Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.)
Pod maturation of common bean relies upon complex gene expression changes, which in turn are crucial for seed formation and dispersal. Hence, dissecting the transcriptional regulation of pod maturation would be of great significance for breeding programs. In this study, a comprehensive characterizat...
| Main Authors: | , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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MDPI
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/8120 |
| _version_ | 1789406755323641856 |
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| author | Gómez Martín, Cristina Capel Salinas, Carmen González Fernández, Ana María Lebrón, Ricardo Yuste Lisbona, Fernando Juan Hackenberg, Michael Oliver Jiménez, José Lutgardo Santalla, Marta Lozano Ruiz, Rafael |
| author_facet | Gómez Martín, Cristina Capel Salinas, Carmen González Fernández, Ana María Lebrón, Ricardo Yuste Lisbona, Fernando Juan Hackenberg, Michael Oliver Jiménez, José Lutgardo Santalla, Marta Lozano Ruiz, Rafael |
| author_sort | Gómez Martín, Cristina |
| collection | DSpace |
| description | Pod maturation of common bean relies upon complex gene expression changes, which in turn are crucial for seed formation and dispersal. Hence, dissecting the transcriptional regulation of pod maturation would be of great significance for breeding programs. In this study, a comprehensive characterization of expression changes has been performed in two common bean cultivars (ancient and modern) by analyzing the transcriptomes of five developmental pod stages, from fruit setting to maturation. RNA-seq analysis allowed for the identification of key genes shared by both accessions, which in turn were homologous to known Arabidopsis maturation genes and furthermore showed a similar expression pattern along the maturation process. Gene- expression changes suggested a role in promoting an accelerated breakdown of photosynthetic and ribosomal machinery associated with chlorophyll degradation and early activation of alpha-linolenic acid metabolism. A further study of transcription factors and their DNA binding sites revealed three candidate genes whose functions may play a dominant role in regulating pod maturation. Altogether, this research identifies the first maturation gene set reported in common bean so far and contributes to a better understanding of the dynamic mechanisms of pod maturation, providing potentially useful information for genomic-assisted breeding of common bean yield and pod quality attributes. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-8120 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-81202023-04-12T19:00:53Z Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.) Gómez Martín, Cristina Capel Salinas, Carmen González Fernández, Ana María Lebrón, Ricardo Yuste Lisbona, Fernando Juan Hackenberg, Michael Oliver Jiménez, José Lutgardo Santalla, Marta Lozano Ruiz, Rafael pod maturation transcription factors photosynthesis metabolism Phaseolus vulgaris Pod maturation of common bean relies upon complex gene expression changes, which in turn are crucial for seed formation and dispersal. Hence, dissecting the transcriptional regulation of pod maturation would be of great significance for breeding programs. In this study, a comprehensive characterization of expression changes has been performed in two common bean cultivars (ancient and modern) by analyzing the transcriptomes of five developmental pod stages, from fruit setting to maturation. RNA-seq analysis allowed for the identification of key genes shared by both accessions, which in turn were homologous to known Arabidopsis maturation genes and furthermore showed a similar expression pattern along the maturation process. Gene- expression changes suggested a role in promoting an accelerated breakdown of photosynthetic and ribosomal machinery associated with chlorophyll degradation and early activation of alpha-linolenic acid metabolism. A further study of transcription factors and their DNA binding sites revealed three candidate genes whose functions may play a dominant role in regulating pod maturation. Altogether, this research identifies the first maturation gene set reported in common bean so far and contributes to a better understanding of the dynamic mechanisms of pod maturation, providing potentially useful information for genomic-assisted breeding of common bean yield and pod quality attributes. 2020-04-28T09:29:29Z 2020-04-28T09:29:29Z 2020-04-22 info:eu-repo/semantics/article 2223-7747 http://hdl.handle.net/10835/8120 en https://www.mdpi.com/2223-7747/9/4/545 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | pod maturation transcription factors photosynthesis metabolism Phaseolus vulgaris Gómez Martín, Cristina Capel Salinas, Carmen González Fernández, Ana María Lebrón, Ricardo Yuste Lisbona, Fernando Juan Hackenberg, Michael Oliver Jiménez, José Lutgardo Santalla, Marta Lozano Ruiz, Rafael Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.) |
| title | Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.) |
| title_full | Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.) |
| title_fullStr | Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.) |
| title_full_unstemmed | Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.) |
| title_short | Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.) |
| title_sort | transcriptional dynamics and candidate genes involved in pod maturation of common bean (phaseolus vulgaris l.) |
| topic | pod maturation transcription factors photosynthesis metabolism Phaseolus vulgaris |
| url | http://hdl.handle.net/10835/8120 |
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