Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor
The aim of this work was to produce structured triacylglycerols (STAGs), with caprylic acid located at positions 1 and 3 of the glycerol backbone and docosohexaenoic acid (DHA) at position 2, by acidolysis of tuna oil and caprylic acid (CA) catalyzed by lipases Rd, from Rhizopus delemar, and Palatas...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
Biochemical Engineering Journal
2011
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/64 |
| _version_ | 1789406751522553856 |
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| author | Hita Peña, Estrella Robles Medina, Alfonso Camacho Páez, Belén González Moreno, Pedro Antonio Esteban Cerdán, Luis Jiménez Callejón, María José Muñío Martínez, María del Mar Molina Grima, Emilio |
| author_facet | Hita Peña, Estrella Robles Medina, Alfonso Camacho Páez, Belén González Moreno, Pedro Antonio Esteban Cerdán, Luis Jiménez Callejón, María José Muñío Martínez, María del Mar Molina Grima, Emilio |
| author_sort | Hita Peña, Estrella |
| collection | DSpace |
| description | The aim of this work was to produce structured triacylglycerols (STAGs), with caprylic acid located at positions 1 and 3 of the glycerol backbone and docosohexaenoic acid (DHA) at position 2, by acidolysis of tuna oil and caprylic acid (CA) catalyzed by lipases Rd, from Rhizopus delemar, and Palatase 20000L from Mucor miehei immobilized on Accurel MP1000 in a packed bed reactor (PBR), working in continuous and recirculation modes. First, different lipase/support ratios were tested for the immobilization of lipases and the best results were obtained with ratios of 0.67 (w/w) for lipase Rd and 6.67 (w/w) for Palatase. Both lipases were stable for at least 4 days in the operational conditions. In the storage conditions (5 ºC) lipases Rd and Palatase maintained constant activity for 5 and 1 month, respectively. These catalysts have been used to obtain STAGs by acidolysis of tuna oil and CA in a PBR operating with recirculation of the reaction mixture through the lipase bed. Thus, STAGs with 52-53% CA and 14-15% DHA were obtained. These results were the basis for establishing the operational conditions to obtain STAGs operating in continuous mode. These new conditions were established maintaining constant intensity of treatment (IOT, lipase amount × reaction time/oil amount). In this way STAGs with 44-50% CA and 17-24% DHA were obtained operating in continuous mode. Although the compositions of STAGs obtained with both lipases were similar, Palatase required an IOT about 4 times higher than lipase Rd. To separate the acidolysis products (free fatty acids, FFAs, and STAGs) an extraction method of FFAs by water-ethanol solutions was tested. The following variables were optimized: water/ethanol ratio (the best results were attained with a water/ethanol ratio of 30:70 w/w), the solvent/FFA-STAG mixture ratio (3:1 w/w) and the number of extraction steps (3 to 5). In these conditions highly pure STAGs (93-96%) were obtained with a yield of 85%. The residual FFAs can be eliminated by neutralization with a hydroethanolic KOH solution to obtain pure STAGs. The positional analysis of these STAGs, carried out by alcoholysis catalyzed by lipase Novozym 435, has shown that CA represents 55% of fatty acids located at positions 1 and 3 and DHA represents 42% of fatty acids at position 2. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-64 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2011 |
| publisher | Biochemical Engineering Journal |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-642023-04-12T19:33:43Z Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor Hita Peña, Estrella Robles Medina, Alfonso Camacho Páez, Belén González Moreno, Pedro Antonio Esteban Cerdán, Luis Jiménez Callejón, María José Muñío Martínez, María del Mar Molina Grima, Emilio structured lipids structured triacylglycerols acidolysis docosohexaenoic acid (DHA) caprylic acid immobilized lipase packed bed reactor tuna oil lipase from Rhizopus delemar Palatase 20000L The aim of this work was to produce structured triacylglycerols (STAGs), with caprylic acid located at positions 1 and 3 of the glycerol backbone and docosohexaenoic acid (DHA) at position 2, by acidolysis of tuna oil and caprylic acid (CA) catalyzed by lipases Rd, from Rhizopus delemar, and Palatase 20000L from Mucor miehei immobilized on Accurel MP1000 in a packed bed reactor (PBR), working in continuous and recirculation modes. First, different lipase/support ratios were tested for the immobilization of lipases and the best results were obtained with ratios of 0.67 (w/w) for lipase Rd and 6.67 (w/w) for Palatase. Both lipases were stable for at least 4 days in the operational conditions. In the storage conditions (5 ºC) lipases Rd and Palatase maintained constant activity for 5 and 1 month, respectively. These catalysts have been used to obtain STAGs by acidolysis of tuna oil and CA in a PBR operating with recirculation of the reaction mixture through the lipase bed. Thus, STAGs with 52-53% CA and 14-15% DHA were obtained. These results were the basis for establishing the operational conditions to obtain STAGs operating in continuous mode. These new conditions were established maintaining constant intensity of treatment (IOT, lipase amount × reaction time/oil amount). In this way STAGs with 44-50% CA and 17-24% DHA were obtained operating in continuous mode. Although the compositions of STAGs obtained with both lipases were similar, Palatase required an IOT about 4 times higher than lipase Rd. To separate the acidolysis products (free fatty acids, FFAs, and STAGs) an extraction method of FFAs by water-ethanol solutions was tested. The following variables were optimized: water/ethanol ratio (the best results were attained with a water/ethanol ratio of 30:70 w/w), the solvent/FFA-STAG mixture ratio (3:1 w/w) and the number of extraction steps (3 to 5). In these conditions highly pure STAGs (93-96%) were obtained with a yield of 85%. The residual FFAs can be eliminated by neutralization with a hydroethanolic KOH solution to obtain pure STAGs. The positional analysis of these STAGs, carried out by alcoholysis catalyzed by lipase Novozym 435, has shown that CA represents 55% of fatty acids located at positions 1 and 3 and DHA represents 42% of fatty acids at position 2. Ministerio de Educación y Ciencia (Spain), FEDER, Projects AGL2003-03335 and CTQ2007-64079 2011-07-13T07:10:26Z 2011-07-13T07:10:26Z 2009 info:eu-repo/semantics/article Biochemical Engineering Journal, 46 (2009) 257-264 http://hdl.handle.net/10835/64 en info:eu-repo/semantics/openAccess Biochemical Engineering Journal |
| spellingShingle | structured lipids structured triacylglycerols acidolysis docosohexaenoic acid (DHA) caprylic acid immobilized lipase packed bed reactor tuna oil lipase from Rhizopus delemar Palatase 20000L Hita Peña, Estrella Robles Medina, Alfonso Camacho Páez, Belén González Moreno, Pedro Antonio Esteban Cerdán, Luis Jiménez Callejón, María José Muñío Martínez, María del Mar Molina Grima, Emilio Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor |
| title | Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor |
| title_full | Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor |
| title_fullStr | Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor |
| title_full_unstemmed | Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor |
| title_short | Production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor |
| title_sort | production of structured triacylglycerols by acidolysis catalyzed by lipases immobilized in a packed bed reactor |
| topic | structured lipids structured triacylglycerols acidolysis docosohexaenoic acid (DHA) caprylic acid immobilized lipase packed bed reactor tuna oil lipase from Rhizopus delemar Palatase 20000L |
| url | http://hdl.handle.net/10835/64 |
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