Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis
This paper demonstrates a sequential partitioning method for isolating bioactive compounds from Chrysochromulina rotalis using a polarity gradient, replacing classic and hazardous solvents with greener alternatives. Seventeen solvents were evaluated based on their Hansen solubility parameters and fo...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/15218 https://doi.org/10.1016/j.biortech.2023.129057 |
| _version_ | 1789406298692911104 |
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| author | González Cardoso, Miguel Ángel Cerón García, María Del Carmen Navarro López, Elvira Molina Miras, Alejandro Contreras Gómez, Antonio García Camacho, Francisco |
| author_facet | González Cardoso, Miguel Ángel Cerón García, María Del Carmen Navarro López, Elvira Molina Miras, Alejandro Contreras Gómez, Antonio García Camacho, Francisco |
| author_sort | González Cardoso, Miguel Ángel |
| collection | DSpace |
| description | This paper demonstrates a sequential partitioning method for isolating bioactive compounds from Chrysochromulina rotalis using a polarity gradient, replacing classic and hazardous solvents with greener alternatives. Seventeen solvents were evaluated based on their Hansen solubility parameters and for having a similar polarity to the solvents they would replace, four of which were selected as substitutes in the classic fractionation process. Considering the fatty acid and carotenoid recovery yields obtained for each of the solvents, it has been proposed to replace hexane (HEX), toluene (TOL), dichloromethane (DCM) and n-butanol (BUT) with cyclohexane, chlorobenzene, isobutyl acetate and isoamyl alcohol, respectively. In addition, cytotoxic activity was observed when the TOL and DCM solvent extracts were tested against tumour cell lines, demonstrating the antiproliferative potential of compounds containing, for example, fucoxanthin, fatty acids, peptides, isoflavonoids or terpenes, among others. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15218 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-152182024-01-17T11:04:11Z Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis González Cardoso, Miguel Ángel Cerón García, María Del Carmen Navarro López, Elvira Molina Miras, Alejandro Contreras Gómez, Antonio García Camacho, Francisco Chrysochromulina Fractionation Alternative solvents Antiproliferative This paper demonstrates a sequential partitioning method for isolating bioactive compounds from Chrysochromulina rotalis using a polarity gradient, replacing classic and hazardous solvents with greener alternatives. Seventeen solvents were evaluated based on their Hansen solubility parameters and for having a similar polarity to the solvents they would replace, four of which were selected as substitutes in the classic fractionation process. Considering the fatty acid and carotenoid recovery yields obtained for each of the solvents, it has been proposed to replace hexane (HEX), toluene (TOL), dichloromethane (DCM) and n-butanol (BUT) with cyclohexane, chlorobenzene, isobutyl acetate and isoamyl alcohol, respectively. In addition, cytotoxic activity was observed when the TOL and DCM solvent extracts were tested against tumour cell lines, demonstrating the antiproliferative potential of compounds containing, for example, fucoxanthin, fatty acids, peptides, isoflavonoids or terpenes, among others. 2024-01-17T11:04:11Z 2024-01-17T11:04:11Z 2023-04-17 info:eu-repo/semantics/article González-Cardoso, M. A., Cerón-García, M. C., Navarro-López, E., Molina-Miras, A., Sánchez-Mirón, A., Contreras-Gómez, A., & García-Camacho, F. (2023). Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis. Bioresource Technology, 379, 129057 0960-8524 http://hdl.handle.net/10835/15218 https://doi.org/10.1016/j.biortech.2023.129057 en https://www.sciencedirect.com/science/article/pii/S0960852423004832 info:eu-repo/semantics/openAccess Elsevier |
| spellingShingle | Chrysochromulina Fractionation Alternative solvents Antiproliferative González Cardoso, Miguel Ángel Cerón García, María Del Carmen Navarro López, Elvira Molina Miras, Alejandro Contreras Gómez, Antonio García Camacho, Francisco Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis |
| title | Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis |
| title_full | Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis |
| title_fullStr | Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis |
| title_full_unstemmed | Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis |
| title_short | Alternatives to classic solvents for the isolation of bioactive compounds from Chrysochromulina rotalis |
| title_sort | alternatives to classic solvents for the isolation of bioactive compounds from chrysochromulina rotalis |
| topic | Chrysochromulina Fractionation Alternative solvents Antiproliferative |
| url | http://hdl.handle.net/10835/15218 https://doi.org/10.1016/j.biortech.2023.129057 |
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