Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae
The extraction of three families of compounds (carotenoids, fatty acids and amphidinols) from the biomass of two strains of Amphidinium carterae (ACRN03 and Dn241EHU) was improved by tuning cell disruption and solvent extraction operations. The extraction of carotenoids was evaluated using alkaline...
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| 格式: | info:eu-repo/semantics/article |
| 语言: | English |
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Elsevier
2024
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| 主题: | |
| 在线阅读: | http://hdl.handle.net/10835/15230 https://doi.org/10.1016/j.biortech.2020.123518 |
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| author | López Rodríguez, Mercedes Cerón García, María Del Carmen López Rosales, Lorenzo Navarro López, Elvira Sánchez Mirón, Asterio mirón, asterio Molina Miras, Alejandro Ralha Abreu, Ana Cristina Fernández De Las Nieves, Ignacio García Camacho, Francisco |
| author_facet | López Rodríguez, Mercedes Cerón García, María Del Carmen López Rosales, Lorenzo Navarro López, Elvira Sánchez Mirón, Asterio mirón, asterio Molina Miras, Alejandro Ralha Abreu, Ana Cristina Fernández De Las Nieves, Ignacio García Camacho, Francisco |
| author_sort | López Rodríguez, Mercedes |
| collection | DSpace |
| description | The extraction of three families of compounds (carotenoids, fatty acids and amphidinols) from the biomass of two strains of Amphidinium carterae (ACRN03 and Dn241EHU) was improved by tuning cell disruption and solvent extraction operations. The extraction of carotenoids was evaluated using alkaline saponification (0%-60% KOH d.w.) at different temperatures (25–80 °C). High levels of carotenoids were obtained at 60 °C using freeze-dried biomass, not subjected to cell disruption methods. The ACRN03 strain required 20% KOH whereas the Dn241EHU strain did not require saponification since carotenoid degradation was observed. The extraction efficiencies were determined with a wide range of pure solvents and mixtures thereof. Two empirical non-linear equations were used to correlate extraction percentages for each family of compounds with the Hildebrand solubility parameter (δT) and the polarity index of the solvents (PI). Thresholds of δT and PI of around 20 MPa1/2 and 6, respectively, were determined for the extraction of amphidinols, consistent with antiproliferative activity measurements. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15230 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-152302024-01-17T11:53:23Z Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae López Rodríguez, Mercedes Cerón García, María Del Carmen López Rosales, Lorenzo Navarro López, Elvira Sánchez Mirón, Asterio mirón, asterio Molina Miras, Alejandro Ralha Abreu, Ana Cristina Fernández De Las Nieves, Ignacio García Camacho, Francisco Extraction process polyunsaturated fatty acids carotenoids polarity index solubility parameters peridinin amphidinols The extraction of three families of compounds (carotenoids, fatty acids and amphidinols) from the biomass of two strains of Amphidinium carterae (ACRN03 and Dn241EHU) was improved by tuning cell disruption and solvent extraction operations. The extraction of carotenoids was evaluated using alkaline saponification (0%-60% KOH d.w.) at different temperatures (25–80 °C). High levels of carotenoids were obtained at 60 °C using freeze-dried biomass, not subjected to cell disruption methods. The ACRN03 strain required 20% KOH whereas the Dn241EHU strain did not require saponification since carotenoid degradation was observed. The extraction efficiencies were determined with a wide range of pure solvents and mixtures thereof. Two empirical non-linear equations were used to correlate extraction percentages for each family of compounds with the Hildebrand solubility parameter (δT) and the polarity index of the solvents (PI). Thresholds of δT and PI of around 20 MPa1/2 and 6, respectively, were determined for the extraction of amphidinols, consistent with antiproliferative activity measurements. 2024-01-17T11:53:23Z 2024-01-17T11:53:23Z 2020-05-07 info:eu-repo/semantics/article López-Rodríguez, M., Cerón-García, M. C., López-Rosales, L., Navarro-López, E., Sánchez-Mirón, A., Molina-Miras, A., Abreu, A.C., Fernámdez, I. & García-Camacho, F. (2020). Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae. Bioresource technology, 313, 123518. 0960-8524 http://hdl.handle.net/10835/15230 https://doi.org/10.1016/j.biortech.2020.123518 en https://www.sciencedirect.com/science/article/pii/S0960852420307902 info:eu-repo/semantics/openAccess Elsevier |
| spellingShingle | Extraction process polyunsaturated fatty acids carotenoids polarity index solubility parameters peridinin amphidinols López Rodríguez, Mercedes Cerón García, María Del Carmen López Rosales, Lorenzo Navarro López, Elvira Sánchez Mirón, Asterio mirón, asterio Molina Miras, Alejandro Ralha Abreu, Ana Cristina Fernández De Las Nieves, Ignacio García Camacho, Francisco Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae |
| title | Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae |
| title_full | Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae |
| title_fullStr | Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae |
| title_full_unstemmed | Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae |
| title_short | Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae |
| title_sort | improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga amphidinium carterae |
| topic | Extraction process polyunsaturated fatty acids carotenoids polarity index solubility parameters peridinin amphidinols |
| url | http://hdl.handle.net/10835/15230 https://doi.org/10.1016/j.biortech.2020.123518 |
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