Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water
This paper describes about an automated on-line enrichment method for the simultaneous determination of seven beta-blockers in river water using a short liquid chromatography column for preconcentration coupled with LC-DAD. The method performs the preconcentration of 30mL of river water samples (5%...
| Main Authors: | , , |
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| פורמט: | info:eu-repo/semantics/article |
| שפה: | English |
| יצא לאור: |
Elsevier
2011
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| נושאים: | |
| גישה מקוונת: | http://hdl.handle.net/10835/92 |
| _version_ | 1789406289482219520 |
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| author | Gil García, María Dolores Peñas Pedrosa, Beatriz Martínez Galera, María |
| author_facet | Gil García, María Dolores Peñas Pedrosa, Beatriz Martínez Galera, María |
| author_sort | Gil García, María Dolores |
| collection | DSpace |
| description | This paper describes about an automated on-line enrichment method for the simultaneous determination of seven beta-blockers in river water using a short liquid chromatography column for preconcentration coupled with LC-DAD. The method performs the preconcentration of 30mL of river water samples (5% organic modifier) using a 50mm×4.6mm C18 column for enrichment and a 150mm×4.6mm C18 column for separation, allowing the determination of beta-blockers at trace levels in river water. The analytical procedure was developed by optimizing the breakthrough parameters (flow rate, time of preconcentration and percentage of organic modifier added to the sample) in order to achieve the maximum sensitivity, and by optimizing the mobile phase (composition and flow rate) to get adequate separation of the components in a reasonable analysis time. Under the optimized conditions, the method was validated with respect to linearity, precision, limits of detection, limits of quantification and accuracy. Detection and quantitation limits ranged between 0.1 and 3.1 and between 1.0 and 5.0 ng mL−1, respectively, whereas the RSD on inter-day precision was below 8%. To cope with the matrix effect in the determination of these drugs in river water samples, the standard addition methodology was successfully applied. Recoveries ranging from 81 to 115% proved the accuracy of the methodology proposed in this work. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-92 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2011 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-922023-04-12T19:46:46Z Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water Gil García, María Dolores Peñas Pedrosa, Beatriz Martínez Galera, María Beta-blockers Environmental water On-line SPE-LC-LC-DAD Standard addition calibration This paper describes about an automated on-line enrichment method for the simultaneous determination of seven beta-blockers in river water using a short liquid chromatography column for preconcentration coupled with LC-DAD. The method performs the preconcentration of 30mL of river water samples (5% organic modifier) using a 50mm×4.6mm C18 column for enrichment and a 150mm×4.6mm C18 column for separation, allowing the determination of beta-blockers at trace levels in river water. The analytical procedure was developed by optimizing the breakthrough parameters (flow rate, time of preconcentration and percentage of organic modifier added to the sample) in order to achieve the maximum sensitivity, and by optimizing the mobile phase (composition and flow rate) to get adequate separation of the components in a reasonable analysis time. Under the optimized conditions, the method was validated with respect to linearity, precision, limits of detection, limits of quantification and accuracy. Detection and quantitation limits ranged between 0.1 and 3.1 and between 1.0 and 5.0 ng mL−1, respectively, whereas the RSD on inter-day precision was below 8%. To cope with the matrix effect in the determination of these drugs in river water samples, the standard addition methodology was successfully applied. Recoveries ranging from 81 to 115% proved the accuracy of the methodology proposed in this work. 2011-07-14T08:48:27Z 2011-07-14T08:48:27Z 2011 info:eu-repo/semantics/article Talanta 83 (2011) 1665-1672 0039-9140 http://hdl.handle.net/10835/92 en info:eu-repo/semantics/openAccess info:eu-repo/semantics/openAccess Elsevier |
| spellingShingle | Beta-blockers Environmental water On-line SPE-LC-LC-DAD Standard addition calibration Gil García, María Dolores Peñas Pedrosa, Beatriz Martínez Galera, María Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water |
| title | Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water |
| title_full | Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water |
| title_fullStr | Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water |
| title_full_unstemmed | Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water |
| title_short | Column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water |
| title_sort | column-switching linked to large sample volumes to preconcentrate beta-blockers at trace levels in environmental water |
| topic | Beta-blockers Environmental water On-line SPE-LC-LC-DAD Standard addition calibration |
| url | http://hdl.handle.net/10835/92 |
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