SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells
Colorectal cancer (CRC) is one of the most common and mortal types of cancer. There is increasing evidence that some polyunsaturated fatty acids (PUFAs) exercise specific inhibitory actions on cancer cells through different mechanisms, as a previous study on CRC cells demonstrated for two very long-...
| Main Authors: | , , , , |
|---|---|
| פורמט: | info:eu-repo/semantics/article |
| שפה: | English |
| יצא לאור: |
MDPI
2020
|
| נושאים: | |
| גישה מקוונת: | http://hdl.handle.net/10835/7382 |
| _version_ | 1789406499504652288 |
|---|---|
| author | González Fernández, María José Fabrikov, Dmitri Ramos Bueno, Rebeca Pilar Guil Guerrero, José Luis Ortea, Ignacio |
| author_facet | González Fernández, María José Fabrikov, Dmitri Ramos Bueno, Rebeca Pilar Guil Guerrero, José Luis Ortea, Ignacio |
| author_sort | González Fernández, María José |
| collection | DSpace |
| description | Colorectal cancer (CRC) is one of the most common and mortal types of cancer. There is increasing evidence that some polyunsaturated fatty acids (PUFAs) exercise specific inhibitory actions on cancer cells through different mechanisms, as a previous study on CRC cells demonstrated for two very long-chain PUFA. These were docosahexaenoic acid (DHA, 22:6n3) and arachidonic acid (ARA, 20:4n6) in the free fatty acid (FFA) form. In this work, similar design and technology have been used to investigate the actions of both DHA and ARA as monoacylglycerol (MAG) molecules, and results have been compared with those obtained using the corresponding FFA. Cell assays revealed that ARA- and DHA-MAG exercised dose- and time-dependent antiproliferative actions, with DHA-MAG acting on cancer cells more efficiently than ARA-MAG. Sequential window acquisition of all theoretical mass spectra (SWATH)—mass spectrometry massive quantitative proteomics, validated by parallel reaction monitoring and followed by pathway analysis, revealed that DHA-MAG had a massive effect in the proteasome complex, while the ARA-MAG main effect was related to DNA replication. Prostaglandin synthesis also resulted as inhibited by DHA-MAG. Results clearly demonstrated the ability of both ARA- and DHA-MAG to induce cell death in colon cancer cells, which suggests a direct relationship between chemical structure and antitumoral actions. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-7382 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-73822023-04-12T18:54:26Z SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells González Fernández, María José Fabrikov, Dmitri Ramos Bueno, Rebeca Pilar Guil Guerrero, José Luis Ortea, Ignacio colorectal cancer proteomics SWATH docosahexaenoic acid arachidonic acid HT-29 cells monoacylglycerols Colorectal cancer (CRC) is one of the most common and mortal types of cancer. There is increasing evidence that some polyunsaturated fatty acids (PUFAs) exercise specific inhibitory actions on cancer cells through different mechanisms, as a previous study on CRC cells demonstrated for two very long-chain PUFA. These were docosahexaenoic acid (DHA, 22:6n3) and arachidonic acid (ARA, 20:4n6) in the free fatty acid (FFA) form. In this work, similar design and technology have been used to investigate the actions of both DHA and ARA as monoacylglycerol (MAG) molecules, and results have been compared with those obtained using the corresponding FFA. Cell assays revealed that ARA- and DHA-MAG exercised dose- and time-dependent antiproliferative actions, with DHA-MAG acting on cancer cells more efficiently than ARA-MAG. Sequential window acquisition of all theoretical mass spectra (SWATH)—mass spectrometry massive quantitative proteomics, validated by parallel reaction monitoring and followed by pathway analysis, revealed that DHA-MAG had a massive effect in the proteasome complex, while the ARA-MAG main effect was related to DNA replication. Prostaglandin synthesis also resulted as inhibited by DHA-MAG. Results clearly demonstrated the ability of both ARA- and DHA-MAG to induce cell death in colon cancer cells, which suggests a direct relationship between chemical structure and antitumoral actions. 2020-01-16T11:29:49Z 2020-01-16T11:29:49Z 2019-12-06 info:eu-repo/semantics/article 2072-6643 http://hdl.handle.net/10835/7382 en https://www.mdpi.com/2072-6643/11/12/2984 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | colorectal cancer proteomics SWATH docosahexaenoic acid arachidonic acid HT-29 cells monoacylglycerols González Fernández, María José Fabrikov, Dmitri Ramos Bueno, Rebeca Pilar Guil Guerrero, José Luis Ortea, Ignacio SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells |
| title | SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells |
| title_full | SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells |
| title_fullStr | SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells |
| title_full_unstemmed | SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells |
| title_short | SWATH Differential Abundance Proteomics and Cellular Assays Show In Vitro Anticancer Activity of Arachidonic Acid- and Docosahexaenoic Acid-Based Monoacylglycerols in HT-29 Colorectal Cancer Cells |
| title_sort | swath differential abundance proteomics and cellular assays show in vitro anticancer activity of arachidonic acid- and docosahexaenoic acid-based monoacylglycerols in ht-29 colorectal cancer cells |
| topic | colorectal cancer proteomics SWATH docosahexaenoic acid arachidonic acid HT-29 cells monoacylglycerols |
| url | http://hdl.handle.net/10835/7382 |
| work_keys_str_mv | AT gonzalezfernandezmariajose swathdifferentialabundanceproteomicsandcellularassaysshowinvitroanticanceractivityofarachidonicacidanddocosahexaenoicacidbasedmonoacylglycerolsinht29colorectalcancercells AT fabrikovdmitri swathdifferentialabundanceproteomicsandcellularassaysshowinvitroanticanceractivityofarachidonicacidanddocosahexaenoicacidbasedmonoacylglycerolsinht29colorectalcancercells AT ramosbuenorebecapilar swathdifferentialabundanceproteomicsandcellularassaysshowinvitroanticanceractivityofarachidonicacidanddocosahexaenoicacidbasedmonoacylglycerolsinht29colorectalcancercells AT guilguerrerojoseluis swathdifferentialabundanceproteomicsandcellularassaysshowinvitroanticanceractivityofarachidonicacidanddocosahexaenoicacidbasedmonoacylglycerolsinht29colorectalcancercells AT orteaignacio swathdifferentialabundanceproteomicsandcellularassaysshowinvitroanticanceractivityofarachidonicacidanddocosahexaenoicacidbasedmonoacylglycerolsinht29colorectalcancercells |