Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables
The coupled-column (LC-LC) system, consisting of a first column packed with internal surface reversed phase (ISRP) (50x4.6 mm ID) and a Chrompack C18 (100x4.6 mm ID) as second column, allowed the simultaneous determination of five benzoylurea insecticides in dichloromethane (CH2Cl2) extracts of vege...
| Main Authors: | , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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Wiley-Blackwell
2011
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| Online Access: | http://hdl.handle.net/10835/93 |
| _version_ | 1789406693561466880 |
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| author | Gil García, María Dolores Barranco Martínez, Dolores Martínez Galera, María Parrilla Vázquez, Piedad |
| author_facet | Gil García, María Dolores Barranco Martínez, Dolores Martínez Galera, María Parrilla Vázquez, Piedad |
| author_sort | Gil García, María Dolores |
| collection | DSpace |
| description | The coupled-column (LC-LC) system, consisting of a first column packed with internal surface reversed phase (ISRP) (50x4.6 mm ID) and a Chrompack C18 (100x4.6 mm ID) as second column, allowed the simultaneous determination of five benzoylurea insecticides in dichloromethane (CH2Cl2) extracts of vegetable samples without any clean-up step. This system was combined with a photochemically induced fluorescence (PIF) post-column derivatization in order to provide strongly fluorescent photoproducts from the non-fluorescent benzoylureas. Limits of detection ranged from 0.21 to 0.98 ug L–1 of pesticide (equivalent to 0.14–0.65 ug kg–1 in vegetable samples) and limits of determination ranged from 4.0 to 10.0 ug L–1 (equivalent to 2.7–6.7 ug kg–1). Linearity of the method was established between 2 and 1800 ug L–1, depending upon the compound. Validation of the total method was performed by randomly analyzing recoveries of four vegetable samples (aubergine, cucumber, green bean, and tomato) spiked at two levels of concentration (10.0 and 33.3 ug kg–1). The combination of the LC-LC system with PIF detection provides a sensitive, selective, and rapid method for the determination of pesticides in vegetable samples at levels lower than the maximum residue levels (MRLs) established for these compounds by Spanish legislation. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-93 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2011 |
| publisher | Wiley-Blackwell |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-932023-04-12T19:48:10Z Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables Gil García, María Dolores Barranco Martínez, Dolores Martínez Galera, María Parrilla Vázquez, Piedad Benzoylurea insecticides Photochemically induced fluorescence LC-LC separation Vegetables The coupled-column (LC-LC) system, consisting of a first column packed with internal surface reversed phase (ISRP) (50x4.6 mm ID) and a Chrompack C18 (100x4.6 mm ID) as second column, allowed the simultaneous determination of five benzoylurea insecticides in dichloromethane (CH2Cl2) extracts of vegetable samples without any clean-up step. This system was combined with a photochemically induced fluorescence (PIF) post-column derivatization in order to provide strongly fluorescent photoproducts from the non-fluorescent benzoylureas. Limits of detection ranged from 0.21 to 0.98 ug L–1 of pesticide (equivalent to 0.14–0.65 ug kg–1 in vegetable samples) and limits of determination ranged from 4.0 to 10.0 ug L–1 (equivalent to 2.7–6.7 ug kg–1). Linearity of the method was established between 2 and 1800 ug L–1, depending upon the compound. Validation of the total method was performed by randomly analyzing recoveries of four vegetable samples (aubergine, cucumber, green bean, and tomato) spiked at two levels of concentration (10.0 and 33.3 ug kg–1). The combination of the LC-LC system with PIF detection provides a sensitive, selective, and rapid method for the determination of pesticides in vegetable samples at levels lower than the maximum residue levels (MRLs) established for these compounds by Spanish legislation. 2011-07-22T07:25:40Z 2011-07-22T07:25:40Z 2004 info:eu-repo/semantics/article Journal of Separation Science 27 (2004) 1173-1180 1615-9306 http://hdl.handle.net/10835/93 en info:eu-repo/semantics/openAccess Wiley-Blackwell |
| spellingShingle | Benzoylurea insecticides Photochemically induced fluorescence LC-LC separation Vegetables Gil García, María Dolores Barranco Martínez, Dolores Martínez Galera, María Parrilla Vázquez, Piedad Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables |
| title | Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables |
| title_full | Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables |
| title_fullStr | Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables |
| title_full_unstemmed | Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables |
| title_short | Coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables |
| title_sort | coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables |
| topic | Benzoylurea insecticides Photochemically induced fluorescence LC-LC separation Vegetables |
| url | http://hdl.handle.net/10835/93 |
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