Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae
Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte Lepidium subulatum to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci i...
| Main Authors: | , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | English |
| Published: |
MDPI
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/7375 |
| _version_ | 1789406518586638336 |
|---|---|
| author | Martínez Nieto, María Isabel Merlo Calvente, María Encarnación Mota Poveda, Juan Francisco Salmerón Sánchez, Esteban Segarra Moragues, José Gabriel |
| author_facet | Martínez Nieto, María Isabel Merlo Calvente, María Encarnación Mota Poveda, Juan Francisco Salmerón Sánchez, Esteban Segarra Moragues, José Gabriel |
| author_sort | Martínez Nieto, María Isabel |
| collection | DSpace |
| description | Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte Lepidium subulatum to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci including di- and tri-nucleotide repeats were characterized in L. subulatum. They amplified a total of 80 alleles (2–12 alleles per locus) in a sample of 35 individuals of L. subulatum, showing relatively high levels of genetic diversity, HO = 0.645, HE = 0.627. Cross-species transferability of all 12 loci was successful for the Iberian endemics Lepidium cardamines, Lepidium stylatum, and the widespread, Lepidium graminifolium and one species each of two related genera, Cardaria draba and Coronopus didymus. These microsatellite primers will be useful to investigate genetic diversity, population structure and to address conservation genetics in species of Lepidium. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-7375 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-73752023-04-12T18:58:49Z Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae Martínez Nieto, María Isabel Merlo Calvente, María Encarnación Mota Poveda, Juan Francisco Salmerón Sánchez, Esteban Segarra Moragues, José Gabriel Brassicaceae conservation genetics genetic diversity gypsophile next-generation sequencing SSR Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte Lepidium subulatum to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci including di- and tri-nucleotide repeats were characterized in L. subulatum. They amplified a total of 80 alleles (2–12 alleles per locus) in a sample of 35 individuals of L. subulatum, showing relatively high levels of genetic diversity, HO = 0.645, HE = 0.627. Cross-species transferability of all 12 loci was successful for the Iberian endemics Lepidium cardamines, Lepidium stylatum, and the widespread, Lepidium graminifolium and one species each of two related genera, Cardaria draba and Coronopus didymus. These microsatellite primers will be useful to investigate genetic diversity, population structure and to address conservation genetics in species of Lepidium. 2020-01-16T11:13:57Z 2020-01-16T11:13:57Z 2012-09-19 info:eu-repo/semantics/article 1422-0067 http://hdl.handle.net/10835/7375 en https://www.mdpi.com/1422-0067/13/9/11861 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | Brassicaceae conservation genetics genetic diversity gypsophile next-generation sequencing SSR Martínez Nieto, María Isabel Merlo Calvente, María Encarnación Mota Poveda, Juan Francisco Salmerón Sánchez, Esteban Segarra Moragues, José Gabriel Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae |
| title | Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae |
| title_full | Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae |
| title_fullStr | Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae |
| title_full_unstemmed | Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae |
| title_short | Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae |
| title_sort | microsatellite loci in the gypsophyte lepidium subulatum (brassicaceae), and transferability to other lepidieae |
| topic | Brassicaceae conservation genetics genetic diversity gypsophile next-generation sequencing SSR |
| url | http://hdl.handle.net/10835/7375 |
| work_keys_str_mv | AT martineznietomariaisabel microsatellitelociinthegypsophytelepidiumsubulatumbrassicaceaeandtransferabilitytootherlepidieae AT merlocalventemariaencarnacion microsatellitelociinthegypsophytelepidiumsubulatumbrassicaceaeandtransferabilitytootherlepidieae AT motapovedajuanfrancisco microsatellitelociinthegypsophytelepidiumsubulatumbrassicaceaeandtransferabilitytootherlepidieae AT salmeronsanchezesteban microsatellitelociinthegypsophytelepidiumsubulatumbrassicaceaeandtransferabilitytootherlepidieae AT segarramoraguesjosegabriel microsatellitelociinthegypsophytelepidiumsubulatumbrassicaceaeandtransferabilitytootherlepidieae |