Docosahexaenoic acid (DHA) lipase enzyme deactivation acyl-acceptor tert-butanol |
| url | http://hdl.handle.net/10835/15147 https://doi.org/10.1016/j.molcatb.2015.07.010 |
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