LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then qu...

Full description

Bibliographic Details
Main Authors: Wellkamp, Marvin, García Camacho, Francisco, Durán Riveroll, Lorena M., Tebben, Jan, Tillmann, Urban, Krock, Bernd
Format: info:eu-repo/semantics/article
Language:English
Published: MDPI 2020
Subjects:
phycotoxin
ichthyotoxin
dinoflagellate
harmful algae
glycosylation
Online Access:http://hdl.handle.net/10835/8693
Description
Summary:Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.

Similar Items