Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia
Rationale: Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global...
| Main Authors: | , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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Thomas Lutz
2024
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| Online Access: | http://hdl.handle.net/10835/15264 https://doi.org/10.1016/j.physbeh.2021.113356 |
| _version_ | 1789406513611145216 |
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| author | Merchán Carrillo, Ana María Pérez Fernández, Cristian Antonio López López, María Josefa Moreno Casco, José Joaquín Moreno Montoya, Margarita Begoña Sánchez Santed, Luis Fernando Flores Cubos, María Del Pilar |
| author_facet | Merchán Carrillo, Ana María Pérez Fernández, Cristian Antonio López López, María Josefa Moreno Casco, José Joaquín Moreno Montoya, Margarita Begoña Sánchez Santed, Luis Fernando Flores Cubos, María Del Pilar |
| author_sort | Merchán Carrillo, Ana María |
| collection | DSpace |
| description | Rationale: Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global 5-H synthesis in the enteric and central nervous systems, suggesting a possible involvement of gut microbiota in compulsive spectrum disorders.
Objectives: The present study investigated whether chronic TRP depletion by diet alters the faecal bacterial community profiles of compulsive versus non-compulsive rats in schedule-induced polydipsia (SIP). Peripheral plasma 5-HT and brain-derived neurotrophic factor (BDNF) levels were evaluated.
Methods: Wistar rats were selected as High Drinkers (HD) or Low Drinkers (LD) according to their SIP behaviour and were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+). The faecal bacterial community structure was investigated with 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis.
Results: Compulsive HD rats showed a lower bacterial diversity than LD rats, irrespectively of the diet. The TRP-depleted HD rats, the only group increasing compulsive licking in SIP, showed a reduction of bacterial evenness and a highly functionally organized community compared with the other groups, indicating that this bacterial community is more fragile to external changes due to the dominance of a low number of species. The chronic TRP depletion by diet effectively reduced peripheral plasma 5-HT levels in both HD and LD rats, while plasma BDNF levels were not altered.
Conclusions: These results highlight the possible implication of reduced microbial diversity in compulsive behaviour and the involvement of the serotonergic system in modulating the gut brain-axis in compulsive spectrum disorders. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15264 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| publisher | Thomas Lutz |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-152642024-01-19T13:21:20Z Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia Merchán Carrillo, Ana María Pérez Fernández, Cristian Antonio López López, María Josefa Moreno Casco, José Joaquín Moreno Montoya, Margarita Begoña Sánchez Santed, Luis Fernando Flores Cubos, María Del Pilar Compulsivity Schedule-induced polydipsia Gut microbiota Chronic tryptophan depletion PCR-DGGE Brain-derived neurotrophic factor Rationale: Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global 5-H synthesis in the enteric and central nervous systems, suggesting a possible involvement of gut microbiota in compulsive spectrum disorders. Objectives: The present study investigated whether chronic TRP depletion by diet alters the faecal bacterial community profiles of compulsive versus non-compulsive rats in schedule-induced polydipsia (SIP). Peripheral plasma 5-HT and brain-derived neurotrophic factor (BDNF) levels were evaluated. Methods: Wistar rats were selected as High Drinkers (HD) or Low Drinkers (LD) according to their SIP behaviour and were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+). The faecal bacterial community structure was investigated with 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis. Results: Compulsive HD rats showed a lower bacterial diversity than LD rats, irrespectively of the diet. The TRP-depleted HD rats, the only group increasing compulsive licking in SIP, showed a reduction of bacterial evenness and a highly functionally organized community compared with the other groups, indicating that this bacterial community is more fragile to external changes due to the dominance of a low number of species. The chronic TRP depletion by diet effectively reduced peripheral plasma 5-HT levels in both HD and LD rats, while plasma BDNF levels were not altered. Conclusions: These results highlight the possible implication of reduced microbial diversity in compulsive behaviour and the involvement of the serotonergic system in modulating the gut brain-axis in compulsive spectrum disorders. 2024-01-19T13:21:20Z 2024-01-19T13:21:20Z 2021-02-10 info:eu-repo/semantics/article Merchán, A., Pérez-Fernández, C., López, M., Moreno, J., Moreno, M., Sánchez-Santed, F., & Flores, P. (2021). Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia. Physiology & Behavior, 233, 113356. https://doi.org/10.1016/j.physbeh.2021.113356 00319384 http://hdl.handle.net/10835/15264 https://doi.org/10.1016/j.physbeh.2021.113356 en PGC2018–099,117-B-C21 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Thomas Lutz Volume 233, 1 May 2021, 113356 |
| spellingShingle | Compulsivity Schedule-induced polydipsia Gut microbiota Chronic tryptophan depletion PCR-DGGE Brain-derived neurotrophic factor Merchán Carrillo, Ana María Pérez Fernández, Cristian Antonio López López, María Josefa Moreno Casco, José Joaquín Moreno Montoya, Margarita Begoña Sánchez Santed, Luis Fernando Flores Cubos, María Del Pilar Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia |
| title | Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia |
| title_full | Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia |
| title_fullStr | Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia |
| title_full_unstemmed | Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia |
| title_short | Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia |
| title_sort | dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia |
| topic | Compulsivity Schedule-induced polydipsia Gut microbiota Chronic tryptophan depletion PCR-DGGE Brain-derived neurotrophic factor |
| url | http://hdl.handle.net/10835/15264 https://doi.org/10.1016/j.physbeh.2021.113356 |
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