LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then qu...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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MDPI
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10835/8693 |
| _version_ | 1789406481696686080 |
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| author | Wellkamp, Marvin García Camacho, Francisco Durán Riveroll, Lorena M. Tebben, Jan Tillmann, Urban Krock, Bernd |
| author_facet | Wellkamp, Marvin García Camacho, Francisco Durán Riveroll, Lorena M. Tebben, Jan Tillmann, Urban Krock, Bernd |
| author_sort | Wellkamp, Marvin |
| collection | DSpace |
| description | Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-8693 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-86932023-04-12T19:34:59Z LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium Wellkamp, Marvin García Camacho, Francisco Durán Riveroll, Lorena M. Tebben, Jan Tillmann, Urban Krock, Bernd phycotoxin ichthyotoxin dinoflagellate harmful algae glycosylation Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM. 2020-10-20T11:14:11Z 2020-10-20T11:14:11Z 2020-09-29 info:eu-repo/semantics/article 1660-3397 http://hdl.handle.net/10835/8693 en https://www.mdpi.com/1660-3397/18/10/497 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess MDPI |
| spellingShingle | phycotoxin ichthyotoxin dinoflagellate harmful algae glycosylation Wellkamp, Marvin García Camacho, Francisco Durán Riveroll, Lorena M. Tebben, Jan Tillmann, Urban Krock, Bernd LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium |
| title | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium |
| title_full | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium |
| title_fullStr | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium |
| title_full_unstemmed | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium |
| title_short | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium |
| title_sort | lc-ms/ms method development for the discovery and identification of amphidinols produced by amphidinium |
| topic | phycotoxin ichthyotoxin dinoflagellate harmful algae glycosylation |
| url | http://hdl.handle.net/10835/8693 |
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